Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1050 and Pf1N1B4_1051 are separated by 226 nucleotides Pf1N1B4_1051 and Pf1N1B4_1052 are separated by 179 nucleotides Pf1N1B4_1052 and Pf1N1B4_1053 are separated by 40 nucleotides Pf1N1B4_1053 and Pf1N1B4_1054 are separated by 15 nucleotides
Pf1N1B4_1050 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 1,186,216 to 1,187,319
_1050
Pf1N1B4_1051 - Glutathione peroxidase family protein, at 1,187,546 to 1,187,974
_1051
Pf1N1B4_1052 - FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8), at 1,188,154 to 1,188,639
_1052
Pf1N1B4_1053 - hypothetical protein, at 1,188,680 to 1,188,991
_1053
Pf1N1B4_1054 - Phosphate acetyltransferase (EC 2.3.1.8), at 1,189,007 to 1,189,840
_1054
Position (kb)
1188
1189 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1187.189 kb on + strand, within Pf1N1B4_1050 at 1187.192 kb on + strand, within Pf1N1B4_1050 at 1187.192 kb on + strand, within Pf1N1B4_1050 at 1187.338 kb on + strand at 1187.338 kb on + strand at 1187.338 kb on + strand at 1187.339 kb on - strand at 1187.345 kb on + strand at 1187.346 kb on - strand at 1187.359 kb on + strand at 1187.389 kb on - strand at 1187.389 kb on - strand at 1187.396 kb on + strand at 1187.396 kb on + strand at 1187.396 kb on + strand at 1187.396 kb on + strand at 1187.396 kb on + strand at 1187.396 kb on + strand at 1187.397 kb on - strand at 1187.397 kb on - strand at 1187.397 kb on - strand at 1187.431 kb on - strand at 1187.475 kb on + strand at 1187.475 kb on + strand at 1187.476 kb on - strand at 1187.501 kb on + strand at 1187.599 kb on + strand, within Pf1N1B4_1051 at 1187.709 kb on + strand, within Pf1N1B4_1051 at 1187.709 kb on + strand, within Pf1N1B4_1051 at 1187.709 kb on + strand, within Pf1N1B4_1051 at 1187.710 kb on - strand, within Pf1N1B4_1051 at 1187.772 kb on + strand, within Pf1N1B4_1051 at 1187.772 kb on + strand, within Pf1N1B4_1051 at 1187.773 kb on - strand, within Pf1N1B4_1051 at 1187.773 kb on - strand, within Pf1N1B4_1051 at 1187.773 kb on - strand, within Pf1N1B4_1051 at 1187.874 kb on + strand, within Pf1N1B4_1051 at 1187.874 kb on + strand, within Pf1N1B4_1051 at 1187.874 kb on + strand, within Pf1N1B4_1051 at 1187.874 kb on + strand, within Pf1N1B4_1051 at 1187.874 kb on + strand at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.904 kb on + strand, within Pf1N1B4_1051 at 1187.905 kb on - strand, within Pf1N1B4_1051 at 1187.978 kb on + strand at 1187.979 kb on - strand at 1187.979 kb on - strand at 1187.979 kb on - strand at 1187.979 kb on - strand at 1187.979 kb on - strand at 1188.100 kb on - strand at 1188.428 kb on + strand, within Pf1N1B4_1052 at 1188.429 kb on - strand, within Pf1N1B4_1052 at 1188.429 kb on - strand, within Pf1N1B4_1052 at 1188.454 kb on + strand, within Pf1N1B4_1052 at 1188.455 kb on - strand, within Pf1N1B4_1052 at 1188.455 kb on - strand, within Pf1N1B4_1052 at 1188.522 kb on + strand, within Pf1N1B4_1052 at 1188.539 kb on + strand, within Pf1N1B4_1052 at 1188.601 kb on - strand at 1188.601 kb on - strand at 1188.602 kb on + strand at 1188.603 kb on - strand at 1188.603 kb on - strand at 1188.621 kb on + strand at 1188.871 kb on + strand, within Pf1N1B4_1053 at 1188.871 kb on + strand, within Pf1N1B4_1053 at 1188.968 kb on + strand at 1189.283 kb on + strand, within Pf1N1B4_1054 at 1189.283 kb on + strand, within Pf1N1B4_1054 at 1189.352 kb on + strand, within Pf1N1B4_1054 at 1189.352 kb on + strand, within Pf1N1B4_1054
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 1,187,189 + Pf1N1B4_1050 0.88 +0.7 1,187,192 + Pf1N1B4_1050 0.88 +0.7 1,187,192 + Pf1N1B4_1050 0.88 -1.7 1,187,338 + -0.4 1,187,338 + +1.3 1,187,338 + +0.7 1,187,339 - -1.3 1,187,345 + -0.7 1,187,346 - +0.7 1,187,359 + +0.8 1,187,389 - +0.3 1,187,389 - -1.7 1,187,396 + +1.3 1,187,396 + -2.9 1,187,396 + +0.3 1,187,396 + +0.6 1,187,396 + -0.3 1,187,396 + -2.1 1,187,397 - -0.3 1,187,397 - +0.8 1,187,397 - -0.5 1,187,431 - -1.1 1,187,475 + -1.3 1,187,475 + -0.7 1,187,476 - -1.3 1,187,501 + -1.7 1,187,599 + Pf1N1B4_1051 0.12 -1.3 1,187,709 + Pf1N1B4_1051 0.38 -0.7 1,187,709 + Pf1N1B4_1051 0.38 +0.7 1,187,709 + Pf1N1B4_1051 0.38 -0.7 1,187,710 - Pf1N1B4_1051 0.38 -0.7 1,187,772 + Pf1N1B4_1051 0.53 +1.3 1,187,772 + Pf1N1B4_1051 0.53 +2.1 1,187,773 - Pf1N1B4_1051 0.53 -1.1 1,187,773 - Pf1N1B4_1051 0.53 +1.7 1,187,773 - Pf1N1B4_1051 0.53 +0.9 1,187,874 + Pf1N1B4_1051 0.76 -2.3 1,187,874 + Pf1N1B4_1051 0.76 +0.3 1,187,874 + Pf1N1B4_1051 0.76 +1.4 1,187,874 + Pf1N1B4_1051 0.76 -0.3 1,187,874 + +2.3 1,187,875 - Pf1N1B4_1051 0.77 +1.3 1,187,875 - Pf1N1B4_1051 0.77 -1.3 1,187,875 - Pf1N1B4_1051 0.77 +2.6 1,187,875 - Pf1N1B4_1051 0.77 -0.1 1,187,875 - Pf1N1B4_1051 0.77 +1.1 1,187,875 - Pf1N1B4_1051 0.77 +1.8 1,187,875 - Pf1N1B4_1051 0.77 +1.1 1,187,904 + Pf1N1B4_1051 0.83 -0.8 1,187,905 - Pf1N1B4_1051 0.84 -1.2 1,187,978 + +1.7 1,187,979 - -3.5 1,187,979 - -0.2 1,187,979 - +3.3 1,187,979 - -0.1 1,187,979 - +0.3 1,188,100 - -1.1 1,188,428 + Pf1N1B4_1052 0.56 +1.3 1,188,429 - Pf1N1B4_1052 0.57 -1.1 1,188,429 - Pf1N1B4_1052 0.57 +0.6 1,188,454 + Pf1N1B4_1052 0.62 -2.1 1,188,455 - Pf1N1B4_1052 0.62 +0.0 1,188,455 - Pf1N1B4_1052 0.62 -2.3 1,188,522 + Pf1N1B4_1052 0.76 -1.1 1,188,539 + Pf1N1B4_1052 0.79 -1.7 1,188,601 - -1.2 1,188,601 - +0.3 1,188,602 + +0.0 1,188,603 - +1.8 1,188,603 - -0.1 1,188,621 + +1.3 1,188,871 + Pf1N1B4_1053 0.61 +0.8 1,188,871 + Pf1N1B4_1053 0.61 +0.3 1,188,968 + +3.0 1,189,283 + Pf1N1B4_1054 0.33 +0.3 1,189,283 + Pf1N1B4_1054 0.33 +0.1 1,189,352 + Pf1N1B4_1054 0.41 +0.8 1,189,352 + Pf1N1B4_1054 0.41 +0.3
Or see this region's nucleotide sequence