Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_9 and Pf1N1B4_10 are separated by 208 nucleotides Pf1N1B4_10 and Pf1N1B4_11 are separated by 280 nucleotides
Pf1N1B4_9 - Probable type IV pilus assembly FimV-related transmembrane protein, at 7,960 to 10,593
_9
Pf1N1B4_10 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 10,802 to 11,812
_10
Pf1N1B4_11 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 12,093 to 13,205
_11
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 10.104 kb on - strand, within Pf1N1B4_9 at 10.738 kb on + strand at 10.796 kb on + strand at 10.804 kb on - strand at 10.804 kb on - strand at 10.804 kb on - strand at 10.818 kb on - strand at 10.865 kb on + strand at 10.891 kb on + strand at 10.891 kb on + strand at 10.891 kb on + strand at 10.892 kb on - strand at 10.892 kb on - strand at 10.892 kb on - strand at 10.892 kb on - strand at 10.892 kb on - strand at 10.936 kb on - strand, within Pf1N1B4_10 at 11.107 kb on + strand, within Pf1N1B4_10 at 11.108 kb on - strand, within Pf1N1B4_10 at 11.108 kb on - strand, within Pf1N1B4_10 at 11.185 kb on + strand, within Pf1N1B4_10 at 11.186 kb on - strand, within Pf1N1B4_10 at 11.794 kb on + strand at 11.818 kb on + strand at 11.818 kb on + strand at 11.819 kb on - strand at 11.819 kb on - strand at 11.901 kb on - strand at 11.952 kb on + strand at 11.953 kb on - strand at 12.006 kb on + strand at 12.081 kb on + strand at 12.081 kb on + strand at 12.081 kb on + strand at 12.082 kb on - strand at 12.082 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 10,104 - Pf1N1B4_9 0.81 -2.2 10,738 + +0.5 10,796 + +0.1 10,804 - +2.4 10,804 - -1.1 10,804 - +3.3 10,818 - +3.3 10,865 + +0.8 10,891 + -0.2 10,891 + -0.2 10,891 + +2.1 10,892 - +0.1 10,892 - -0.2 10,892 - +1.4 10,892 - -0.2 10,892 - -3.0 10,936 - Pf1N1B4_10 0.13 -0.0 11,107 + Pf1N1B4_10 0.30 -0.6 11,108 - Pf1N1B4_10 0.30 -1.8 11,108 - Pf1N1B4_10 0.30 -1.2 11,185 + Pf1N1B4_10 0.38 -0.2 11,186 - Pf1N1B4_10 0.38 -1.8 11,794 + +0.1 11,818 + -0.9 11,818 + -2.5 11,819 - +0.8 11,819 - -1.8 11,901 - -1.2 11,952 + +0.2 11,953 - +1.2 12,006 + +2.8 12,081 + +0.9 12,081 + -0.2 12,081 + +0.8 12,082 - -0.4 12,082 - -0.2
Or see this region's nucleotide sequence