Experiment: Cephalothin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1313 and Ac3H11_1314 are separated by 13 nucleotides Ac3H11_1314 and Ac3H11_1315 are separated by 35 nucleotides Ac3H11_1315 and Ac3H11_1316 overlap by 4 nucleotides Ac3H11_1316 and Ac3H11_1317 are separated by 125 nucleotides
Ac3H11_1313 - Poly(A) polymerase (EC 2.7.7.19), at 903 to 2,513
_1313
Ac3H11_1314 - Phosphoserine phosphatase (EC 3.1.3.3), at 2,527 to 3,237
_1314
Ac3H11_1315 - Chromosomal replication initiator protein DnaA, at 3,273 to 3,977
_1315
Ac3H11_1316 - hypothetical protein, at 3,974 to 4,117
_1316
Ac3H11_1317 - Permeases of the major facilitator superfamily, at 4,243 to 5,460
_1317
Position (kb) 3
4 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 2.518 kb on - strand at 2.525 kb on - strand at 2.570 kb on - strand at 4.185 kb on + strand at 4.185 kb on + strand at 4.186 kb on - strand at 4.186 kb on - strand at 4.502 kb on - strand, within Ac3H11_1317 at 4.502 kb on - strand, within Ac3H11_1317 at 4.897 kb on + strand, within Ac3H11_1317 at 4.897 kb on + strand, within Ac3H11_1317 at 4.897 kb on + strand, within Ac3H11_1317 at 4.897 kb on + strand, within Ac3H11_1317 at 4.898 kb on - strand, within Ac3H11_1317
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml remove 2,518 - -0.5 2,525 - +0.4 2,570 - -0.4 4,185 + +0.5 4,185 + -1.1 4,186 - +0.2 4,186 - +0.9 4,502 - Ac3H11_1317 0.21 +0.3 4,502 - Ac3H11_1317 0.21 -0.7 4,897 + Ac3H11_1317 0.54 +4.4 4,897 + Ac3H11_1317 0.54 +1.1 4,897 + Ac3H11_1317 0.54 +0.1 4,897 + Ac3H11_1317 0.54 +0.5 4,898 - Ac3H11_1317 0.54 +0.2
Or see this region's nucleotide sequence