Experiment: MoYLS4 with Vanillin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0224 and DvMF_0225 are separated by 16 nucleotides DvMF_0225 and DvMF_0226 are separated by 315 nucleotides DvMF_0226 and DvMF_0227 are separated by 242 nucleotides
DvMF_0224: DvMF_0224 - glycine dehydrogenase subunit 1 (RefSeq), at 251,248 to 252,579
_0224
DvMF_0225: DvMF_0225 - glycine cleavage system protein H (RefSeq), at 252,596 to 252,979
_0225
DvMF_0226: DvMF_0226 - response regulator receiver protein (RefSeq), at 253,295 to 253,708
_0226
DvMF_0227: DvMF_0227 - phosphoglucomutase (RefSeq), at 253,951 to 255,600
_0227
Position (kb)
252
253 Strain fitness (log2 ratio)
-1
0
1 at 251.601 kb on + strand, within DvMF_0224 at 251.900 kb on + strand, within DvMF_0224 at 252.217 kb on - strand, within DvMF_0224 at 252.302 kb on + strand, within DvMF_0224 at 252.302 kb on + strand, within DvMF_0224 at 252.358 kb on + strand, within DvMF_0224 at 252.550 kb on - strand at 252.552 kb on + strand at 252.552 kb on + strand at 252.552 kb on + strand at 252.552 kb on + strand at 252.560 kb on - strand at 252.679 kb on + strand, within DvMF_0225 at 252.701 kb on - strand, within DvMF_0225 at 252.995 kb on + strand at 253.061 kb on + strand at 253.082 kb on - strand at 253.112 kb on - strand at 253.152 kb on - strand at 253.252 kb on + strand at 253.666 kb on + strand, within DvMF_0226 at 253.833 kb on + strand at 253.908 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vanillin 10 mM remove 251,601 + DvMF_0224 0.27 +0.3 251,900 + DvMF_0224 0.49 -0.6 252,217 - DvMF_0224 0.73 -0.8 252,302 + DvMF_0224 0.79 -0.7 252,302 + DvMF_0224 0.79 -0.9 252,358 + DvMF_0224 0.83 -0.7 252,550 - +0.1 252,552 + +0.4 252,552 + -0.4 252,552 + -0.7 252,552 + -0.2 252,560 - -0.1 252,679 + DvMF_0225 0.22 -0.8 252,701 - DvMF_0225 0.27 -0.8 252,995 + -0.6 253,061 + -0.3 253,082 - -0.8 253,112 - -1.0 253,152 - -0.6 253,252 + -0.2 253,666 + DvMF_0226 0.90 +0.6 253,833 + -0.5 253,908 + -1.4
Or see this region's nucleotide sequence