Experiment: MoYLS4 with Vanillin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0171 and DvMF_0172 are separated by 113 nucleotides DvMF_0172 and coaD overlap by 25 nucleotides coaD and DvMF_0174 are separated by 329 nucleotides
DvMF_0171: DvMF_0171 - beta-lactamase domain protein (RefSeq), at 187,304 to 188,914
_0171
DvMF_0172: DvMF_0172 - methyltransferase (RefSeq), at 189,028 to 189,600
_0172
DvMF_0173: coaD - phosphopantetheine adenylyltransferase (RefSeq), at 189,576 to 190,337
coaD
DvMF_0174: DvMF_0174 - tRNA delta(2)-isopentenylpyrophosphate transferase (RefSeq), at 190,667 to 191,680
_0174
Position (kb) 189
190
191 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 188.578 kb on + strand, within DvMF_0171 at 188.597 kb on - strand, within DvMF_0171 at 188.655 kb on + strand, within DvMF_0171 at 188.679 kb on + strand, within DvMF_0171 at 188.714 kb on + strand, within DvMF_0171 at 189.163 kb on - strand, within DvMF_0172 at 189.187 kb on - strand, within DvMF_0172 at 189.297 kb on + strand, within DvMF_0172 at 189.301 kb on - strand, within DvMF_0172 at 190.332 kb on + strand at 190.465 kb on - strand at 190.480 kb on - strand at 190.480 kb on - strand at 190.553 kb on - strand at 190.664 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vanillin 10 mM remove 188,578 + DvMF_0171 0.79 -0.7 188,597 - DvMF_0171 0.80 +0.0 188,655 + DvMF_0171 0.84 +0.1 188,679 + DvMF_0171 0.85 -0.2 188,714 + DvMF_0171 0.88 +0.5 189,163 - DvMF_0172 0.24 -1.7 189,187 - DvMF_0172 0.28 -2.9 189,297 + DvMF_0172 0.47 -1.8 189,301 - DvMF_0172 0.48 -1.7 190,332 + -0.6 190,465 - -1.0 190,480 - -0.8 190,480 - +2.6 190,553 - -0.2 190,664 + -0.3
Or see this region's nucleotide sequence