Experiment: Aluminum chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_520 and Pf1N1B4_521 are separated by 142 nucleotides Pf1N1B4_521 and Pf1N1B4_522 are separated by 189 nucleotides
Pf1N1B4_520 - Type I secretion system, outer membrane component LapE # AggA, at 617,438 to 618,796
_520
Pf1N1B4_521 - ABC transporter, transmembrane region:ABC transporter:Peptidase C39, bacteriocin processing, at 618,939 to 621,098
_521
Pf1N1B4_522 - HlyD family secretion protein, at 621,288 to 622,652
_522
Position (kb)
618
619
620
621
622 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 618.078 kb on - strand, within Pf1N1B4_520 at 618.078 kb on - strand, within Pf1N1B4_520 at 618.233 kb on + strand, within Pf1N1B4_520 at 618.258 kb on - strand at 618.295 kb on + strand, within Pf1N1B4_520 at 618.296 kb on - strand, within Pf1N1B4_520 at 618.302 kb on - strand, within Pf1N1B4_520 at 618.302 kb on - strand, within Pf1N1B4_520 at 618.356 kb on + strand, within Pf1N1B4_520 at 618.356 kb on + strand, within Pf1N1B4_520 at 618.357 kb on - strand, within Pf1N1B4_520 at 618.357 kb on - strand, within Pf1N1B4_520 at 618.357 kb on - strand, within Pf1N1B4_520 at 618.357 kb on - strand, within Pf1N1B4_520 at 618.357 kb on - strand, within Pf1N1B4_520 at 618.357 kb on - strand, within Pf1N1B4_520 at 618.626 kb on + strand, within Pf1N1B4_520 at 618.627 kb on - strand, within Pf1N1B4_520 at 618.627 kb on - strand, within Pf1N1B4_520 at 618.627 kb on - strand, within Pf1N1B4_520 at 618.627 kb on - strand, within Pf1N1B4_520 at 618.660 kb on - strand, within Pf1N1B4_520 at 618.680 kb on + strand at 618.762 kb on + strand at 618.762 kb on + strand at 618.799 kb on + strand at 618.799 kb on + strand at 618.799 kb on + strand at 618.799 kb on + strand at 618.799 kb on + strand at 618.800 kb on - strand at 618.939 kb on - strand at 618.939 kb on - strand at 619.324 kb on - strand, within Pf1N1B4_521 at 619.324 kb on - strand at 619.515 kb on + strand, within Pf1N1B4_521 at 620.169 kb on + strand, within Pf1N1B4_521 at 620.217 kb on + strand, within Pf1N1B4_521 at 620.217 kb on + strand, within Pf1N1B4_521 at 620.217 kb on + strand, within Pf1N1B4_521 at 620.217 kb on + strand, within Pf1N1B4_521 at 620.217 kb on + strand, within Pf1N1B4_521 at 620.217 kb on + strand at 620.217 kb on + strand, within Pf1N1B4_521 at 620.218 kb on - strand, within Pf1N1B4_521 at 620.218 kb on - strand, within Pf1N1B4_521 at 620.218 kb on - strand, within Pf1N1B4_521 at 620.413 kb on - strand, within Pf1N1B4_521 at 620.413 kb on - strand, within Pf1N1B4_521 at 620.413 kb on - strand, within Pf1N1B4_521 at 620.539 kb on - strand, within Pf1N1B4_521 at 620.539 kb on - strand, within Pf1N1B4_521 at 620.623 kb on - strand, within Pf1N1B4_521 at 620.689 kb on - strand, within Pf1N1B4_521 at 620.689 kb on - strand, within Pf1N1B4_521 at 620.843 kb on - strand, within Pf1N1B4_521 at 621.183 kb on + strand at 621.203 kb on + strand at 621.203 kb on + strand at 621.324 kb on + strand at 621.324 kb on + strand at 621.669 kb on + strand, within Pf1N1B4_522 at 621.669 kb on + strand, within Pf1N1B4_522 at 621.786 kb on + strand, within Pf1N1B4_522
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 2.5 mM remove 618,078 - Pf1N1B4_520 0.47 -0.8 618,078 - Pf1N1B4_520 0.47 -1.3 618,233 + Pf1N1B4_520 0.58 +0.4 618,258 - -0.1 618,295 + Pf1N1B4_520 0.63 -0.5 618,296 - Pf1N1B4_520 0.63 -0.9 618,302 - Pf1N1B4_520 0.64 -0.0 618,302 - Pf1N1B4_520 0.64 -1.7 618,356 + Pf1N1B4_520 0.68 -1.5 618,356 + Pf1N1B4_520 0.68 -1.0 618,357 - Pf1N1B4_520 0.68 +2.7 618,357 - Pf1N1B4_520 0.68 +0.9 618,357 - Pf1N1B4_520 0.68 +0.5 618,357 - Pf1N1B4_520 0.68 -0.5 618,357 - Pf1N1B4_520 0.68 +0.9 618,357 - Pf1N1B4_520 0.68 -2.1 618,626 + Pf1N1B4_520 0.87 +0.7 618,627 - Pf1N1B4_520 0.87 +0.9 618,627 - Pf1N1B4_520 0.87 +0.1 618,627 - Pf1N1B4_520 0.87 +0.9 618,627 - Pf1N1B4_520 0.87 +0.5 618,660 - Pf1N1B4_520 0.90 -1.0 618,680 + +0.5 618,762 + -0.1 618,762 + +0.6 618,799 + -0.7 618,799 + -0.8 618,799 + -2.1 618,799 + +0.2 618,799 + -0.7 618,800 - -1.7 618,939 - -0.3 618,939 - +0.6 619,324 - Pf1N1B4_521 0.18 -1.1 619,324 - +0.9 619,515 + Pf1N1B4_521 0.27 -1.5 620,169 + Pf1N1B4_521 0.57 -2.1 620,217 + Pf1N1B4_521 0.59 -0.1 620,217 + Pf1N1B4_521 0.59 -2.1 620,217 + Pf1N1B4_521 0.59 -1.3 620,217 + Pf1N1B4_521 0.59 -0.3 620,217 + Pf1N1B4_521 0.59 -0.5 620,217 + +1.7 620,217 + Pf1N1B4_521 0.59 +0.0 620,218 - Pf1N1B4_521 0.59 +0.3 620,218 - Pf1N1B4_521 0.59 -1.1 620,218 - Pf1N1B4_521 0.59 -1.2 620,413 - Pf1N1B4_521 0.68 -2.1 620,413 - Pf1N1B4_521 0.68 +0.3 620,413 - Pf1N1B4_521 0.68 -3.3 620,539 - Pf1N1B4_521 0.74 -0.7 620,539 - Pf1N1B4_521 0.74 +0.6 620,623 - Pf1N1B4_521 0.78 -0.1 620,689 - Pf1N1B4_521 0.81 -2.5 620,689 - Pf1N1B4_521 0.81 +0.6 620,843 - Pf1N1B4_521 0.88 -0.1 621,183 + -1.1 621,203 + +1.4 621,203 + +0.4 621,324 + +0.1 621,324 + -0.1 621,669 + Pf1N1B4_522 0.28 +1.9 621,669 + Pf1N1B4_522 0.28 -0.6 621,786 + Pf1N1B4_522 0.36 -0.1
Or see this region's nucleotide sequence