Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_521

Experiment: Aluminum chloride 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_520 and Pf1N1B4_521 are separated by 142 nucleotidesPf1N1B4_521 and Pf1N1B4_522 are separated by 189 nucleotides Pf1N1B4_520 - Type I secretion system, outer membrane component LapE # AggA, at 617,438 to 618,796 _520 Pf1N1B4_521 - ABC transporter, transmembrane region:ABC transporter:Peptidase C39, bacteriocin processing, at 618,939 to 621,098 _521 Pf1N1B4_522 - HlyD family secretion protein, at 621,288 to 622,652 _522 Position (kb) 618 619 620 621 622Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 618.078 kb on - strand, within Pf1N1B4_520at 618.078 kb on - strand, within Pf1N1B4_520at 618.233 kb on + strand, within Pf1N1B4_520at 618.258 kb on - strandat 618.295 kb on + strand, within Pf1N1B4_520at 618.296 kb on - strand, within Pf1N1B4_520at 618.302 kb on - strand, within Pf1N1B4_520at 618.302 kb on - strand, within Pf1N1B4_520at 618.356 kb on + strand, within Pf1N1B4_520at 618.356 kb on + strand, within Pf1N1B4_520at 618.357 kb on - strand, within Pf1N1B4_520at 618.357 kb on - strand, within Pf1N1B4_520at 618.357 kb on - strand, within Pf1N1B4_520at 618.357 kb on - strand, within Pf1N1B4_520at 618.357 kb on - strand, within Pf1N1B4_520at 618.357 kb on - strand, within Pf1N1B4_520at 618.626 kb on + strand, within Pf1N1B4_520at 618.627 kb on - strand, within Pf1N1B4_520at 618.627 kb on - strand, within Pf1N1B4_520at 618.627 kb on - strand, within Pf1N1B4_520at 618.627 kb on - strand, within Pf1N1B4_520at 618.660 kb on - strand, within Pf1N1B4_520at 618.680 kb on + strandat 618.762 kb on + strandat 618.762 kb on + strandat 618.799 kb on + strandat 618.799 kb on + strandat 618.799 kb on + strandat 618.799 kb on + strandat 618.799 kb on + strandat 618.800 kb on - strandat 618.939 kb on - strandat 618.939 kb on - strandat 619.324 kb on - strand, within Pf1N1B4_521at 619.324 kb on - strandat 619.515 kb on + strand, within Pf1N1B4_521at 620.169 kb on + strand, within Pf1N1B4_521at 620.217 kb on + strand, within Pf1N1B4_521at 620.217 kb on + strand, within Pf1N1B4_521at 620.217 kb on + strand, within Pf1N1B4_521at 620.217 kb on + strand, within Pf1N1B4_521at 620.217 kb on + strand, within Pf1N1B4_521at 620.217 kb on + strandat 620.217 kb on + strand, within Pf1N1B4_521at 620.218 kb on - strand, within Pf1N1B4_521at 620.218 kb on - strand, within Pf1N1B4_521at 620.218 kb on - strand, within Pf1N1B4_521at 620.413 kb on - strand, within Pf1N1B4_521at 620.413 kb on - strand, within Pf1N1B4_521at 620.413 kb on - strand, within Pf1N1B4_521at 620.539 kb on - strand, within Pf1N1B4_521at 620.539 kb on - strand, within Pf1N1B4_521at 620.623 kb on - strand, within Pf1N1B4_521at 620.689 kb on - strand, within Pf1N1B4_521at 620.689 kb on - strand, within Pf1N1B4_521at 620.843 kb on - strand, within Pf1N1B4_521at 621.183 kb on + strandat 621.203 kb on + strandat 621.203 kb on + strandat 621.324 kb on + strandat 621.324 kb on + strandat 621.669 kb on + strand, within Pf1N1B4_522at 621.669 kb on + strand, within Pf1N1B4_522at 621.786 kb on + strand, within Pf1N1B4_522

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Per-strain Table

Position Strand Gene LocusTag Fraction Aluminum chloride 2.5 mM
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618,078 - Pf1N1B4_520 0.47 -0.8
618,078 - Pf1N1B4_520 0.47 -1.3
618,233 + Pf1N1B4_520 0.58 +0.4
618,258 - -0.1
618,295 + Pf1N1B4_520 0.63 -0.5
618,296 - Pf1N1B4_520 0.63 -0.9
618,302 - Pf1N1B4_520 0.64 -0.0
618,302 - Pf1N1B4_520 0.64 -1.7
618,356 + Pf1N1B4_520 0.68 -1.5
618,356 + Pf1N1B4_520 0.68 -1.0
618,357 - Pf1N1B4_520 0.68 +2.7
618,357 - Pf1N1B4_520 0.68 +0.9
618,357 - Pf1N1B4_520 0.68 +0.5
618,357 - Pf1N1B4_520 0.68 -0.5
618,357 - Pf1N1B4_520 0.68 +0.9
618,357 - Pf1N1B4_520 0.68 -2.1
618,626 + Pf1N1B4_520 0.87 +0.7
618,627 - Pf1N1B4_520 0.87 +0.9
618,627 - Pf1N1B4_520 0.87 +0.1
618,627 - Pf1N1B4_520 0.87 +0.9
618,627 - Pf1N1B4_520 0.87 +0.5
618,660 - Pf1N1B4_520 0.90 -1.0
618,680 + +0.5
618,762 + -0.1
618,762 + +0.6
618,799 + -0.7
618,799 + -0.8
618,799 + -2.1
618,799 + +0.2
618,799 + -0.7
618,800 - -1.7
618,939 - -0.3
618,939 - +0.6
619,324 - Pf1N1B4_521 0.18 -1.1
619,324 - +0.9
619,515 + Pf1N1B4_521 0.27 -1.5
620,169 + Pf1N1B4_521 0.57 -2.1
620,217 + Pf1N1B4_521 0.59 -0.1
620,217 + Pf1N1B4_521 0.59 -2.1
620,217 + Pf1N1B4_521 0.59 -1.3
620,217 + Pf1N1B4_521 0.59 -0.3
620,217 + Pf1N1B4_521 0.59 -0.5
620,217 + +1.7
620,217 + Pf1N1B4_521 0.59 +0.0
620,218 - Pf1N1B4_521 0.59 +0.3
620,218 - Pf1N1B4_521 0.59 -1.1
620,218 - Pf1N1B4_521 0.59 -1.2
620,413 - Pf1N1B4_521 0.68 -2.1
620,413 - Pf1N1B4_521 0.68 +0.3
620,413 - Pf1N1B4_521 0.68 -3.3
620,539 - Pf1N1B4_521 0.74 -0.7
620,539 - Pf1N1B4_521 0.74 +0.6
620,623 - Pf1N1B4_521 0.78 -0.1
620,689 - Pf1N1B4_521 0.81 -2.5
620,689 - Pf1N1B4_521 0.81 +0.6
620,843 - Pf1N1B4_521 0.88 -0.1
621,183 + -1.1
621,203 + +1.4
621,203 + +0.4
621,324 + +0.1
621,324 + -0.1
621,669 + Pf1N1B4_522 0.28 +1.9
621,669 + Pf1N1B4_522 0.28 -0.6
621,786 + Pf1N1B4_522 0.36 -0.1

Or see this region's nucleotide sequence