Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4314

Experiment: Aluminum chloride 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4313 and Pf1N1B4_4314 are separated by 233 nucleotidesPf1N1B4_4314 and Pf1N1B4_4315 are separated by 43 nucleotidesPf1N1B4_4315 and Pf1N1B4_4316 are separated by 212 nucleotides Pf1N1B4_4313 - putative membrane protein, at 4,773,543 to 4,774,268 _4313 Pf1N1B4_4314 - ATP-dependent DNA ligase (EC 6.5.1.1) clustered with Ku protein, LigD, at 4,774,502 to 4,777,126 _4314 Pf1N1B4_4315 - hypothetical protein, at 4,777,170 to 4,777,451 _4315 Pf1N1B4_4316 - Ribosomal protein S3AE, at 4,777,664 to 4,777,999 _4316 Position (kb) 4774 4775 4776 4777 4778Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4773.571 kb on + strandat 4774.039 kb on + strand, within Pf1N1B4_4313at 4774.039 kb on + strandat 4774.039 kb on + strand, within Pf1N1B4_4313at 4774.040 kb on - strand, within Pf1N1B4_4313at 4774.040 kb on - strand, within Pf1N1B4_4313at 4774.040 kb on - strand, within Pf1N1B4_4313at 4774.040 kb on - strandat 4774.131 kb on + strand, within Pf1N1B4_4313at 4774.131 kb on + strand, within Pf1N1B4_4313at 4774.131 kb on + strand, within Pf1N1B4_4313at 4774.132 kb on - strandat 4774.132 kb on - strand, within Pf1N1B4_4313at 4774.182 kb on + strand, within Pf1N1B4_4313at 4774.182 kb on + strand, within Pf1N1B4_4313at 4774.183 kb on - strand, within Pf1N1B4_4313at 4774.183 kb on - strand, within Pf1N1B4_4313at 4774.183 kb on - strand, within Pf1N1B4_4313at 4774.231 kb on + strandat 4774.231 kb on + strandat 4774.231 kb on + strandat 4774.232 kb on - strandat 4774.232 kb on - strandat 4774.232 kb on - strandat 4774.232 kb on - strandat 4774.281 kb on + strandat 4774.293 kb on + strandat 4774.293 kb on + strandat 4774.360 kb on + strandat 4774.361 kb on - strandat 4774.362 kb on + strandat 4774.362 kb on + strandat 4774.363 kb on - strandat 4774.462 kb on + strandat 4774.462 kb on + strandat 4774.462 kb on + strandat 4774.462 kb on + strandat 4774.462 kb on + strandat 4774.463 kb on - strandat 4774.523 kb on + strandat 4774.649 kb on + strandat 4775.016 kb on - strand, within Pf1N1B4_4314at 4775.237 kb on + strand, within Pf1N1B4_4314at 4775.238 kb on - strand, within Pf1N1B4_4314at 4775.481 kb on - strand, within Pf1N1B4_4314at 4775.481 kb on - strand, within Pf1N1B4_4314at 4775.481 kb on - strand, within Pf1N1B4_4314at 4775.481 kb on - strand, within Pf1N1B4_4314at 4775.483 kb on + strand, within Pf1N1B4_4314at 4775.483 kb on + strand, within Pf1N1B4_4314at 4775.483 kb on + strand, within Pf1N1B4_4314at 4775.484 kb on - strand, within Pf1N1B4_4314at 4775.484 kb on - strandat 4775.484 kb on - strandat 4775.484 kb on - strand, within Pf1N1B4_4314at 4775.565 kb on + strand, within Pf1N1B4_4314at 4775.590 kb on - strand, within Pf1N1B4_4314at 4775.693 kb on + strand, within Pf1N1B4_4314at 4775.693 kb on + strand, within Pf1N1B4_4314at 4775.693 kb on + strand, within Pf1N1B4_4314at 4775.694 kb on - strand, within Pf1N1B4_4314at 4775.694 kb on - strand, within Pf1N1B4_4314at 4775.694 kb on - strand, within Pf1N1B4_4314at 4775.897 kb on + strand, within Pf1N1B4_4314at 4775.898 kb on - strand, within Pf1N1B4_4314at 4776.323 kb on + strand, within Pf1N1B4_4314at 4776.324 kb on - strand, within Pf1N1B4_4314at 4776.324 kb on - strand, within Pf1N1B4_4314at 4776.326 kb on + strand, within Pf1N1B4_4314at 4776.326 kb on + strand, within Pf1N1B4_4314at 4776.349 kb on - strand, within Pf1N1B4_4314at 4776.869 kb on - strandat 4776.869 kb on - strandat 4776.910 kb on + strandat 4776.911 kb on - strandat 4776.911 kb on - strandat 4777.124 kb on + strandat 4777.172 kb on - strandat 4777.447 kb on - strandat 4777.580 kb on - strandat 4777.641 kb on + strandat 4777.872 kb on + strand, within Pf1N1B4_4316at 4777.873 kb on - strand, within Pf1N1B4_4316at 4778.079 kb on - strandat 4778.087 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Aluminum chloride 2.5 mM
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4,773,571 + -0.5
4,774,039 + Pf1N1B4_4313 0.68 -2.1
4,774,039 + -1.5
4,774,039 + Pf1N1B4_4313 0.68 -1.1
4,774,040 - Pf1N1B4_4313 0.68 -2.1
4,774,040 - Pf1N1B4_4313 0.68 -0.5
4,774,040 - Pf1N1B4_4313 0.68 -0.5
4,774,040 - -1.5
4,774,131 + Pf1N1B4_4313 0.81 +1.5
4,774,131 + Pf1N1B4_4313 0.81 -1.6
4,774,131 + Pf1N1B4_4313 0.81 -0.8
4,774,132 - -0.5
4,774,132 - Pf1N1B4_4313 0.81 +0.7
4,774,182 + Pf1N1B4_4313 0.88 -0.5
4,774,182 + Pf1N1B4_4313 0.88 -0.3
4,774,183 - Pf1N1B4_4313 0.88 -2.5
4,774,183 - Pf1N1B4_4313 0.88 -1.5
4,774,183 - Pf1N1B4_4313 0.88 -0.9
4,774,231 + -1.2
4,774,231 + +1.1
4,774,231 + -1.5
4,774,232 - -0.9
4,774,232 - -0.9
4,774,232 - +0.8
4,774,232 - -0.5
4,774,281 + +0.8
4,774,293 + +2.2
4,774,293 + -1.5
4,774,360 + +0.1
4,774,361 - -3.3
4,774,362 + -0.5
4,774,362 + -0.1
4,774,363 - +0.5
4,774,462 + +1.1
4,774,462 + +0.5
4,774,462 + -1.1
4,774,462 + -0.5
4,774,462 + +0.1
4,774,463 - +1.1
4,774,523 + +0.9
4,774,649 + +1.5
4,775,016 - Pf1N1B4_4314 0.20 +0.1
4,775,237 + Pf1N1B4_4314 0.28 -2.1
4,775,238 - Pf1N1B4_4314 0.28 -0.5
4,775,481 - Pf1N1B4_4314 0.37 -3.5
4,775,481 - Pf1N1B4_4314 0.37 -1.2
4,775,481 - Pf1N1B4_4314 0.37 +0.5
4,775,481 - Pf1N1B4_4314 0.37 +0.1
4,775,483 + Pf1N1B4_4314 0.37 -2.1
4,775,483 + Pf1N1B4_4314 0.37 -1.5
4,775,483 + Pf1N1B4_4314 0.37 -1.5
4,775,484 - Pf1N1B4_4314 0.37 -3.1
4,775,484 - +0.5
4,775,484 - -0.5
4,775,484 - Pf1N1B4_4314 0.37 -0.5
4,775,565 + Pf1N1B4_4314 0.40 -1.5
4,775,590 - Pf1N1B4_4314 0.41 -0.7
4,775,693 + Pf1N1B4_4314 0.45 -1.5
4,775,693 + Pf1N1B4_4314 0.45 -1.5
4,775,693 + Pf1N1B4_4314 0.45 -1.1
4,775,694 - Pf1N1B4_4314 0.45 -0.1
4,775,694 - Pf1N1B4_4314 0.45 +0.2
4,775,694 - Pf1N1B4_4314 0.45 -1.2
4,775,897 + Pf1N1B4_4314 0.53 +2.1
4,775,898 - Pf1N1B4_4314 0.53 -1.1
4,776,323 + Pf1N1B4_4314 0.69 +0.3
4,776,324 - Pf1N1B4_4314 0.69 -1.7
4,776,324 - Pf1N1B4_4314 0.69 +1.5
4,776,326 + Pf1N1B4_4314 0.69 +0.1
4,776,326 + Pf1N1B4_4314 0.69 -3.1
4,776,349 - Pf1N1B4_4314 0.70 -1.5
4,776,869 - -0.5
4,776,869 - +1.1
4,776,910 + +2.3
4,776,911 - -0.1
4,776,911 - +0.5
4,777,124 + +1.1
4,777,172 - -3.3
4,777,447 - +0.5
4,777,580 - -0.1
4,777,641 + -0.2
4,777,872 + Pf1N1B4_4316 0.62 +0.3
4,777,873 - Pf1N1B4_4316 0.62 -0.9
4,778,079 - +0.5
4,778,087 - -0.3

Or see this region's nucleotide sequence