Experiment: Aluminum chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4313 and Pf1N1B4_4314 are separated by 233 nucleotides Pf1N1B4_4314 and Pf1N1B4_4315 are separated by 43 nucleotides Pf1N1B4_4315 and Pf1N1B4_4316 are separated by 212 nucleotides
Pf1N1B4_4313 - putative membrane protein, at 4,773,543 to 4,774,268
_4313
Pf1N1B4_4314 - ATP-dependent DNA ligase (EC 6.5.1.1) clustered with Ku protein, LigD, at 4,774,502 to 4,777,126
_4314
Pf1N1B4_4315 - hypothetical protein, at 4,777,170 to 4,777,451
_4315
Pf1N1B4_4316 - Ribosomal protein S3AE, at 4,777,664 to 4,777,999
_4316
Position (kb)
4774
4775
4776
4777
4778 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4773.571 kb on + strand at 4774.039 kb on + strand, within Pf1N1B4_4313 at 4774.039 kb on + strand at 4774.039 kb on + strand, within Pf1N1B4_4313 at 4774.040 kb on - strand, within Pf1N1B4_4313 at 4774.040 kb on - strand, within Pf1N1B4_4313 at 4774.040 kb on - strand, within Pf1N1B4_4313 at 4774.040 kb on - strand at 4774.131 kb on + strand, within Pf1N1B4_4313 at 4774.131 kb on + strand, within Pf1N1B4_4313 at 4774.131 kb on + strand, within Pf1N1B4_4313 at 4774.132 kb on - strand at 4774.132 kb on - strand, within Pf1N1B4_4313 at 4774.182 kb on + strand, within Pf1N1B4_4313 at 4774.182 kb on + strand, within Pf1N1B4_4313 at 4774.183 kb on - strand, within Pf1N1B4_4313 at 4774.183 kb on - strand, within Pf1N1B4_4313 at 4774.183 kb on - strand, within Pf1N1B4_4313 at 4774.231 kb on + strand at 4774.231 kb on + strand at 4774.231 kb on + strand at 4774.232 kb on - strand at 4774.232 kb on - strand at 4774.232 kb on - strand at 4774.232 kb on - strand at 4774.281 kb on + strand at 4774.293 kb on + strand at 4774.293 kb on + strand at 4774.360 kb on + strand at 4774.361 kb on - strand at 4774.362 kb on + strand at 4774.362 kb on + strand at 4774.363 kb on - strand at 4774.462 kb on + strand at 4774.462 kb on + strand at 4774.462 kb on + strand at 4774.462 kb on + strand at 4774.462 kb on + strand at 4774.463 kb on - strand at 4774.523 kb on + strand at 4774.649 kb on + strand at 4775.016 kb on - strand, within Pf1N1B4_4314 at 4775.237 kb on + strand, within Pf1N1B4_4314 at 4775.238 kb on - strand, within Pf1N1B4_4314 at 4775.481 kb on - strand, within Pf1N1B4_4314 at 4775.481 kb on - strand, within Pf1N1B4_4314 at 4775.481 kb on - strand, within Pf1N1B4_4314 at 4775.481 kb on - strand, within Pf1N1B4_4314 at 4775.483 kb on + strand, within Pf1N1B4_4314 at 4775.483 kb on + strand, within Pf1N1B4_4314 at 4775.483 kb on + strand, within Pf1N1B4_4314 at 4775.484 kb on - strand, within Pf1N1B4_4314 at 4775.484 kb on - strand at 4775.484 kb on - strand at 4775.484 kb on - strand, within Pf1N1B4_4314 at 4775.565 kb on + strand, within Pf1N1B4_4314 at 4775.590 kb on - strand, within Pf1N1B4_4314 at 4775.693 kb on + strand, within Pf1N1B4_4314 at 4775.693 kb on + strand, within Pf1N1B4_4314 at 4775.693 kb on + strand, within Pf1N1B4_4314 at 4775.694 kb on - strand, within Pf1N1B4_4314 at 4775.694 kb on - strand, within Pf1N1B4_4314 at 4775.694 kb on - strand, within Pf1N1B4_4314 at 4775.897 kb on + strand, within Pf1N1B4_4314 at 4775.898 kb on - strand, within Pf1N1B4_4314 at 4776.323 kb on + strand, within Pf1N1B4_4314 at 4776.324 kb on - strand, within Pf1N1B4_4314 at 4776.324 kb on - strand, within Pf1N1B4_4314 at 4776.326 kb on + strand, within Pf1N1B4_4314 at 4776.326 kb on + strand, within Pf1N1B4_4314 at 4776.349 kb on - strand, within Pf1N1B4_4314 at 4776.869 kb on - strand at 4776.869 kb on - strand at 4776.910 kb on + strand at 4776.911 kb on - strand at 4776.911 kb on - strand at 4777.124 kb on + strand at 4777.172 kb on - strand at 4777.447 kb on - strand at 4777.580 kb on - strand at 4777.641 kb on + strand at 4777.872 kb on + strand, within Pf1N1B4_4316 at 4777.873 kb on - strand, within Pf1N1B4_4316 at 4778.079 kb on - strand at 4778.087 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 2.5 mM remove 4,773,571 + -0.5 4,774,039 + Pf1N1B4_4313 0.68 -2.1 4,774,039 + -1.5 4,774,039 + Pf1N1B4_4313 0.68 -1.1 4,774,040 - Pf1N1B4_4313 0.68 -2.1 4,774,040 - Pf1N1B4_4313 0.68 -0.5 4,774,040 - Pf1N1B4_4313 0.68 -0.5 4,774,040 - -1.5 4,774,131 + Pf1N1B4_4313 0.81 +1.5 4,774,131 + Pf1N1B4_4313 0.81 -1.6 4,774,131 + Pf1N1B4_4313 0.81 -0.8 4,774,132 - -0.5 4,774,132 - Pf1N1B4_4313 0.81 +0.7 4,774,182 + Pf1N1B4_4313 0.88 -0.5 4,774,182 + Pf1N1B4_4313 0.88 -0.3 4,774,183 - Pf1N1B4_4313 0.88 -2.5 4,774,183 - Pf1N1B4_4313 0.88 -1.5 4,774,183 - Pf1N1B4_4313 0.88 -0.9 4,774,231 + -1.2 4,774,231 + +1.1 4,774,231 + -1.5 4,774,232 - -0.9 4,774,232 - -0.9 4,774,232 - +0.8 4,774,232 - -0.5 4,774,281 + +0.8 4,774,293 + +2.2 4,774,293 + -1.5 4,774,360 + +0.1 4,774,361 - -3.3 4,774,362 + -0.5 4,774,362 + -0.1 4,774,363 - +0.5 4,774,462 + +1.1 4,774,462 + +0.5 4,774,462 + -1.1 4,774,462 + -0.5 4,774,462 + +0.1 4,774,463 - +1.1 4,774,523 + +0.9 4,774,649 + +1.5 4,775,016 - Pf1N1B4_4314 0.20 +0.1 4,775,237 + Pf1N1B4_4314 0.28 -2.1 4,775,238 - Pf1N1B4_4314 0.28 -0.5 4,775,481 - Pf1N1B4_4314 0.37 -3.5 4,775,481 - Pf1N1B4_4314 0.37 -1.2 4,775,481 - Pf1N1B4_4314 0.37 +0.5 4,775,481 - Pf1N1B4_4314 0.37 +0.1 4,775,483 + Pf1N1B4_4314 0.37 -2.1 4,775,483 + Pf1N1B4_4314 0.37 -1.5 4,775,483 + Pf1N1B4_4314 0.37 -1.5 4,775,484 - Pf1N1B4_4314 0.37 -3.1 4,775,484 - +0.5 4,775,484 - -0.5 4,775,484 - Pf1N1B4_4314 0.37 -0.5 4,775,565 + Pf1N1B4_4314 0.40 -1.5 4,775,590 - Pf1N1B4_4314 0.41 -0.7 4,775,693 + Pf1N1B4_4314 0.45 -1.5 4,775,693 + Pf1N1B4_4314 0.45 -1.5 4,775,693 + Pf1N1B4_4314 0.45 -1.1 4,775,694 - Pf1N1B4_4314 0.45 -0.1 4,775,694 - Pf1N1B4_4314 0.45 +0.2 4,775,694 - Pf1N1B4_4314 0.45 -1.2 4,775,897 + Pf1N1B4_4314 0.53 +2.1 4,775,898 - Pf1N1B4_4314 0.53 -1.1 4,776,323 + Pf1N1B4_4314 0.69 +0.3 4,776,324 - Pf1N1B4_4314 0.69 -1.7 4,776,324 - Pf1N1B4_4314 0.69 +1.5 4,776,326 + Pf1N1B4_4314 0.69 +0.1 4,776,326 + Pf1N1B4_4314 0.69 -3.1 4,776,349 - Pf1N1B4_4314 0.70 -1.5 4,776,869 - -0.5 4,776,869 - +1.1 4,776,910 + +2.3 4,776,911 - -0.1 4,776,911 - +0.5 4,777,124 + +1.1 4,777,172 - -3.3 4,777,447 - +0.5 4,777,580 - -0.1 4,777,641 + -0.2 4,777,872 + Pf1N1B4_4316 0.62 +0.3 4,777,873 - Pf1N1B4_4316 0.62 -0.9 4,778,079 - +0.5 4,778,087 - -0.3
Or see this region's nucleotide sequence