Experiment: Aluminum chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_380 and Pf1N1B4_381 are separated by 293 nucleotides Pf1N1B4_381 and Pf1N1B4_382 are separated by 60 nucleotides Pf1N1B4_382 and Pf1N1B4_383 are separated by 364 nucleotides
Pf1N1B4_380 - Oxidoreductase, short chain dehydrogenase/reductase family, at 449,774 to 450,589
_380
Pf1N1B4_381 - hypothetical protein, at 450,883 to 451,035
_381
Pf1N1B4_382 - Phosphoglycerol transferase and related proteins, alkaline phosphatase superfamily, at 451,096 to 453,189
_382
Pf1N1B4_383 - Collagenase and related proteases, at 453,554 to 454,156
_383
Position (kb) 451
452
453
454 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 450.141 kb on + strand, within Pf1N1B4_380 at 450.141 kb on + strand, within Pf1N1B4_380 at 450.141 kb on + strand, within Pf1N1B4_380 at 450.141 kb on + strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.641 kb on - strand at 450.727 kb on - strand at 450.735 kb on + strand at 450.752 kb on + strand at 450.753 kb on - strand at 450.757 kb on + strand at 450.803 kb on - strand at 450.810 kb on + strand at 450.810 kb on + strand at 450.816 kb on + strand at 450.817 kb on - strand at 450.819 kb on + strand at 450.819 kb on + strand at 450.819 kb on + strand at 450.819 kb on + strand at 450.819 kb on + strand at 450.819 kb on + strand at 450.820 kb on - strand at 450.820 kb on - strand at 450.820 kb on - strand at 450.846 kb on + strand at 450.897 kb on + strand at 450.910 kb on - strand at 450.923 kb on + strand, within Pf1N1B4_381 at 450.923 kb on + strand, within Pf1N1B4_381 at 450.923 kb on + strand, within Pf1N1B4_381 at 450.923 kb on + strand, within Pf1N1B4_381 at 450.923 kb on + strand, within Pf1N1B4_381 at 450.924 kb on - strand, within Pf1N1B4_381 at 451.004 kb on + strand, within Pf1N1B4_381 at 451.020 kb on - strand at 451.031 kb on + strand at 451.032 kb on - strand at 451.032 kb on - strand at 451.033 kb on + strand at 451.033 kb on + strand at 451.033 kb on + strand at 451.083 kb on - strand at 451.250 kb on + strand at 451.250 kb on + strand at 451.251 kb on - strand at 451.251 kb on - strand at 451.251 kb on - strand at 451.461 kb on + strand, within Pf1N1B4_382 at 451.462 kb on - strand, within Pf1N1B4_382 at 451.608 kb on - strand, within Pf1N1B4_382 at 451.608 kb on - strand, within Pf1N1B4_382 at 451.608 kb on - strand, within Pf1N1B4_382 at 451.608 kb on - strand, within Pf1N1B4_382 at 451.772 kb on + strand, within Pf1N1B4_382 at 451.772 kb on + strand, within Pf1N1B4_382 at 451.772 kb on + strand, within Pf1N1B4_382 at 451.772 kb on + strand, within Pf1N1B4_382 at 451.773 kb on - strand, within Pf1N1B4_382 at 451.773 kb on - strand, within Pf1N1B4_382 at 451.890 kb on + strand, within Pf1N1B4_382 at 451.890 kb on + strand, within Pf1N1B4_382 at 451.891 kb on - strand, within Pf1N1B4_382 at 451.891 kb on - strand at 451.964 kb on + strand, within Pf1N1B4_382 at 451.964 kb on + strand, within Pf1N1B4_382 at 451.964 kb on + strand, within Pf1N1B4_382 at 451.965 kb on - strand, within Pf1N1B4_382 at 451.965 kb on - strand, within Pf1N1B4_382 at 451.965 kb on - strand, within Pf1N1B4_382 at 451.965 kb on - strand, within Pf1N1B4_382 at 451.965 kb on - strand, within Pf1N1B4_382 at 451.970 kb on + strand, within Pf1N1B4_382 at 451.970 kb on + strand at 451.970 kb on + strand, within Pf1N1B4_382 at 451.970 kb on + strand, within Pf1N1B4_382 at 451.970 kb on + strand, within Pf1N1B4_382 at 451.970 kb on + strand, within Pf1N1B4_382 at 451.970 kb on + strand, within Pf1N1B4_382 at 451.970 kb on - strand, within Pf1N1B4_382 at 451.971 kb on - strand, within Pf1N1B4_382 at 451.971 kb on - strand, within Pf1N1B4_382 at 451.971 kb on - strand, within Pf1N1B4_382 at 451.971 kb on - strand, within Pf1N1B4_382 at 452.171 kb on + strand at 452.172 kb on - strand, within Pf1N1B4_382 at 452.496 kb on - strand, within Pf1N1B4_382 at 452.954 kb on + strand at 452.954 kb on + strand, within Pf1N1B4_382 at 452.954 kb on + strand, within Pf1N1B4_382 at 452.955 kb on - strand, within Pf1N1B4_382 at 453.044 kb on + strand at 453.044 kb on + strand at 453.044 kb on + strand at 453.104 kb on - strand at 453.104 kb on - strand at 453.104 kb on - strand at 453.110 kb on + strand at 453.110 kb on + strand at 453.150 kb on + strand at 453.197 kb on + strand at 453.197 kb on + strand at 453.228 kb on + strand at 453.229 kb on - strand at 453.316 kb on + strand at 453.317 kb on - strand at 453.327 kb on - strand at 453.327 kb on - strand at 453.327 kb on - strand at 453.355 kb on + strand at 453.471 kb on - strand at 453.471 kb on - strand at 453.471 kb on - strand at 453.537 kb on - strand at 453.545 kb on + strand at 453.545 kb on + strand at 453.546 kb on - strand at 453.546 kb on - strand at 453.546 kb on - strand at 453.546 kb on - strand at 453.548 kb on - strand at 453.555 kb on + strand at 453.555 kb on + strand at 453.666 kb on + strand, within Pf1N1B4_383 at 453.666 kb on + strand at 453.666 kb on + strand, within Pf1N1B4_383 at 453.667 kb on - strand, within Pf1N1B4_383 at 453.667 kb on - strand, within Pf1N1B4_383 at 453.667 kb on - strand, within Pf1N1B4_383 at 453.667 kb on - strand, within Pf1N1B4_383 at 453.732 kb on - strand, within Pf1N1B4_383 at 453.735 kb on - strand, within Pf1N1B4_383 at 453.756 kb on + strand, within Pf1N1B4_383 at 453.756 kb on + strand at 453.756 kb on + strand, within Pf1N1B4_383 at 453.756 kb on + strand, within Pf1N1B4_383 at 453.757 kb on - strand, within Pf1N1B4_383 at 453.757 kb on - strand, within Pf1N1B4_383 at 453.757 kb on - strand, within Pf1N1B4_383 at 453.757 kb on - strand, within Pf1N1B4_383 at 453.870 kb on + strand, within Pf1N1B4_383 at 453.870 kb on + strand, within Pf1N1B4_383 at 453.870 kb on + strand, within Pf1N1B4_383 at 453.871 kb on - strand, within Pf1N1B4_383 at 453.871 kb on - strand, within Pf1N1B4_383 at 454.017 kb on + strand at 454.138 kb on + strand at 454.138 kb on + strand at 454.139 kb on - strand at 454.139 kb on - strand at 454.162 kb on + strand at 454.163 kb on - strand at 454.163 kb on - strand at 454.163 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 2.5 mM remove 450,141 + Pf1N1B4_380 0.45 +2.1 450,141 + Pf1N1B4_380 0.45 -0.2 450,141 + Pf1N1B4_380 0.45 +0.5 450,141 + Pf1N1B4_380 0.45 -0.8 450,142 - Pf1N1B4_380 0.45 +0.4 450,142 - Pf1N1B4_380 0.45 +1.0 450,142 - +0.1 450,142 - Pf1N1B4_380 0.45 -2.2 450,142 - Pf1N1B4_380 0.45 -1.2 450,142 - Pf1N1B4_380 0.45 -0.0 450,641 - +0.4 450,727 - -1.8 450,735 + -1.8 450,752 + -0.2 450,753 - -0.4 450,757 + +0.1 450,803 - +1.1 450,810 + -0.4 450,810 + -1.2 450,816 + -0.7 450,817 - -0.2 450,819 + -1.0 450,819 + -0.7 450,819 + -0.2 450,819 + +0.7 450,819 + -0.2 450,819 + -2.8 450,820 - +0.6 450,820 - +1.6 450,820 - +1.1 450,846 + +0.6 450,897 + +1.6 450,910 - -0.2 450,923 + Pf1N1B4_381 0.26 +0.6 450,923 + Pf1N1B4_381 0.26 +0.4 450,923 + Pf1N1B4_381 0.26 +1.4 450,923 + Pf1N1B4_381 0.26 -0.8 450,923 + Pf1N1B4_381 0.26 -0.2 450,924 - Pf1N1B4_381 0.27 -1.5 451,004 + Pf1N1B4_381 0.79 +1.8 451,020 - +1.8 451,031 + +1.2 451,032 - -0.2 451,032 - +2.0 451,033 + +0.8 451,033 + -0.2 451,033 + +1.6 451,083 - -0.2 451,250 + +1.0 451,250 + -1.5 451,251 - -0.2 451,251 - -0.8 451,251 - +0.1 451,461 + Pf1N1B4_382 0.17 -0.8 451,462 - Pf1N1B4_382 0.17 -0.2 451,608 - Pf1N1B4_382 0.24 -0.2 451,608 - Pf1N1B4_382 0.24 -0.8 451,608 - Pf1N1B4_382 0.24 -1.8 451,608 - Pf1N1B4_382 0.24 -0.8 451,772 + Pf1N1B4_382 0.32 -1.5 451,772 + Pf1N1B4_382 0.32 -3.2 451,772 + Pf1N1B4_382 0.32 +0.4 451,772 + Pf1N1B4_382 0.32 -2.0 451,773 - Pf1N1B4_382 0.32 +1.4 451,773 - Pf1N1B4_382 0.32 -1.8 451,890 + Pf1N1B4_382 0.38 -2.2 451,890 + Pf1N1B4_382 0.38 -2.2 451,891 - Pf1N1B4_382 0.38 -0.2 451,891 - +0.8 451,964 + Pf1N1B4_382 0.41 +1.1 451,964 + Pf1N1B4_382 0.41 -2.5 451,964 + Pf1N1B4_382 0.41 -1.8 451,965 - Pf1N1B4_382 0.41 -1.8 451,965 - Pf1N1B4_382 0.41 +0.6 451,965 - Pf1N1B4_382 0.41 -2.2 451,965 - Pf1N1B4_382 0.41 -2.2 451,965 - Pf1N1B4_382 0.41 -0.2 451,970 + Pf1N1B4_382 0.42 +0.1 451,970 + -1.2 451,970 + Pf1N1B4_382 0.42 -0.2 451,970 + Pf1N1B4_382 0.42 -2.8 451,970 + Pf1N1B4_382 0.42 -1.8 451,970 + Pf1N1B4_382 0.42 -0.2 451,970 + Pf1N1B4_382 0.42 -1.2 451,970 - Pf1N1B4_382 0.42 -2.2 451,971 - Pf1N1B4_382 0.42 -0.4 451,971 - Pf1N1B4_382 0.42 -0.8 451,971 - Pf1N1B4_382 0.42 -0.8 451,971 - Pf1N1B4_382 0.42 -0.2 452,171 + -0.2 452,172 - Pf1N1B4_382 0.51 +0.2 452,496 - Pf1N1B4_382 0.67 -0.6 452,954 + +2.1 452,954 + Pf1N1B4_382 0.89 -1.2 452,954 + Pf1N1B4_382 0.89 -0.2 452,955 - Pf1N1B4_382 0.89 -1.1 453,044 + +0.4 453,044 + -0.2 453,044 + -1.2 453,104 - -1.8 453,104 - -0.8 453,104 - -1.8 453,110 + +0.4 453,110 + +1.6 453,150 + +0.2 453,197 + -1.2 453,197 + -0.2 453,228 + +0.8 453,229 - -1.2 453,316 + -0.9 453,317 - -0.8 453,327 - +0.3 453,327 - +1.1 453,327 - +0.6 453,355 + -2.5 453,471 - +1.7 453,471 - +0.8 453,471 - -1.5 453,537 - -0.2 453,545 + -1.2 453,545 + -0.6 453,546 - -0.9 453,546 - -0.2 453,546 - -0.4 453,546 - +0.6 453,548 - -0.0 453,555 + -0.2 453,555 + +0.1 453,666 + Pf1N1B4_383 0.19 +1.1 453,666 + -0.2 453,666 + Pf1N1B4_383 0.19 -0.9 453,667 - Pf1N1B4_383 0.19 -1.8 453,667 - Pf1N1B4_383 0.19 -1.0 453,667 - Pf1N1B4_383 0.19 -2.5 453,667 - Pf1N1B4_383 0.19 -1.8 453,732 - Pf1N1B4_383 0.30 -0.0 453,735 - Pf1N1B4_383 0.30 +1.6 453,756 + Pf1N1B4_383 0.33 +0.2 453,756 + -0.2 453,756 + Pf1N1B4_383 0.33 -0.2 453,756 + Pf1N1B4_383 0.33 -0.2 453,757 - Pf1N1B4_383 0.34 +0.0 453,757 - Pf1N1B4_383 0.34 -0.2 453,757 - Pf1N1B4_383 0.34 -0.8 453,757 - Pf1N1B4_383 0.34 +1.8 453,870 + Pf1N1B4_383 0.52 +0.4 453,870 + Pf1N1B4_383 0.52 +3.1 453,870 + Pf1N1B4_383 0.52 +1.1 453,871 - Pf1N1B4_383 0.53 +0.2 453,871 - Pf1N1B4_383 0.53 -0.9 454,017 + -0.2 454,138 + -1.8 454,138 + -2.2 454,139 - +1.5 454,139 - +0.2 454,162 + +0.2 454,163 - +1.7 454,163 - -1.8 454,163 - -0.4
Or see this region's nucleotide sequence