Experiment: Aluminum chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3194 and Pf1N1B4_3195 are separated by 96 nucleotides Pf1N1B4_3195 and Pf1N1B4_3196 are separated by 227 nucleotides
Pf1N1B4_3194 - ATP-dependent helicase DinG/Rad3, at 3,574,459 to 3,576,603
_3194
Pf1N1B4_3195 - hydrolase, putative, at 3,576,700 to 3,579,222
_3195
Pf1N1B4_3196 - Esterase A, at 3,579,450 to 3,580,595
_3196
Position (kb)
3576
3577
3578
3579
3580 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3575.887 kb on + strand, within Pf1N1B4_3194 at 3576.620 kb on + strand at 3576.620 kb on + strand at 3576.620 kb on + strand at 3576.620 kb on + strand at 3576.620 kb on + strand at 3576.621 kb on - strand at 3576.621 kb on - strand at 3576.638 kb on + strand at 3576.638 kb on + strand at 3576.639 kb on - strand at 3576.639 kb on - strand at 3576.639 kb on - strand at 3576.639 kb on - strand at 3576.700 kb on - strand at 3577.016 kb on - strand, within Pf1N1B4_3195 at 3577.113 kb on + strand, within Pf1N1B4_3195 at 3577.113 kb on + strand, within Pf1N1B4_3195 at 3577.114 kb on - strand, within Pf1N1B4_3195 at 3577.178 kb on + strand at 3577.178 kb on + strand, within Pf1N1B4_3195 at 3577.342 kb on + strand, within Pf1N1B4_3195 at 3577.579 kb on + strand, within Pf1N1B4_3195 at 3577.580 kb on - strand, within Pf1N1B4_3195 at 3577.580 kb on - strand, within Pf1N1B4_3195 at 3577.624 kb on + strand at 3577.624 kb on + strand, within Pf1N1B4_3195 at 3577.624 kb on + strand, within Pf1N1B4_3195 at 3577.625 kb on - strand, within Pf1N1B4_3195 at 3577.625 kb on - strand, within Pf1N1B4_3195 at 3577.687 kb on + strand, within Pf1N1B4_3195 at 3577.687 kb on + strand at 3577.688 kb on - strand, within Pf1N1B4_3195 at 3577.688 kb on - strand at 3577.691 kb on - strand, within Pf1N1B4_3195 at 3577.741 kb on + strand, within Pf1N1B4_3195 at 3578.129 kb on - strand, within Pf1N1B4_3195 at 3578.129 kb on - strand, within Pf1N1B4_3195 at 3578.159 kb on - strand, within Pf1N1B4_3195 at 3578.363 kb on - strand, within Pf1N1B4_3195 at 3578.561 kb on - strand, within Pf1N1B4_3195 at 3578.587 kb on + strand, within Pf1N1B4_3195 at 3578.587 kb on + strand, within Pf1N1B4_3195 at 3578.588 kb on - strand, within Pf1N1B4_3195 at 3578.718 kb on + strand, within Pf1N1B4_3195 at 3578.718 kb on + strand, within Pf1N1B4_3195 at 3578.718 kb on + strand, within Pf1N1B4_3195 at 3578.719 kb on - strand, within Pf1N1B4_3195 at 3578.719 kb on - strand, within Pf1N1B4_3195 at 3578.719 kb on - strand, within Pf1N1B4_3195 at 3578.722 kb on + strand, within Pf1N1B4_3195 at 3578.722 kb on + strand, within Pf1N1B4_3195 at 3578.722 kb on + strand, within Pf1N1B4_3195 at 3578.722 kb on + strand, within Pf1N1B4_3195 at 3578.722 kb on + strand, within Pf1N1B4_3195 at 3578.723 kb on - strand, within Pf1N1B4_3195 at 3578.723 kb on - strand, within Pf1N1B4_3195 at 3578.723 kb on - strand, within Pf1N1B4_3195 at 3578.941 kb on + strand, within Pf1N1B4_3195 at 3579.220 kb on + strand at 3579.221 kb on - strand at 3579.290 kb on + strand at 3579.348 kb on + strand at 3579.349 kb on - strand at 3579.421 kb on + strand at 3579.421 kb on + strand at 3579.421 kb on + strand at 3579.422 kb on - strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.424 kb on - strand at 3579.424 kb on - strand at 3579.424 kb on - strand at 3579.468 kb on + strand at 3579.468 kb on + strand at 3579.469 kb on - strand at 3579.469 kb on - strand at 3579.476 kb on + strand at 3579.476 kb on + strand at 3579.476 kb on + strand at 3579.477 kb on - strand at 3579.477 kb on - strand at 3579.477 kb on - strand at 3579.477 kb on - strand at 3579.477 kb on - strand at 3579.477 kb on - strand at 3579.844 kb on - strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.952 kb on + strand, within Pf1N1B4_3196 at 3579.953 kb on - strand, within Pf1N1B4_3196 at 3579.953 kb on - strand, within Pf1N1B4_3196 at 3580.181 kb on + strand, within Pf1N1B4_3196 at 3580.181 kb on + strand, within Pf1N1B4_3196 at 3580.181 kb on + strand, within Pf1N1B4_3196 at 3580.182 kb on - strand, within Pf1N1B4_3196 at 3580.182 kb on - strand, within Pf1N1B4_3196 at 3580.182 kb on - strand, within Pf1N1B4_3196 at 3580.182 kb on - strand, within Pf1N1B4_3196 at 3580.183 kb on + strand, within Pf1N1B4_3196 at 3580.183 kb on + strand, within Pf1N1B4_3196 at 3580.190 kb on + strand, within Pf1N1B4_3196 at 3580.190 kb on + strand, within Pf1N1B4_3196 at 3580.191 kb on - strand, within Pf1N1B4_3196
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 2.5 mM remove 3,575,887 + Pf1N1B4_3194 0.67 +1.4 3,576,620 + +0.6 3,576,620 + -0.9 3,576,620 + +0.1 3,576,620 + +0.1 3,576,620 + +0.4 3,576,621 - +0.1 3,576,621 - +0.1 3,576,638 + +0.2 3,576,638 + +0.1 3,576,639 - +0.1 3,576,639 - -0.9 3,576,639 - +2.6 3,576,639 - -0.9 3,576,700 - -1.9 3,577,016 - Pf1N1B4_3195 0.13 -0.9 3,577,113 + Pf1N1B4_3195 0.16 -1.5 3,577,113 + Pf1N1B4_3195 0.16 +0.6 3,577,114 - Pf1N1B4_3195 0.16 -0.8 3,577,178 + +0.1 3,577,178 + Pf1N1B4_3195 0.19 -0.5 3,577,342 + Pf1N1B4_3195 0.25 +0.1 3,577,579 + Pf1N1B4_3195 0.35 +1.1 3,577,580 - Pf1N1B4_3195 0.35 -1.5 3,577,580 - Pf1N1B4_3195 0.35 +1.1 3,577,624 + -0.9 3,577,624 + Pf1N1B4_3195 0.37 -1.5 3,577,624 + Pf1N1B4_3195 0.37 -1.9 3,577,625 - Pf1N1B4_3195 0.37 -0.5 3,577,625 - Pf1N1B4_3195 0.37 -2.3 3,577,687 + Pf1N1B4_3195 0.39 -0.4 3,577,687 + +0.1 3,577,688 - Pf1N1B4_3195 0.39 +0.1 3,577,688 - -0.9 3,577,691 - Pf1N1B4_3195 0.39 +0.1 3,577,741 + Pf1N1B4_3195 0.41 +0.1 3,578,129 - Pf1N1B4_3195 0.57 +1.1 3,578,129 - Pf1N1B4_3195 0.57 +2.6 3,578,159 - Pf1N1B4_3195 0.58 -0.9 3,578,363 - Pf1N1B4_3195 0.66 -0.9 3,578,561 - Pf1N1B4_3195 0.74 +1.3 3,578,587 + Pf1N1B4_3195 0.75 -1.5 3,578,587 + Pf1N1B4_3195 0.75 -1.9 3,578,588 - Pf1N1B4_3195 0.75 -2.9 3,578,718 + Pf1N1B4_3195 0.80 +1.6 3,578,718 + Pf1N1B4_3195 0.80 -2.3 3,578,718 + Pf1N1B4_3195 0.80 +0.1 3,578,719 - Pf1N1B4_3195 0.80 -1.5 3,578,719 - Pf1N1B4_3195 0.80 +0.9 3,578,719 - Pf1N1B4_3195 0.80 -0.9 3,578,722 + Pf1N1B4_3195 0.80 +2.4 3,578,722 + Pf1N1B4_3195 0.80 -1.5 3,578,722 + Pf1N1B4_3195 0.80 -0.7 3,578,722 + Pf1N1B4_3195 0.80 -1.9 3,578,722 + Pf1N1B4_3195 0.80 -1.5 3,578,723 - Pf1N1B4_3195 0.80 -2.8 3,578,723 - Pf1N1B4_3195 0.80 +0.1 3,578,723 - Pf1N1B4_3195 0.80 -0.9 3,578,941 + Pf1N1B4_3195 0.89 +0.1 3,579,220 + -0.9 3,579,221 - +1.9 3,579,290 + +0.1 3,579,348 + -1.2 3,579,349 - -0.9 3,579,421 + -0.5 3,579,421 + -0.5 3,579,421 + +2.2 3,579,422 - +0.1 3,579,423 + -0.9 3,579,423 + -0.1 3,579,423 + -1.5 3,579,423 + +0.8 3,579,423 + -1.9 3,579,423 + -1.8 3,579,423 + +0.1 3,579,423 + -0.5 3,579,424 - -1.5 3,579,424 - -0.2 3,579,424 - +1.1 3,579,468 + +0.6 3,579,468 + +0.6 3,579,469 - +0.9 3,579,469 - +2.5 3,579,476 + +0.1 3,579,476 + +0.1 3,579,476 + +1.2 3,579,477 - +1.1 3,579,477 - +0.6 3,579,477 - +0.1 3,579,477 - +0.9 3,579,477 - -0.9 3,579,477 - +1.1 3,579,844 - Pf1N1B4_3196 0.34 -1.5 3,579,930 + Pf1N1B4_3196 0.42 +0.8 3,579,930 + Pf1N1B4_3196 0.42 +1.1 3,579,930 + Pf1N1B4_3196 0.42 +0.1 3,579,930 + Pf1N1B4_3196 0.42 +0.1 3,579,930 + Pf1N1B4_3196 0.42 +1.1 3,579,930 + Pf1N1B4_3196 0.42 +0.5 3,579,931 - Pf1N1B4_3196 0.42 -2.3 3,579,931 - Pf1N1B4_3196 0.42 +0.1 3,579,931 - Pf1N1B4_3196 0.42 -0.9 3,579,931 - Pf1N1B4_3196 0.42 +0.6 3,579,931 - Pf1N1B4_3196 0.42 +0.7 3,579,931 - Pf1N1B4_3196 0.42 +0.9 3,579,952 + Pf1N1B4_3196 0.44 -1.9 3,579,953 - Pf1N1B4_3196 0.44 -0.5 3,579,953 - Pf1N1B4_3196 0.44 +0.5 3,580,181 + Pf1N1B4_3196 0.64 -0.9 3,580,181 + Pf1N1B4_3196 0.64 -0.9 3,580,181 + Pf1N1B4_3196 0.64 +0.1 3,580,182 - Pf1N1B4_3196 0.64 +1.1 3,580,182 - Pf1N1B4_3196 0.64 +0.6 3,580,182 - Pf1N1B4_3196 0.64 -0.9 3,580,182 - Pf1N1B4_3196 0.64 +1.1 3,580,183 + Pf1N1B4_3196 0.64 -1.3 3,580,183 + Pf1N1B4_3196 0.64 -0.9 3,580,190 + Pf1N1B4_3196 0.65 +0.6 3,580,190 + Pf1N1B4_3196 0.65 +0.6 3,580,191 - Pf1N1B4_3196 0.65 +0.1
Or see this region's nucleotide sequence