Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_242

Experiment: Aluminum chloride 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_240 and Pf1N1B4_241 are separated by 163 nucleotidesPf1N1B4_241 and Pf1N1B4_242 are separated by 192 nucleotidesPf1N1B4_242 and Pf1N1B4_243 are separated by 1 nucleotides Pf1N1B4_240 - Transcriptional regulators, at 299,003 to 299,323 _240 Pf1N1B4_241 - Thiol-disulfide isomerase and thioredoxins, at 299,487 to 299,744 _241 Pf1N1B4_242 - Glutathione-regulated potassium-efflux system protein KefB, at 299,937 to 301,649 _242 Pf1N1B4_243 - Ammonium transporter, at 301,651 to 302,925 _243 Position (kb) 299 300 301 302Strain fitness (log2 ratio) -2 -1 0 1 2at 298.953 kb on - strandat 298.990 kb on + strandat 298.990 kb on + strandat 298.991 kb on - strandat 298.991 kb on - strandat 299.103 kb on + strand, within Pf1N1B4_240at 299.103 kb on + strand, within Pf1N1B4_240at 299.103 kb on + strand, within Pf1N1B4_240at 299.189 kb on - strand, within Pf1N1B4_240at 299.357 kb on + strandat 299.411 kb on - strandat 299.576 kb on + strand, within Pf1N1B4_241at 299.587 kb on + strand, within Pf1N1B4_241at 299.750 kb on + strandat 299.750 kb on + strandat 299.750 kb on + strandat 299.751 kb on - strandat 299.751 kb on - strandat 299.763 kb on + strandat 299.764 kb on - strandat 299.798 kb on + strandat 299.900 kb on + strandat 299.900 kb on + strandat 299.900 kb on + strandat 299.900 kb on + strandat 299.900 kb on + strandat 299.901 kb on - strandat 299.901 kb on - strandat 299.901 kb on - strandat 300.409 kb on + strand, within Pf1N1B4_242at 300.456 kb on + strand, within Pf1N1B4_242at 300.677 kb on - strand, within Pf1N1B4_242at 300.677 kb on - strand, within Pf1N1B4_242at 301.207 kb on + strand, within Pf1N1B4_242at 301.207 kb on + strand, within Pf1N1B4_242at 301.207 kb on + strand, within Pf1N1B4_242at 301.208 kb on - strand, within Pf1N1B4_242at 301.218 kb on + strand, within Pf1N1B4_242at 301.218 kb on + strand, within Pf1N1B4_242at 301.218 kb on + strand, within Pf1N1B4_242at 301.219 kb on - strand, within Pf1N1B4_242at 301.342 kb on + strand, within Pf1N1B4_242at 301.384 kb on + strand, within Pf1N1B4_242at 301.621 kb on - strandat 301.703 kb on + strandat 301.704 kb on - strandat 301.704 kb on - strandat 301.704 kb on - strandat 302.188 kb on + strand, within Pf1N1B4_243at 302.456 kb on + strand, within Pf1N1B4_243at 302.456 kb on + strand, within Pf1N1B4_243at 302.456 kb on + strand, within Pf1N1B4_243at 302.457 kb on - strand, within Pf1N1B4_243at 302.457 kb on - strand, within Pf1N1B4_243at 302.588 kb on + strand, within Pf1N1B4_243

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Per-strain Table

Position Strand Gene LocusTag Fraction Aluminum chloride 2.5 mM
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298,953 - -0.2
298,990 + +0.4
298,990 + -2.2
298,991 - +0.5
298,991 - -1.8
299,103 + Pf1N1B4_240 0.31 -1.2
299,103 + Pf1N1B4_240 0.31 +1.4
299,103 + Pf1N1B4_240 0.31 +0.1
299,189 - Pf1N1B4_240 0.58 +0.5
299,357 + -1.2
299,411 - -0.8
299,576 + Pf1N1B4_241 0.34 +0.3
299,587 + Pf1N1B4_241 0.39 +0.8
299,750 + +1.0
299,750 + -0.0
299,750 + +0.1
299,751 - +0.4
299,751 - +0.8
299,763 + -0.2
299,764 - -1.0
299,798 + -0.2
299,900 + +0.3
299,900 + +0.8
299,900 + +1.0
299,900 + +1.1
299,900 + -0.2
299,901 - +0.7
299,901 - -0.2
299,901 - +0.0
300,409 + Pf1N1B4_242 0.28 +0.5
300,456 + Pf1N1B4_242 0.30 -0.6
300,677 - Pf1N1B4_242 0.43 -1.2
300,677 - Pf1N1B4_242 0.43 -1.4
301,207 + Pf1N1B4_242 0.74 -1.8
301,207 + Pf1N1B4_242 0.74 +0.2
301,207 + Pf1N1B4_242 0.74 -1.8
301,208 - Pf1N1B4_242 0.74 +0.2
301,218 + Pf1N1B4_242 0.75 -0.2
301,218 + Pf1N1B4_242 0.75 -1.6
301,218 + Pf1N1B4_242 0.75 -0.6
301,219 - Pf1N1B4_242 0.75 -2.5
301,342 + Pf1N1B4_242 0.82 -2.5
301,384 + Pf1N1B4_242 0.84 -0.2
301,621 - +0.5
301,703 + -1.8
301,704 - +0.8
301,704 - -0.6
301,704 - +1.8
302,188 + Pf1N1B4_243 0.42 -0.2
302,456 + Pf1N1B4_243 0.63 +2.1
302,456 + Pf1N1B4_243 0.63 +0.1
302,456 + Pf1N1B4_243 0.63 +0.0
302,457 - Pf1N1B4_243 0.63 -1.2
302,457 - Pf1N1B4_243 0.63 +0.6
302,588 + Pf1N1B4_243 0.73 +0.6

Or see this region's nucleotide sequence