Experiment: Aluminum chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2364 and Pf1N1B4_2365 overlap by 4 nucleotides Pf1N1B4_2365 and Pf1N1B4_2366 overlap by 4 nucleotides Pf1N1B4_2366 and Pf1N1B4_2367 overlap by 4 nucleotides Pf1N1B4_2367 and Pf1N1B4_2368 are separated by 66 nucleotides
Pf1N1B4_2364 - Malonate decarboxylase beta subunit, at 2,681,378 to 2,682,229
_2364
Pf1N1B4_2365 - Malonate decarboxylase gamma subunit, at 2,682,226 to 2,683,029
_2365
Pf1N1B4_2366 - Phosphoribosyl-dephospho-CoA transferase (EC 2.7.7.-), at 2,683,026 to 2,683,643
_2366
Pf1N1B4_2367 - Malonyl CoA acyl carrier protein transacylase (EC 2.3.1.39), at 2,683,640 to 2,684,551
_2367
Pf1N1B4_2368 - Malonate transporter, MadL subunit, at 2,684,618 to 2,685,034
_2368
Position (kb)
2683
2684 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2682.047 kb on + strand, within Pf1N1B4_2364 at 2682.047 kb on + strand, within Pf1N1B4_2364 at 2682.235 kb on + strand at 2682.235 kb on + strand at 2682.235 kb on + strand at 2682.329 kb on + strand, within Pf1N1B4_2365 at 2682.355 kb on - strand, within Pf1N1B4_2365 at 2682.526 kb on + strand, within Pf1N1B4_2365 at 2682.527 kb on - strand, within Pf1N1B4_2365 at 2682.586 kb on + strand, within Pf1N1B4_2365 at 2682.586 kb on + strand, within Pf1N1B4_2365 at 2682.586 kb on + strand, within Pf1N1B4_2365 at 2682.586 kb on + strand, within Pf1N1B4_2365 at 2682.586 kb on + strand, within Pf1N1B4_2365 at 2682.587 kb on - strand, within Pf1N1B4_2365 at 2682.587 kb on - strand, within Pf1N1B4_2365 at 2682.587 kb on - strand, within Pf1N1B4_2365 at 2682.587 kb on - strand, within Pf1N1B4_2365 at 2682.808 kb on + strand, within Pf1N1B4_2365 at 2682.808 kb on + strand, within Pf1N1B4_2365 at 2682.808 kb on + strand, within Pf1N1B4_2365 at 2682.809 kb on - strand, within Pf1N1B4_2365 at 2683.246 kb on + strand, within Pf1N1B4_2366 at 2683.246 kb on + strand, within Pf1N1B4_2366 at 2683.527 kb on + strand, within Pf1N1B4_2366 at 2683.537 kb on + strand, within Pf1N1B4_2366 at 2683.537 kb on + strand, within Pf1N1B4_2366 at 2683.537 kb on + strand, within Pf1N1B4_2366 at 2683.538 kb on - strand, within Pf1N1B4_2366 at 2683.538 kb on - strand, within Pf1N1B4_2366 at 2683.538 kb on - strand, within Pf1N1B4_2366 at 2683.538 kb on - strand, within Pf1N1B4_2366 at 2683.800 kb on + strand, within Pf1N1B4_2367 at 2683.800 kb on + strand, within Pf1N1B4_2367 at 2683.800 kb on + strand, within Pf1N1B4_2367 at 2683.800 kb on + strand at 2683.801 kb on - strand, within Pf1N1B4_2367 at 2683.856 kb on + strand, within Pf1N1B4_2367 at 2683.857 kb on - strand, within Pf1N1B4_2367 at 2683.982 kb on + strand, within Pf1N1B4_2367 at 2683.982 kb on + strand, within Pf1N1B4_2367 at 2683.983 kb on - strand, within Pf1N1B4_2367 at 2684.078 kb on - strand, within Pf1N1B4_2367 at 2684.309 kb on - strand, within Pf1N1B4_2367 at 2684.379 kb on + strand, within Pf1N1B4_2367 at 2684.403 kb on + strand, within Pf1N1B4_2367 at 2684.404 kb on - strand, within Pf1N1B4_2367 at 2684.404 kb on - strand, within Pf1N1B4_2367 at 2684.409 kb on + strand, within Pf1N1B4_2367 at 2684.410 kb on - strand, within Pf1N1B4_2367 at 2684.410 kb on - strand, within Pf1N1B4_2367 at 2684.410 kb on - strand, within Pf1N1B4_2367 at 2684.506 kb on + strand at 2684.506 kb on + strand at 2684.507 kb on - strand at 2684.549 kb on + strand at 2684.549 kb on + strand at 2684.549 kb on + strand at 2684.549 kb on + strand at 2684.549 kb on + strand at 2684.550 kb on - strand at 2684.574 kb on + strand at 2684.575 kb on - strand at 2684.611 kb on + strand at 2684.611 kb on + strand at 2684.611 kb on + strand at 2684.611 kb on + strand at 2684.611 kb on + strand at 2684.612 kb on - strand at 2684.623 kb on + strand at 2684.623 kb on + strand at 2684.623 kb on + strand at 2684.624 kb on - strand at 2684.624 kb on - strand at 2684.624 kb on - strand at 2684.624 kb on - strand at 2684.627 kb on + strand at 2684.627 kb on + strand at 2684.627 kb on + strand at 2684.628 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 2.5 mM remove 2,682,047 + Pf1N1B4_2364 0.79 +0.9 2,682,047 + Pf1N1B4_2364 0.79 +0.4 2,682,235 + +0.4 2,682,235 + +1.9 2,682,235 + +1.0 2,682,329 + Pf1N1B4_2365 0.13 -3.2 2,682,355 - Pf1N1B4_2365 0.16 +1.7 2,682,526 + Pf1N1B4_2365 0.37 -0.6 2,682,527 - Pf1N1B4_2365 0.37 -0.1 2,682,586 + Pf1N1B4_2365 0.45 -2.0 2,682,586 + Pf1N1B4_2365 0.45 -2.0 2,682,586 + Pf1N1B4_2365 0.45 -0.1 2,682,586 + Pf1N1B4_2365 0.45 +0.1 2,682,586 + Pf1N1B4_2365 0.45 +0.3 2,682,587 - Pf1N1B4_2365 0.45 -0.2 2,682,587 - Pf1N1B4_2365 0.45 -0.5 2,682,587 - Pf1N1B4_2365 0.45 +0.4 2,682,587 - Pf1N1B4_2365 0.45 +1.2 2,682,808 + Pf1N1B4_2365 0.72 -2.2 2,682,808 + Pf1N1B4_2365 0.72 +2.4 2,682,808 + Pf1N1B4_2365 0.72 +1.1 2,682,809 - Pf1N1B4_2365 0.73 -1.6 2,683,246 + Pf1N1B4_2366 0.36 -2.5 2,683,246 + Pf1N1B4_2366 0.36 +0.4 2,683,527 + Pf1N1B4_2366 0.81 +0.4 2,683,537 + Pf1N1B4_2366 0.83 +1.9 2,683,537 + Pf1N1B4_2366 0.83 -0.2 2,683,537 + Pf1N1B4_2366 0.83 -0.6 2,683,538 - Pf1N1B4_2366 0.83 -1.2 2,683,538 - Pf1N1B4_2366 0.83 -0.6 2,683,538 - Pf1N1B4_2366 0.83 -1.2 2,683,538 - Pf1N1B4_2366 0.83 -0.6 2,683,800 + Pf1N1B4_2367 0.18 -1.5 2,683,800 + Pf1N1B4_2367 0.18 -0.5 2,683,800 + Pf1N1B4_2367 0.18 -1.0 2,683,800 + -0.7 2,683,801 - Pf1N1B4_2367 0.18 -0.7 2,683,856 + Pf1N1B4_2367 0.24 -0.2 2,683,857 - Pf1N1B4_2367 0.24 +0.1 2,683,982 + Pf1N1B4_2367 0.38 -1.5 2,683,982 + Pf1N1B4_2367 0.38 +0.9 2,683,983 - Pf1N1B4_2367 0.38 +0.0 2,684,078 - Pf1N1B4_2367 0.48 +0.3 2,684,309 - Pf1N1B4_2367 0.73 +0.6 2,684,379 + Pf1N1B4_2367 0.81 +0.8 2,684,403 + Pf1N1B4_2367 0.84 +0.3 2,684,404 - Pf1N1B4_2367 0.84 +0.3 2,684,404 - Pf1N1B4_2367 0.84 +0.3 2,684,409 + Pf1N1B4_2367 0.84 +0.6 2,684,410 - Pf1N1B4_2367 0.84 -0.4 2,684,410 - Pf1N1B4_2367 0.84 +0.7 2,684,410 - Pf1N1B4_2367 0.84 -0.0 2,684,506 + +1.3 2,684,506 + -1.2 2,684,507 - -2.2 2,684,549 + +0.7 2,684,549 + +1.1 2,684,549 + -1.2 2,684,549 + -2.8 2,684,549 + -1.7 2,684,550 - -2.2 2,684,574 + +0.5 2,684,575 - -1.1 2,684,611 + -0.5 2,684,611 + -1.7 2,684,611 + -1.1 2,684,611 + -1.2 2,684,611 + -0.7 2,684,612 - +0.8 2,684,623 + -1.0 2,684,623 + +0.3 2,684,623 + +1.7 2,684,624 - -2.5 2,684,624 - +0.6 2,684,624 - -0.2 2,684,624 - +1.3 2,684,627 + -0.8 2,684,627 + +1.2 2,684,627 + +0.3 2,684,628 - +1.3
Or see this region's nucleotide sequence