Experiment: Aluminum chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1743 and Pf1N1B4_1744 are separated by 42 nucleotides Pf1N1B4_1744 and Pf1N1B4_1745 are separated by 12 nucleotides
Pf1N1B4_1743 - Biofilm PGA synthesis N-glycosyltransferase PgaC (EC 2.4.-.-), at 1,992,063 to 1,993,418
_1743
Pf1N1B4_1744 - Biofilm PGA synthesis deacetylase PgaB (EC 3.-), at 1,993,461 to 1,995,416
_1744
Pf1N1B4_1745 - Biofilm PGA outer membrane secretin PgaA, at 1,995,429 to 1,997,909
_1745
Position (kb)
1993
1994
1995
1996 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1992.895 kb on + strand, within Pf1N1B4_1743 at 1992.896 kb on - strand, within Pf1N1B4_1743 at 1992.896 kb on - strand, within Pf1N1B4_1743 at 1993.168 kb on + strand, within Pf1N1B4_1743 at 1993.168 kb on + strand, within Pf1N1B4_1743 at 1993.168 kb on + strand, within Pf1N1B4_1743 at 1993.168 kb on + strand, within Pf1N1B4_1743 at 1993.168 kb on + strand, within Pf1N1B4_1743 at 1993.169 kb on - strand, within Pf1N1B4_1743 at 1993.169 kb on - strand, within Pf1N1B4_1743 at 1993.169 kb on - strand, within Pf1N1B4_1743 at 1993.169 kb on - strand, within Pf1N1B4_1743 at 1993.223 kb on + strand, within Pf1N1B4_1743 at 1993.224 kb on - strand, within Pf1N1B4_1743 at 1993.224 kb on - strand, within Pf1N1B4_1743 at 1993.224 kb on - strand, within Pf1N1B4_1743 at 1993.267 kb on + strand, within Pf1N1B4_1743 at 1993.268 kb on - strand, within Pf1N1B4_1743 at 1993.401 kb on + strand at 1993.402 kb on - strand at 1993.421 kb on + strand at 1993.421 kb on + strand at 1993.500 kb on - strand at 1993.737 kb on - strand, within Pf1N1B4_1744 at 1993.827 kb on + strand, within Pf1N1B4_1744 at 1993.827 kb on + strand, within Pf1N1B4_1744 at 1993.827 kb on + strand, within Pf1N1B4_1744 at 1993.828 kb on - strand, within Pf1N1B4_1744 at 1993.975 kb on + strand, within Pf1N1B4_1744 at 1994.036 kb on - strand, within Pf1N1B4_1744 at 1994.036 kb on - strand, within Pf1N1B4_1744 at 1994.077 kb on + strand, within Pf1N1B4_1744 at 1994.077 kb on + strand, within Pf1N1B4_1744 at 1994.131 kb on + strand, within Pf1N1B4_1744 at 1994.131 kb on + strand, within Pf1N1B4_1744 at 1994.132 kb on - strand, within Pf1N1B4_1744 at 1994.233 kb on + strand, within Pf1N1B4_1744 at 1994.233 kb on + strand, within Pf1N1B4_1744 at 1994.233 kb on + strand, within Pf1N1B4_1744 at 1994.285 kb on + strand, within Pf1N1B4_1744 at 1994.320 kb on + strand, within Pf1N1B4_1744 at 1994.321 kb on - strand, within Pf1N1B4_1744 at 1994.321 kb on - strand, within Pf1N1B4_1744 at 1994.321 kb on - strand, within Pf1N1B4_1744 at 1994.698 kb on + strand, within Pf1N1B4_1744 at 1994.779 kb on + strand, within Pf1N1B4_1744 at 1994.958 kb on - strand, within Pf1N1B4_1744 at 1995.032 kb on + strand, within Pf1N1B4_1744 at 1995.032 kb on + strand, within Pf1N1B4_1744 at 1995.032 kb on + strand, within Pf1N1B4_1744 at 1995.032 kb on + strand, within Pf1N1B4_1744 at 1995.033 kb on - strand, within Pf1N1B4_1744 at 1995.033 kb on - strand, within Pf1N1B4_1744 at 1995.034 kb on + strand, within Pf1N1B4_1744 at 1995.035 kb on - strand, within Pf1N1B4_1744 at 1995.064 kb on + strand, within Pf1N1B4_1744 at 1995.064 kb on + strand, within Pf1N1B4_1744 at 1995.065 kb on - strand, within Pf1N1B4_1744 at 1995.065 kb on - strand, within Pf1N1B4_1744 at 1995.065 kb on - strand, within Pf1N1B4_1744 at 1995.065 kb on - strand, within Pf1N1B4_1744 at 1995.065 kb on - strand, within Pf1N1B4_1744 at 1995.065 kb on - strand, within Pf1N1B4_1744 at 1995.065 kb on - strand, within Pf1N1B4_1744 at 1995.065 kb on - strand, within Pf1N1B4_1744 at 1995.169 kb on + strand, within Pf1N1B4_1744 at 1995.169 kb on + strand, within Pf1N1B4_1744 at 1995.169 kb on + strand, within Pf1N1B4_1744 at 1995.169 kb on + strand, within Pf1N1B4_1744 at 1995.169 kb on + strand, within Pf1N1B4_1744 at 1995.169 kb on + strand, within Pf1N1B4_1744 at 1995.169 kb on + strand, within Pf1N1B4_1744 at 1995.170 kb on - strand, within Pf1N1B4_1744 at 1995.170 kb on - strand, within Pf1N1B4_1744 at 1995.170 kb on - strand, within Pf1N1B4_1744 at 1995.170 kb on - strand, within Pf1N1B4_1744 at 1995.170 kb on - strand, within Pf1N1B4_1744 at 1995.170 kb on - strand, within Pf1N1B4_1744 at 1995.279 kb on + strand at 1995.280 kb on - strand at 1995.280 kb on - strand at 1995.280 kb on - strand at 1995.407 kb on + strand at 1995.407 kb on + strand at 1995.407 kb on + strand at 1995.408 kb on - strand at 1995.408 kb on - strand at 1995.408 kb on - strand at 1995.408 kb on - strand at 1995.430 kb on + strand at 1995.431 kb on - strand at 1995.439 kb on + strand at 1995.439 kb on + strand at 1995.440 kb on - strand at 1995.440 kb on - strand at 1995.440 kb on - strand at 1995.487 kb on + strand at 1995.528 kb on + strand at 1995.528 kb on + strand at 1995.528 kb on + strand at 1995.528 kb on + strand at 1995.528 kb on + strand at 1995.528 kb on + strand at 1995.529 kb on - strand at 1995.529 kb on - strand at 1995.599 kb on + strand at 1995.601 kb on + strand at 1995.601 kb on + strand at 1995.601 kb on + strand at 1995.601 kb on + strand at 1995.601 kb on + strand at 1995.601 kb on + strand at 1995.601 kb on + strand at 1995.601 kb on + strand at 1995.601 kb on + strand at 1995.601 kb on + strand at 1995.602 kb on - strand at 1995.602 kb on - strand at 1995.602 kb on - strand at 1995.602 kb on - strand at 1995.602 kb on - strand at 1995.602 kb on - strand at 1995.602 kb on - strand at 1995.602 kb on - strand at 1995.646 kb on + strand at 1995.656 kb on - strand at 1995.741 kb on - strand, within Pf1N1B4_1745 at 1995.741 kb on - strand, within Pf1N1B4_1745 at 1995.741 kb on - strand, within Pf1N1B4_1745 at 1995.973 kb on + strand, within Pf1N1B4_1745 at 1995.974 kb on - strand at 1995.974 kb on - strand, within Pf1N1B4_1745 at 1996.058 kb on - strand, within Pf1N1B4_1745 at 1996.058 kb on - strand, within Pf1N1B4_1745 at 1996.110 kb on + strand, within Pf1N1B4_1745 at 1996.206 kb on - strand, within Pf1N1B4_1745 at 1996.216 kb on + strand, within Pf1N1B4_1745 at 1996.216 kb on + strand, within Pf1N1B4_1745 at 1996.217 kb on - strand, within Pf1N1B4_1745 at 1996.217 kb on - strand, within Pf1N1B4_1745 at 1996.217 kb on - strand, within Pf1N1B4_1745 at 1996.285 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 2.5 mM remove 1,992,895 + Pf1N1B4_1743 0.61 -0.9 1,992,896 - Pf1N1B4_1743 0.61 +0.0 1,992,896 - Pf1N1B4_1743 0.61 -2.6 1,993,168 + Pf1N1B4_1743 0.81 +0.9 1,993,168 + Pf1N1B4_1743 0.81 +0.4 1,993,168 + Pf1N1B4_1743 0.81 +0.7 1,993,168 + Pf1N1B4_1743 0.81 +0.0 1,993,168 + Pf1N1B4_1743 0.81 +0.4 1,993,169 - Pf1N1B4_1743 0.82 -0.6 1,993,169 - Pf1N1B4_1743 0.82 -0.6 1,993,169 - Pf1N1B4_1743 0.82 +0.1 1,993,169 - Pf1N1B4_1743 0.82 +0.9 1,993,223 + Pf1N1B4_1743 0.86 -0.2 1,993,224 - Pf1N1B4_1743 0.86 +0.2 1,993,224 - Pf1N1B4_1743 0.86 -0.6 1,993,224 - Pf1N1B4_1743 0.86 +0.4 1,993,267 + Pf1N1B4_1743 0.89 -2.2 1,993,268 - Pf1N1B4_1743 0.89 +0.9 1,993,401 + +0.8 1,993,402 - +0.4 1,993,421 + +0.1 1,993,421 + +0.1 1,993,500 - -1.6 1,993,737 - Pf1N1B4_1744 0.14 -1.2 1,993,827 + Pf1N1B4_1744 0.19 +0.4 1,993,827 + Pf1N1B4_1744 0.19 -0.6 1,993,827 + Pf1N1B4_1744 0.19 -1.5 1,993,828 - Pf1N1B4_1744 0.19 +0.1 1,993,975 + Pf1N1B4_1744 0.26 -1.9 1,994,036 - Pf1N1B4_1744 0.29 +0.4 1,994,036 - Pf1N1B4_1744 0.29 -0.2 1,994,077 + Pf1N1B4_1744 0.31 -0.3 1,994,077 + Pf1N1B4_1744 0.31 -0.1 1,994,131 + Pf1N1B4_1744 0.34 -1.2 1,994,131 + Pf1N1B4_1744 0.34 +0.0 1,994,132 - Pf1N1B4_1744 0.34 +0.4 1,994,233 + Pf1N1B4_1744 0.39 +1.4 1,994,233 + Pf1N1B4_1744 0.39 +0.0 1,994,233 + Pf1N1B4_1744 0.39 -0.2 1,994,285 + Pf1N1B4_1744 0.42 +1.1 1,994,320 + Pf1N1B4_1744 0.44 +0.4 1,994,321 - Pf1N1B4_1744 0.44 -1.2 1,994,321 - Pf1N1B4_1744 0.44 +0.4 1,994,321 - Pf1N1B4_1744 0.44 -1.0 1,994,698 + Pf1N1B4_1744 0.63 +1.4 1,994,779 + Pf1N1B4_1744 0.67 +0.4 1,994,958 - Pf1N1B4_1744 0.77 -1.6 1,995,032 + Pf1N1B4_1744 0.80 -2.4 1,995,032 + Pf1N1B4_1744 0.80 +1.7 1,995,032 + Pf1N1B4_1744 0.80 +0.4 1,995,032 + Pf1N1B4_1744 0.80 +0.2 1,995,033 - Pf1N1B4_1744 0.80 +0.7 1,995,033 - Pf1N1B4_1744 0.80 -0.9 1,995,034 + Pf1N1B4_1744 0.80 +1.3 1,995,035 - Pf1N1B4_1744 0.80 -1.2 1,995,064 + Pf1N1B4_1744 0.82 +0.1 1,995,064 + Pf1N1B4_1744 0.82 -1.0 1,995,065 - Pf1N1B4_1744 0.82 +1.0 1,995,065 - Pf1N1B4_1744 0.82 -1.2 1,995,065 - Pf1N1B4_1744 0.82 +2.3 1,995,065 - Pf1N1B4_1744 0.82 +0.3 1,995,065 - Pf1N1B4_1744 0.82 +0.0 1,995,065 - Pf1N1B4_1744 0.82 +0.7 1,995,065 - Pf1N1B4_1744 0.82 +0.4 1,995,065 - Pf1N1B4_1744 0.82 -0.8 1,995,169 + Pf1N1B4_1744 0.87 +1.4 1,995,169 + Pf1N1B4_1744 0.87 -0.3 1,995,169 + Pf1N1B4_1744 0.87 +1.0 1,995,169 + Pf1N1B4_1744 0.87 +0.9 1,995,169 + Pf1N1B4_1744 0.87 -2.2 1,995,169 + Pf1N1B4_1744 0.87 +0.6 1,995,169 + Pf1N1B4_1744 0.87 +1.4 1,995,170 - Pf1N1B4_1744 0.87 +2.0 1,995,170 - Pf1N1B4_1744 0.87 +0.6 1,995,170 - Pf1N1B4_1744 0.87 +0.0 1,995,170 - Pf1N1B4_1744 0.87 -0.4 1,995,170 - Pf1N1B4_1744 0.87 +0.8 1,995,170 - Pf1N1B4_1744 0.87 -0.6 1,995,279 + +0.4 1,995,280 - +2.4 1,995,280 - +1.0 1,995,280 - -1.2 1,995,407 + +2.3 1,995,407 + +0.0 1,995,407 + -0.1 1,995,408 - +1.0 1,995,408 - +0.1 1,995,408 - -0.7 1,995,408 - -0.9 1,995,430 + -1.2 1,995,431 - -2.4 1,995,439 + +0.4 1,995,439 + -0.0 1,995,440 - +0.7 1,995,440 - -0.6 1,995,440 - +1.1 1,995,487 + -0.2 1,995,528 + -1.9 1,995,528 + +0.4 1,995,528 + +0.4 1,995,528 + +3.6 1,995,528 + +1.9 1,995,528 + +1.7 1,995,529 - +0.8 1,995,529 - +0.4 1,995,599 + -0.2 1,995,601 + -1.2 1,995,601 + -1.2 1,995,601 + -0.1 1,995,601 + -1.1 1,995,601 + +0.5 1,995,601 + +0.3 1,995,601 + -1.6 1,995,601 + -1.2 1,995,601 + -0.6 1,995,601 + -1.2 1,995,602 - -3.2 1,995,602 - +0.6 1,995,602 - +1.1 1,995,602 - +1.4 1,995,602 - -0.2 1,995,602 - -0.2 1,995,602 - +0.4 1,995,602 - +0.4 1,995,646 + +0.4 1,995,656 - -0.3 1,995,741 - Pf1N1B4_1745 0.13 -0.3 1,995,741 - Pf1N1B4_1745 0.13 -1.2 1,995,741 - Pf1N1B4_1745 0.13 -0.9 1,995,973 + Pf1N1B4_1745 0.22 +0.4 1,995,974 - -1.2 1,995,974 - Pf1N1B4_1745 0.22 +0.7 1,996,058 - Pf1N1B4_1745 0.25 +1.1 1,996,058 - Pf1N1B4_1745 0.25 +0.5 1,996,110 + Pf1N1B4_1745 0.27 -1.2 1,996,206 - Pf1N1B4_1745 0.31 +0.4 1,996,216 + Pf1N1B4_1745 0.32 +0.4 1,996,216 + Pf1N1B4_1745 0.32 -1.2 1,996,217 - Pf1N1B4_1745 0.32 -2.4 1,996,217 - Pf1N1B4_1745 0.32 +0.5 1,996,217 - Pf1N1B4_1745 0.32 -0.2 1,996,285 + +1.0
Or see this region's nucleotide sequence