Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_16

Experiment: Aluminum chloride 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_15 and Pf1N1B4_16 are separated by 158 nucleotidesPf1N1B4_16 and Pf1N1B4_17 are separated by 105 nucleotides Pf1N1B4_15 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 16,025 to 17,443 _15 Pf1N1B4_16 - Cys regulon transcriptional activator CysB, at 17,602 to 18,492 _16 Pf1N1B4_17 - Sensory box/GGDEF family protein, at 18,598 to 21,855 _17 Position (kb) 17 18 19Strain fitness (log2 ratio) -2 -1 0 1 2 3at 16.704 kb on + strand, within Pf1N1B4_15at 16.704 kb on + strand, within Pf1N1B4_15at 16.704 kb on + strand, within Pf1N1B4_15at 16.774 kb on + strand, within Pf1N1B4_15at 16.775 kb on - strand, within Pf1N1B4_15at 17.165 kb on - strand, within Pf1N1B4_15at 17.364 kb on + strandat 17.365 kb on - strandat 17.365 kb on - strandat 17.365 kb on - strandat 17.365 kb on - strandat 17.365 kb on - strandat 17.471 kb on - strandat 17.823 kb on - strand, within Pf1N1B4_16at 17.927 kb on + strand, within Pf1N1B4_16at 17.931 kb on - strand, within Pf1N1B4_16at 18.271 kb on + strand, within Pf1N1B4_16at 18.272 kb on - strandat 18.392 kb on + strand, within Pf1N1B4_16at 18.392 kb on + strand, within Pf1N1B4_16at 18.392 kb on + strand, within Pf1N1B4_16at 18.398 kb on - strandat 18.398 kb on - strand, within Pf1N1B4_16at 18.521 kb on + strandat 18.522 kb on - strandat 18.524 kb on - strandat 18.597 kb on + strandat 18.598 kb on - strandat 18.598 kb on - strandat 18.709 kb on + strandat 18.805 kb on + strandat 18.805 kb on + strandat 19.136 kb on - strand, within Pf1N1B4_17at 19.136 kb on - strand, within Pf1N1B4_17at 19.355 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Aluminum chloride 2.5 mM
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16,704 + Pf1N1B4_15 0.48 -1.8
16,704 + Pf1N1B4_15 0.48 -1.6
16,704 + Pf1N1B4_15 0.48 +0.7
16,774 + Pf1N1B4_15 0.53 +0.3
16,775 - Pf1N1B4_15 0.53 -0.8
17,165 - Pf1N1B4_15 0.80 +0.4
17,364 + -0.3
17,365 - -0.3
17,365 - +0.3
17,365 - +1.7
17,365 - +3.2
17,365 - +1.3
17,471 - -0.3
17,823 - Pf1N1B4_16 0.25 -0.3
17,927 + Pf1N1B4_16 0.36 -0.3
17,931 - Pf1N1B4_16 0.37 -1.3
18,271 + Pf1N1B4_16 0.75 -0.3
18,272 - -0.3
18,392 + Pf1N1B4_16 0.89 -0.7
18,392 + Pf1N1B4_16 0.89 +1.1
18,392 + Pf1N1B4_16 0.89 -2.3
18,398 - -0.3
18,398 - Pf1N1B4_16 0.89 -0.9
18,521 + -0.3
18,522 - -1.0
18,524 - -0.2
18,597 + +1.1
18,598 - -1.8
18,598 - -0.3
18,709 + +2.3
18,805 + +0.0
18,805 + +0.6
19,136 - Pf1N1B4_17 0.17 -1.8
19,136 - Pf1N1B4_17 0.17 +0.4
19,355 - -0.3

Or see this region's nucleotide sequence