Experiment: Cephalothin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_373 and Ac3H11_374 are separated by 45 nucleotides Ac3H11_374 and Ac3H11_375 are separated by 70 nucleotides Ac3H11_375 and Ac3H11_376 are separated by 301 nucleotides Ac3H11_376 and Ac3H11_564 are separated by 16 nucleotides Ac3H11_564 and Ac3H11_377 are separated by 63 nucleotides
Ac3H11_373 - Excinuclease ABC subunit C, at 47,900 to 49,921
_373
Ac3H11_374 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5), at 49,967 to 50,533
_374
Ac3H11_375 - Tartrate dehydrogenase (EC 1.1.1.93) @ Tartrate decarboxylase (EC 4.1.1.73) @ D-malic enzyme (EC 1.1.1.83), at 50,604 to 51,698
_375
Ac3H11_376 - DNA-binding protein HU-beta, at 52,000 to 52,272
_376
Ac3H11_564 - tRNA-Val-TAC, at 52,289 to 52,361
_564
Ac3H11_377 - Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8), at 52,425 to 54,353
_377
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 49.643 kb on + strand, within Ac3H11_373 at 49.904 kb on + strand at 49.906 kb on + strand at 49.919 kb on + strand at 49.919 kb on + strand at 49.919 kb on + strand at 49.919 kb on + strand at 49.920 kb on - strand at 49.920 kb on - strand at 49.920 kb on - strand at 49.920 kb on - strand at 49.920 kb on - strand at 50.579 kb on + strand at 50.748 kb on + strand, within Ac3H11_375 at 50.748 kb on + strand, within Ac3H11_375 at 50.749 kb on - strand, within Ac3H11_375 at 50.751 kb on + strand, within Ac3H11_375 at 50.752 kb on - strand, within Ac3H11_375 at 50.817 kb on + strand, within Ac3H11_375 at 50.817 kb on + strand, within Ac3H11_375 at 50.817 kb on + strand, within Ac3H11_375 at 50.817 kb on + strand, within Ac3H11_375 at 50.818 kb on - strand, within Ac3H11_375 at 50.818 kb on - strand, within Ac3H11_375 at 50.818 kb on - strand, within Ac3H11_375 at 50.818 kb on - strand, within Ac3H11_375 at 50.818 kb on - strand, within Ac3H11_375 at 50.818 kb on - strand, within Ac3H11_375 at 50.907 kb on + strand, within Ac3H11_375 at 50.907 kb on + strand, within Ac3H11_375 at 50.907 kb on + strand, within Ac3H11_375 at 50.907 kb on + strand, within Ac3H11_375 at 50.908 kb on - strand, within Ac3H11_375 at 50.908 kb on - strand, within Ac3H11_375 at 50.908 kb on - strand, within Ac3H11_375 at 51.034 kb on - strand, within Ac3H11_375 at 51.057 kb on + strand, within Ac3H11_375 at 51.057 kb on + strand, within Ac3H11_375 at 51.057 kb on + strand, within Ac3H11_375 at 51.057 kb on + strand, within Ac3H11_375 at 51.057 kb on + strand, within Ac3H11_375 at 51.057 kb on + strand, within Ac3H11_375 at 51.057 kb on + strand, within Ac3H11_375 at 51.057 kb on + strand, within Ac3H11_375 at 51.058 kb on - strand, within Ac3H11_375 at 51.058 kb on - strand, within Ac3H11_375 at 51.058 kb on - strand, within Ac3H11_375 at 51.058 kb on - strand, within Ac3H11_375 at 51.058 kb on - strand, within Ac3H11_375 at 51.253 kb on - strand, within Ac3H11_375 at 51.739 kb on + strand at 51.739 kb on + strand at 51.790 kb on + strand at 51.791 kb on - strand at 51.796 kb on + strand at 51.834 kb on + strand at 51.834 kb on + strand at 51.834 kb on + strand at 51.889 kb on - strand at 51.889 kb on - strand at 52.191 kb on + strand, within Ac3H11_376
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.005 mg/ml remove 49,643 + Ac3H11_373 0.86 -1.3 49,904 + -0.6 49,906 + -0.7 49,919 + +0.4 49,919 + +0.1 49,919 + +0.3 49,919 + +0.4 49,920 - +0.4 49,920 - +0.9 49,920 - -0.3 49,920 - +0.8 49,920 - +0.6 50,579 + -1.3 50,748 + Ac3H11_375 0.13 +0.0 50,748 + Ac3H11_375 0.13 -1.5 50,749 - Ac3H11_375 0.13 +2.2 50,751 + Ac3H11_375 0.13 +1.2 50,752 - Ac3H11_375 0.14 +0.0 50,817 + Ac3H11_375 0.19 +0.4 50,817 + Ac3H11_375 0.19 +0.6 50,817 + Ac3H11_375 0.19 +0.2 50,817 + Ac3H11_375 0.19 -0.6 50,818 - Ac3H11_375 0.20 -0.1 50,818 - Ac3H11_375 0.20 -0.0 50,818 - Ac3H11_375 0.20 -0.1 50,818 - Ac3H11_375 0.20 +0.6 50,818 - Ac3H11_375 0.20 -0.3 50,818 - Ac3H11_375 0.20 +0.1 50,907 + Ac3H11_375 0.28 -0.1 50,907 + Ac3H11_375 0.28 +0.1 50,907 + Ac3H11_375 0.28 -0.1 50,907 + Ac3H11_375 0.28 -0.2 50,908 - Ac3H11_375 0.28 -0.7 50,908 - Ac3H11_375 0.28 -0.1 50,908 - Ac3H11_375 0.28 +0.4 51,034 - Ac3H11_375 0.39 -0.1 51,057 + Ac3H11_375 0.41 +0.7 51,057 + Ac3H11_375 0.41 -0.7 51,057 + Ac3H11_375 0.41 -0.1 51,057 + Ac3H11_375 0.41 +0.6 51,057 + Ac3H11_375 0.41 +1.8 51,057 + Ac3H11_375 0.41 -0.0 51,057 + Ac3H11_375 0.41 +1.1 51,057 + Ac3H11_375 0.41 +0.4 51,058 - Ac3H11_375 0.41 +0.5 51,058 - Ac3H11_375 0.41 +0.4 51,058 - Ac3H11_375 0.41 +0.2 51,058 - Ac3H11_375 0.41 -0.8 51,058 - Ac3H11_375 0.41 -0.6 51,253 - Ac3H11_375 0.59 +0.3 51,739 + -0.3 51,739 + -0.3 51,790 + -2.0 51,791 - -0.4 51,796 + +0.1 51,834 + +1.7 51,834 + -2.1 51,834 + -0.6 51,889 - -0.4 51,889 - -0.8 52,191 + Ac3H11_376 0.70 -3.3
Or see this region's nucleotide sequence