Experiment: Cephalothin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1481 and Ac3H11_1482 are separated by 138 nucleotides Ac3H11_1482 and Ac3H11_1483 are separated by 10 nucleotides Ac3H11_1483 and Ac3H11_1484 are separated by 70 nucleotides
Ac3H11_1481 - putative exported protein, at 60,927 to 61,907
_1481
Ac3H11_1482 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 62,046 to 62,870
_1482
Ac3H11_1483 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 62,881 to 63,630
_1483
Ac3H11_1484 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 63,701 to 64,156
_1484
Position (kb)
62
63 Strain fitness (log2 ratio)
-2
-1
0
1 at 61.064 kb on + strand, within Ac3H11_1481 at 61.064 kb on + strand, within Ac3H11_1481 at 61.065 kb on - strand, within Ac3H11_1481 at 61.065 kb on - strand, within Ac3H11_1481 at 61.435 kb on + strand, within Ac3H11_1481 at 61.435 kb on + strand, within Ac3H11_1481 at 61.435 kb on + strand, within Ac3H11_1481 at 61.435 kb on + strand, within Ac3H11_1481 at 61.435 kb on + strand, within Ac3H11_1481 at 61.435 kb on + strand, within Ac3H11_1481 at 61.435 kb on + strand, within Ac3H11_1481 at 61.436 kb on - strand, within Ac3H11_1481 at 61.436 kb on - strand, within Ac3H11_1481 at 61.436 kb on - strand, within Ac3H11_1481 at 61.436 kb on - strand, within Ac3H11_1481 at 61.573 kb on + strand, within Ac3H11_1481 at 61.574 kb on - strand, within Ac3H11_1481 at 61.574 kb on - strand, within Ac3H11_1481 at 61.589 kb on - strand, within Ac3H11_1481 at 61.666 kb on + strand, within Ac3H11_1481 at 61.666 kb on + strand, within Ac3H11_1481 at 61.666 kb on + strand, within Ac3H11_1481 at 62.028 kb on + strand at 62.028 kb on + strand at 62.028 kb on + strand at 62.029 kb on - strand at 62.029 kb on - strand at 62.029 kb on - strand at 62.029 kb on - strand at 62.029 kb on - strand at 62.029 kb on - strand at 62.029 kb on - strand at 62.029 kb on - strand at 62.050 kb on + strand at 62.130 kb on - strand, within Ac3H11_1482 at 62.194 kb on + strand, within Ac3H11_1482 at 62.194 kb on + strand, within Ac3H11_1482 at 62.459 kb on - strand, within Ac3H11_1482 at 62.459 kb on - strand, within Ac3H11_1482 at 62.530 kb on + strand, within Ac3H11_1482 at 62.531 kb on - strand, within Ac3H11_1482 at 62.531 kb on - strand, within Ac3H11_1482 at 62.614 kb on + strand, within Ac3H11_1482 at 62.614 kb on + strand, within Ac3H11_1482 at 62.614 kb on + strand, within Ac3H11_1482 at 62.918 kb on + strand at 63.287 kb on + strand, within Ac3H11_1483 at 63.287 kb on + strand, within Ac3H11_1483 at 63.288 kb on - strand, within Ac3H11_1483 at 63.288 kb on - strand, within Ac3H11_1483 at 63.402 kb on + strand, within Ac3H11_1483 at 63.402 kb on + strand, within Ac3H11_1483 at 63.402 kb on + strand, within Ac3H11_1483 at 63.403 kb on - strand, within Ac3H11_1483 at 63.403 kb on - strand, within Ac3H11_1483 at 63.446 kb on + strand, within Ac3H11_1483 at 63.447 kb on - strand, within Ac3H11_1483 at 63.630 kb on + strand at 63.630 kb on + strand at 63.630 kb on + strand at 63.630 kb on + strand at 63.630 kb on + strand at 63.630 kb on + strand at 63.630 kb on + strand at 63.631 kb on - strand at 63.631 kb on - strand at 63.631 kb on - strand at 63.631 kb on - strand at 63.631 kb on - strand at 63.631 kb on - strand at 63.631 kb on - strand at 63.631 kb on - strand at 63.692 kb on + strand at 63.692 kb on + strand at 63.693 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.005 mg/ml remove 61,064 + Ac3H11_1481 0.14 -0.2 61,064 + Ac3H11_1481 0.14 +0.1 61,065 - Ac3H11_1481 0.14 +0.7 61,065 - Ac3H11_1481 0.14 +0.1 61,435 + Ac3H11_1481 0.52 +0.4 61,435 + Ac3H11_1481 0.52 +0.4 61,435 + Ac3H11_1481 0.52 +1.0 61,435 + Ac3H11_1481 0.52 +0.6 61,435 + Ac3H11_1481 0.52 -0.4 61,435 + Ac3H11_1481 0.52 +0.6 61,435 + Ac3H11_1481 0.52 -0.2 61,436 - Ac3H11_1481 0.52 +0.3 61,436 - Ac3H11_1481 0.52 -0.1 61,436 - Ac3H11_1481 0.52 +1.1 61,436 - Ac3H11_1481 0.52 -0.3 61,573 + Ac3H11_1481 0.66 -1.5 61,574 - Ac3H11_1481 0.66 -0.0 61,574 - Ac3H11_1481 0.66 +0.2 61,589 - Ac3H11_1481 0.67 +1.7 61,666 + Ac3H11_1481 0.75 +0.4 61,666 + Ac3H11_1481 0.75 +1.0 61,666 + Ac3H11_1481 0.75 -0.3 62,028 + +1.6 62,028 + -0.2 62,028 + -0.5 62,029 - +0.4 62,029 - +0.3 62,029 - -0.6 62,029 - +0.1 62,029 - -0.2 62,029 - +0.5 62,029 - -0.4 62,029 - +0.3 62,050 + -1.0 62,130 - Ac3H11_1482 0.10 +1.2 62,194 + Ac3H11_1482 0.18 -0.0 62,194 + Ac3H11_1482 0.18 -0.4 62,459 - Ac3H11_1482 0.50 -1.9 62,459 - Ac3H11_1482 0.50 +0.1 62,530 + Ac3H11_1482 0.59 +1.1 62,531 - Ac3H11_1482 0.59 +1.3 62,531 - Ac3H11_1482 0.59 +0.1 62,614 + Ac3H11_1482 0.69 +0.4 62,614 + Ac3H11_1482 0.69 -0.4 62,614 + Ac3H11_1482 0.69 +1.0 62,918 + +0.1 63,287 + Ac3H11_1483 0.54 +0.7 63,287 + Ac3H11_1483 0.54 +0.5 63,288 - Ac3H11_1483 0.54 +0.1 63,288 - Ac3H11_1483 0.54 -0.5 63,402 + Ac3H11_1483 0.69 +0.5 63,402 + Ac3H11_1483 0.69 -0.0 63,402 + Ac3H11_1483 0.69 +1.5 63,403 - Ac3H11_1483 0.70 +1.6 63,403 - Ac3H11_1483 0.70 +0.1 63,446 + Ac3H11_1483 0.75 +0.7 63,447 - Ac3H11_1483 0.75 +0.2 63,630 + +0.5 63,630 + +0.4 63,630 + -0.3 63,630 + +0.1 63,630 + +0.6 63,630 + +0.7 63,630 + -0.4 63,631 - +0.3 63,631 - +0.7 63,631 - +1.5 63,631 - -0.1 63,631 - -0.0 63,631 - +0.1 63,631 - -0.3 63,631 - +0.3 63,692 + -1.7 63,692 + +0.0 63,693 - -0.3
Or see this region's nucleotide sequence