Experiment: L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS23655 and HSERO_RS23660 overlap by 4 nucleotides HSERO_RS23660 and HSERO_RS23665 are separated by 182 nucleotides HSERO_RS23665 and HSERO_RS23670 are separated by 258 nucleotides HSERO_RS23670 and HSERO_RS23675 are separated by 2 nucleotides
HSERO_RS23655: HSERO_RS23655 - phosphonate ABC transporter permease, at 5,422,466 to 5,423,269
_RS23655
HSERO_RS23660: HSERO_RS23660 - phosphonate ABC transporter ATP-binding protein, at 5,423,266 to 5,424,036
_RS23660
HSERO_RS23665: HSERO_RS23665 - phosphonate ABC transporter substrate-binding protein, at 5,424,219 to 5,425,208
_RS23665
HSERO_RS23670: HSERO_RS23670 - chemotaxis protein CheY, at 5,425,467 to 5,426,153
_RS23670
HSERO_RS23675: HSERO_RS23675 - histidine kinase, at 5,426,156 to 5,427,559
_RS23675
Position (kb)
5424
5425
5426 Strain fitness (log2 ratio)
-2
-1
0
1 at 5423.296 kb on - strand at 5423.386 kb on - strand, within HSERO_RS23660 at 5423.613 kb on + strand, within HSERO_RS23660 at 5423.629 kb on - strand, within HSERO_RS23660 at 5423.639 kb on - strand, within HSERO_RS23660 at 5423.703 kb on + strand, within HSERO_RS23660 at 5423.970 kb on + strand at 5424.034 kb on - strand at 5424.266 kb on - strand at 5424.289 kb on - strand at 5424.578 kb on + strand, within HSERO_RS23665 at 5424.585 kb on - strand, within HSERO_RS23665 at 5424.586 kb on - strand, within HSERO_RS23665 at 5424.586 kb on - strand, within HSERO_RS23665 at 5424.747 kb on - strand, within HSERO_RS23665 at 5424.763 kb on - strand, within HSERO_RS23665 at 5424.775 kb on - strand, within HSERO_RS23665 at 5425.090 kb on - strand, within HSERO_RS23665 at 5425.199 kb on - strand at 5425.258 kb on + strand at 5425.573 kb on - strand, within HSERO_RS23670 at 5425.654 kb on - strand, within HSERO_RS23670 at 5425.927 kb on + strand, within HSERO_RS23670 at 5426.055 kb on - strand, within HSERO_RS23670 at 5426.186 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine remove 5,423,296 - -0.7 5,423,386 - HSERO_RS23660 0.16 +1.0 5,423,613 + HSERO_RS23660 0.45 -0.1 5,423,629 - HSERO_RS23660 0.47 -0.0 5,423,639 - HSERO_RS23660 0.48 -0.1 5,423,703 + HSERO_RS23660 0.57 -0.0 5,423,970 + +0.3 5,424,034 - +0.4 5,424,266 - -0.1 5,424,289 - +0.2 5,424,578 + HSERO_RS23665 0.36 +0.3 5,424,585 - HSERO_RS23665 0.37 -2.5 5,424,586 - HSERO_RS23665 0.37 -0.0 5,424,586 - HSERO_RS23665 0.37 +0.5 5,424,747 - HSERO_RS23665 0.53 +0.4 5,424,763 - HSERO_RS23665 0.55 +0.3 5,424,775 - HSERO_RS23665 0.56 +1.3 5,425,090 - HSERO_RS23665 0.88 +0.2 5,425,199 - -0.6 5,425,258 + -0.0 5,425,573 - HSERO_RS23670 0.15 +0.2 5,425,654 - HSERO_RS23670 0.27 -0.0 5,425,927 + HSERO_RS23670 0.67 +0.3 5,426,055 - HSERO_RS23670 0.86 -0.4 5,426,186 + +0.5
Or see this region's nucleotide sequence