Experiment: L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS11210 and HSERO_RS11215 are separated by 48 nucleotides HSERO_RS11215 and HSERO_RS11220 are separated by 46 nucleotides HSERO_RS11220 and HSERO_RS11225 are separated by 128 nucleotides
HSERO_RS11210: HSERO_RS11210 - septation protein A, at 2,533,331 to 2,533,951
_RS11210
HSERO_RS11215: HSERO_RS11215 - methionine sulfoxide reductase B, at 2,534,000 to 2,534,395
_RS11215
HSERO_RS11220: HSERO_RS11220 - hypothetical protein, at 2,534,442 to 2,535,929
_RS11220
HSERO_RS11225: HSERO_RS11225 - branched-chain amino acid ABC transporter permease, at 2,536,058 to 2,536,993
_RS11225
Position (kb)
2534
2535
2536 Strain fitness (log2 ratio)
-1
0
1 at 2533.985 kb on + strand at 2534.006 kb on - strand at 2534.175 kb on - strand, within HSERO_RS11215 at 2534.255 kb on + strand, within HSERO_RS11215 at 2534.262 kb on + strand, within HSERO_RS11215 at 2534.368 kb on - strand at 2534.514 kb on - strand at 2534.524 kb on + strand at 2534.590 kb on + strand at 2534.697 kb on + strand, within HSERO_RS11220 at 2534.715 kb on + strand, within HSERO_RS11220 at 2534.888 kb on + strand, within HSERO_RS11220 at 2534.907 kb on - strand, within HSERO_RS11220 at 2534.911 kb on + strand, within HSERO_RS11220 at 2535.039 kb on + strand, within HSERO_RS11220 at 2535.092 kb on + strand, within HSERO_RS11220 at 2535.100 kb on - strand, within HSERO_RS11220 at 2535.298 kb on + strand, within HSERO_RS11220 at 2535.579 kb on - strand, within HSERO_RS11220 at 2535.771 kb on + strand, within HSERO_RS11220 at 2535.815 kb on + strand at 2535.952 kb on - strand at 2536.085 kb on - strand at 2536.267 kb on + strand, within HSERO_RS11225 at 2536.365 kb on - strand, within HSERO_RS11225 at 2536.365 kb on - strand, within HSERO_RS11225 at 2536.452 kb on - strand, within HSERO_RS11225 at 2536.490 kb on - strand, within HSERO_RS11225 at 2536.576 kb on - strand, within HSERO_RS11225 at 2536.576 kb on - strand, within HSERO_RS11225 at 2536.763 kb on - strand, within HSERO_RS11225 at 2536.845 kb on + strand, within HSERO_RS11225 at 2536.903 kb on + strand at 2536.919 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine remove 2,533,985 + +0.8 2,534,006 - -0.3 2,534,175 - HSERO_RS11215 0.44 -0.2 2,534,255 + HSERO_RS11215 0.64 -0.5 2,534,262 + HSERO_RS11215 0.66 +0.3 2,534,368 - +1.0 2,534,514 - -0.1 2,534,524 + -0.0 2,534,590 + +0.3 2,534,697 + HSERO_RS11220 0.17 -0.1 2,534,715 + HSERO_RS11220 0.18 +0.7 2,534,888 + HSERO_RS11220 0.30 +0.2 2,534,907 - HSERO_RS11220 0.31 -0.9 2,534,911 + HSERO_RS11220 0.32 +0.2 2,535,039 + HSERO_RS11220 0.40 -0.7 2,535,092 + HSERO_RS11220 0.44 +0.1 2,535,100 - HSERO_RS11220 0.44 +0.6 2,535,298 + HSERO_RS11220 0.58 +0.9 2,535,579 - HSERO_RS11220 0.76 +0.9 2,535,771 + HSERO_RS11220 0.89 +0.8 2,535,815 + -0.1 2,535,952 - +0.2 2,536,085 - +0.0 2,536,267 + HSERO_RS11225 0.22 -0.5 2,536,365 - HSERO_RS11225 0.33 +0.7 2,536,365 - HSERO_RS11225 0.33 -0.0 2,536,452 - HSERO_RS11225 0.42 +0.7 2,536,490 - HSERO_RS11225 0.46 -0.3 2,536,576 - HSERO_RS11225 0.55 -0.1 2,536,576 - HSERO_RS11225 0.55 +0.6 2,536,763 - HSERO_RS11225 0.75 +0.2 2,536,845 + HSERO_RS11225 0.84 -1.1 2,536,903 + -0.8 2,536,919 + -0.1
Or see this region's nucleotide sequence