Experiment: L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS11170 and HSERO_RS11175 are separated by 16 nucleotides HSERO_RS11175 and HSERO_RS11180 overlap by 47 nucleotides HSERO_RS11180 and HSERO_RS11185 are separated by 150 nucleotides
HSERO_RS11170: HSERO_RS11170 - ABC transporter ATP-binding protein, at 2,521,270 to 2,521,974
_RS11170
HSERO_RS11175: HSERO_RS11175 - multidrug DMT transporter permease, at 2,521,991 to 2,522,935
_RS11175
HSERO_RS11180: HSERO_RS11180 - sugar kinase, at 2,522,889 to 2,524,457
_RS11180
HSERO_RS11185: HSERO_RS11185 - amino acid dehydrogenase, at 2,524,608 to 2,525,879
_RS11185
Position (kb)
2522
2523
2524
2525 Strain fitness (log2 ratio)
-1
0
1 at 2521.894 kb on - strand, within HSERO_RS11170 at 2522.304 kb on + strand, within HSERO_RS11175 at 2522.338 kb on - strand, within HSERO_RS11175 at 2522.351 kb on - strand, within HSERO_RS11175 at 2522.429 kb on - strand, within HSERO_RS11175 at 2522.595 kb on + strand, within HSERO_RS11175 at 2522.772 kb on - strand, within HSERO_RS11175 at 2522.788 kb on - strand, within HSERO_RS11175 at 2522.815 kb on + strand, within HSERO_RS11175 at 2522.861 kb on - strand at 2522.925 kb on + strand at 2522.930 kb on + strand at 2522.933 kb on - strand at 2522.996 kb on + strand at 2523.139 kb on + strand, within HSERO_RS11180 at 2523.149 kb on + strand, within HSERO_RS11180 at 2523.232 kb on + strand, within HSERO_RS11180 at 2523.508 kb on - strand, within HSERO_RS11180 at 2523.999 kb on + strand, within HSERO_RS11180 at 2524.034 kb on + strand, within HSERO_RS11180 at 2524.483 kb on - strand at 2524.566 kb on - strand at 2524.611 kb on + strand at 2524.619 kb on - strand at 2524.815 kb on - strand, within HSERO_RS11185 at 2525.256 kb on - strand, within HSERO_RS11185
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine remove 2,521,894 - HSERO_RS11170 0.89 -0.3 2,522,304 + HSERO_RS11175 0.33 +0.2 2,522,338 - HSERO_RS11175 0.37 +0.2 2,522,351 - HSERO_RS11175 0.38 +0.0 2,522,429 - HSERO_RS11175 0.46 -0.3 2,522,595 + HSERO_RS11175 0.64 +0.4 2,522,772 - HSERO_RS11175 0.83 -0.4 2,522,788 - HSERO_RS11175 0.84 -0.9 2,522,815 + HSERO_RS11175 0.87 +1.5 2,522,861 - +0.3 2,522,925 + -0.0 2,522,930 + -0.8 2,522,933 - -0.4 2,522,996 + +1.0 2,523,139 + HSERO_RS11180 0.16 +0.5 2,523,149 + HSERO_RS11180 0.17 +0.8 2,523,232 + HSERO_RS11180 0.22 -0.0 2,523,508 - HSERO_RS11180 0.39 +0.8 2,523,999 + HSERO_RS11180 0.71 -0.2 2,524,034 + HSERO_RS11180 0.73 -0.7 2,524,483 - -0.2 2,524,566 - -0.4 2,524,611 + +0.5 2,524,619 - -0.0 2,524,815 - HSERO_RS11185 0.16 +0.1 2,525,256 - HSERO_RS11185 0.51 -0.5
Or see this region's nucleotide sequence