Experiment: L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS10760 and HSERO_RS10765 are separated by 219 nucleotides HSERO_RS10765 and HSERO_RS10770 are separated by 122 nucleotides HSERO_RS10770 and HSERO_RS10775 are separated by 86 nucleotides
HSERO_RS10760: HSERO_RS10760 - chemotaxis protein CheY, at 2,419,416 to 2,420,177
_RS10760
HSERO_RS10765: HSERO_RS10765 - hypothetical protein, at 2,420,397 to 2,420,678
_RS10765
HSERO_RS10770: HSERO_RS10770 - amino acid ABC transporter permease, at 2,420,801 to 2,421,466
_RS10770
HSERO_RS10775: HSERO_RS10775 - cysteine ABC transporter substrate-binding protein, at 2,421,553 to 2,422,356
_RS10775
Position (kb)
2420
2421 Strain fitness (log2 ratio)
-1
0
1
2 at 2419.452 kb on - strand at 2419.516 kb on + strand, within HSERO_RS10760 at 2420.031 kb on - strand, within HSERO_RS10760 at 2420.212 kb on + strand at 2420.240 kb on + strand at 2420.279 kb on - strand at 2420.544 kb on - strand, within HSERO_RS10765 at 2420.557 kb on - strand, within HSERO_RS10765 at 2420.601 kb on - strand, within HSERO_RS10765 at 2420.925 kb on + strand, within HSERO_RS10770 at 2420.985 kb on - strand, within HSERO_RS10770 at 2421.157 kb on + strand, within HSERO_RS10770 at 2421.373 kb on + strand, within HSERO_RS10770 at 2421.399 kb on + strand, within HSERO_RS10770 at 2421.419 kb on - strand at 2421.579 kb on + strand at 2421.644 kb on + strand, within HSERO_RS10775 at 2421.652 kb on - strand, within HSERO_RS10775
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine remove 2,419,452 - +0.2 2,419,516 + HSERO_RS10760 0.13 +1.4 2,420,031 - HSERO_RS10760 0.81 -0.6 2,420,212 + +0.3 2,420,240 + -0.7 2,420,279 - +0.6 2,420,544 - HSERO_RS10765 0.52 +0.2 2,420,557 - HSERO_RS10765 0.57 +1.1 2,420,601 - HSERO_RS10765 0.72 +2.7 2,420,925 + HSERO_RS10770 0.19 -0.2 2,420,985 - HSERO_RS10770 0.28 +0.3 2,421,157 + HSERO_RS10770 0.53 +0.4 2,421,373 + HSERO_RS10770 0.86 +0.6 2,421,399 + HSERO_RS10770 0.90 +0.1 2,421,419 - -0.5 2,421,579 + -0.5 2,421,644 + HSERO_RS10775 0.11 -0.1 2,421,652 - HSERO_RS10775 0.12 -0.5
Or see this region's nucleotide sequence