Experiment: L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS10310 and HSERO_RS10315 are separated by 250 nucleotides HSERO_RS10315 and HSERO_RS10320 are separated by 120 nucleotides HSERO_RS10320 and HSERO_RS10325 are separated by 22 nucleotides
HSERO_RS10310: HSERO_RS10310 - flagellar M-ring protein FliF, at 2,322,901 to 2,324,682
_RS10310
HSERO_RS10315: HSERO_RS10315 - flagellar hook-basal body protein FliE, at 2,324,933 to 2,325,289
_RS10315
HSERO_RS10320: HSERO_RS10320 - flagellar brake protein YcgR, at 2,325,410 to 2,326,165
_RS10320
HSERO_RS10325: HSERO_RS10325 - flagellar biosynthesis protein, at 2,326,188 to 2,326,532
_RS10325
Position (kb)
2324
2325
2326 Strain fitness (log2 ratio)
-1
0
1 at 2324.305 kb on - strand, within HSERO_RS10310 at 2324.347 kb on + strand, within HSERO_RS10310 at 2324.554 kb on - strand at 2324.602 kb on - strand at 2324.616 kb on + strand at 2324.735 kb on + strand at 2324.735 kb on + strand at 2324.764 kb on + strand at 2324.805 kb on - strand at 2324.994 kb on - strand at 2325.096 kb on + strand, within HSERO_RS10315 at 2325.106 kb on + strand, within HSERO_RS10315 at 2325.106 kb on + strand, within HSERO_RS10315 at 2325.249 kb on - strand, within HSERO_RS10315 at 2325.258 kb on - strand at 2325.258 kb on - strand at 2325.528 kb on + strand, within HSERO_RS10320 at 2325.849 kb on - strand, within HSERO_RS10320 at 2326.126 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine remove 2,324,305 - HSERO_RS10310 0.79 -0.7 2,324,347 + HSERO_RS10310 0.81 +1.3 2,324,554 - +1.0 2,324,602 - +0.4 2,324,616 + +1.2 2,324,735 + +0.9 2,324,735 + +1.1 2,324,764 + +1.1 2,324,805 - +1.1 2,324,994 - -0.1 2,325,096 + HSERO_RS10315 0.46 -0.4 2,325,106 + HSERO_RS10315 0.48 +0.5 2,325,106 + HSERO_RS10315 0.48 +0.8 2,325,249 - HSERO_RS10315 0.89 +0.5 2,325,258 - +0.7 2,325,258 - +0.7 2,325,528 + HSERO_RS10320 0.16 +0.3 2,325,849 - HSERO_RS10320 0.58 -1.5 2,326,126 + -0.1
Or see this region's nucleotide sequence