Experiment: L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS04220 and HSERO_RS04225 are separated by 12 nucleotides HSERO_RS04225 and HSERO_RS04230 are separated by 15 nucleotides HSERO_RS04230 and HSERO_RS04235 are separated by 53 nucleotides
HSERO_RS04220: HSERO_RS04220 - epoxyqueuosine reductase, at 926,945 to 928,090
_RS04220
HSERO_RS04225: HSERO_RS04225 - permease, at 928,103 to 928,993
_RS04225
HSERO_RS04230: HSERO_RS04230 - LuxR family transcriptional regulator, at 929,009 to 929,599
_RS04230
HSERO_RS04235: HSERO_RS04235 - C4-dicarboxylate ABC transporter, at 929,653 to 931,677
_RS04235
Position (kb) 929
930 Strain fitness (log2 ratio) -2
-1
0
1 at 928.032 kb on + strand at 928.119 kb on + strand at 928.191 kb on - strand at 928.444 kb on + strand, within HSERO_RS04225 at 928.489 kb on + strand, within HSERO_RS04225 at 928.666 kb on - strand, within HSERO_RS04225 at 928.720 kb on - strand, within HSERO_RS04225 at 928.733 kb on - strand, within HSERO_RS04225 at 928.801 kb on - strand, within HSERO_RS04225 at 928.835 kb on - strand, within HSERO_RS04225 at 928.918 kb on + strand at 928.937 kb on - strand at 929.192 kb on + strand, within HSERO_RS04230 at 929.207 kb on - strand, within HSERO_RS04230 at 929.330 kb on - strand, within HSERO_RS04230 at 929.596 kb on + strand at 929.725 kb on - strand at 930.008 kb on - strand at 930.090 kb on + strand, within HSERO_RS04235 at 930.188 kb on + strand, within HSERO_RS04235 at 930.278 kb on - strand, within HSERO_RS04235 at 930.281 kb on - strand, within HSERO_RS04235 at 930.510 kb on - strand at 930.538 kb on - strand, within HSERO_RS04235 at 930.564 kb on - strand, within HSERO_RS04235 at 930.574 kb on - strand, within HSERO_RS04235
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine remove 928,032 + +0.1 928,119 + -0.3 928,191 - -0.3 928,444 + HSERO_RS04225 0.38 +0.3 928,489 + HSERO_RS04225 0.43 -0.2 928,666 - HSERO_RS04225 0.63 -0.1 928,720 - HSERO_RS04225 0.69 -2.0 928,733 - HSERO_RS04225 0.71 -0.5 928,801 - HSERO_RS04225 0.78 +1.6 928,835 - HSERO_RS04225 0.82 +0.3 928,918 + -0.3 928,937 - +0.4 929,192 + HSERO_RS04230 0.31 +1.0 929,207 - HSERO_RS04230 0.34 -1.9 929,330 - HSERO_RS04230 0.54 -0.1 929,596 + -0.0 929,725 - +0.1 930,008 - -1.9 930,090 + HSERO_RS04235 0.22 +0.2 930,188 + HSERO_RS04235 0.26 +1.0 930,278 - HSERO_RS04235 0.31 -0.1 930,281 - HSERO_RS04235 0.31 -2.3 930,510 - -0.1 930,538 - HSERO_RS04235 0.44 -0.8 930,564 - HSERO_RS04235 0.45 -0.8 930,574 - HSERO_RS04235 0.45 +0.3
Or see this region's nucleotide sequence