Experiment: L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02395 and HSERO_RS02400 are separated by 126 nucleotides HSERO_RS02400 and HSERO_RS02405 are separated by 619 nucleotides HSERO_RS02405 and HSERO_RS02410 are separated by 2 nucleotides
HSERO_RS02395: HSERO_RS02395 - 23S ribosomal RNA, at 521,732 to 524,621
_RS02395
HSERO_RS02400: HSERO_RS02400 - 5S ribosomal RNA, at 524,748 to 524,860
_RS02400
HSERO_RS02405: HSERO_RS02405 - multidrug transporter, at 525,480 to 526,931
_RS02405
HSERO_RS02410: HSERO_RS02410 - acriflavine resistance protein B, at 526,934 to 530,080
_RS02410
Position (kb) 525
526
527 Strain fitness (log2 ratio) -1
0
1 at 525.195 kb on + strand at 525.336 kb on + strand at 525.393 kb on - strand at 525.393 kb on - strand at 525.540 kb on - strand at 525.587 kb on - strand at 525.831 kb on + strand, within HSERO_RS02405 at 525.918 kb on - strand, within HSERO_RS02405 at 526.263 kb on + strand, within HSERO_RS02405 at 526.323 kb on + strand, within HSERO_RS02405 at 526.344 kb on - strand, within HSERO_RS02405 at 526.359 kb on - strand, within HSERO_RS02405 at 526.359 kb on - strand, within HSERO_RS02405 at 526.872 kb on + strand at 526.984 kb on + strand at 527.231 kb on - strand at 527.324 kb on - strand, within HSERO_RS02410 at 527.351 kb on + strand, within HSERO_RS02410 at 527.766 kb on + strand, within HSERO_RS02410 at 527.897 kb on - strand, within HSERO_RS02410
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine remove 525,195 + +0.8 525,336 + +1.0 525,393 - -1.3 525,393 - -0.8 525,540 - -0.4 525,587 - +0.4 525,831 + HSERO_RS02405 0.24 +0.4 525,918 - HSERO_RS02405 0.30 +0.5 526,263 + HSERO_RS02405 0.54 +0.7 526,323 + HSERO_RS02405 0.58 +1.0 526,344 - HSERO_RS02405 0.60 -0.2 526,359 - HSERO_RS02405 0.61 -0.0 526,359 - HSERO_RS02405 0.61 -0.1 526,872 + +0.2 526,984 + +0.4 527,231 - +0.6 527,324 - HSERO_RS02410 0.12 +0.2 527,351 + HSERO_RS02410 0.13 +0.4 527,766 + HSERO_RS02410 0.26 +0.4 527,897 - HSERO_RS02410 0.31 -0.0
Or see this region's nucleotide sequence