Experiment: L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01980 and HSERO_RS01985 are separated by 67 nucleotides HSERO_RS01985 and HSERO_RS01990 are separated by 88 nucleotides HSERO_RS01990 and HSERO_RS01995 are separated by 26 nucleotides
HSERO_RS01980: HSERO_RS01980 - ribonuclease II, at 433,147 to 435,201
_RS01980
HSERO_RS01985: HSERO_RS01985 - energy transducer TonB, at 435,269 to 436,150
_RS01985
HSERO_RS01990: HSERO_RS01990 - shikimate 5-dehydrogenase, at 436,239 to 437,054
_RS01990
HSERO_RS01995: HSERO_RS01995 - peptidoglycan transglycosylase, at 437,081 to 437,788
_RS01995
Position (kb) 435
436
437 Strain fitness (log2 ratio) -2
-1
0
1 at 435.193 kb on + strand at 435.225 kb on - strand at 435.232 kb on - strand at 435.267 kb on + strand at 435.308 kb on - strand at 435.308 kb on - strand at 435.313 kb on - strand at 435.333 kb on + strand at 435.341 kb on - strand at 435.368 kb on - strand, within HSERO_RS01985 at 435.694 kb on + strand, within HSERO_RS01985 at 435.795 kb on + strand, within HSERO_RS01985 at 435.830 kb on + strand, within HSERO_RS01985 at 436.226 kb on + strand at 436.449 kb on - strand, within HSERO_RS01990 at 436.484 kb on - strand, within HSERO_RS01990 at 436.551 kb on - strand at 436.570 kb on - strand, within HSERO_RS01990 at 436.853 kb on - strand, within HSERO_RS01990 at 436.875 kb on - strand, within HSERO_RS01990 at 437.112 kb on - strand at 437.117 kb on + strand at 437.117 kb on + strand at 437.132 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine remove 435,193 + -0.1 435,225 - -0.2 435,232 - +0.1 435,267 + -0.2 435,308 - -0.0 435,308 - -0.2 435,313 - +0.2 435,333 + +0.8 435,341 - +0.9 435,368 - HSERO_RS01985 0.11 +0.7 435,694 + HSERO_RS01985 0.48 +0.0 435,795 + HSERO_RS01985 0.60 +0.6 435,830 + HSERO_RS01985 0.64 +0.6 436,226 + -0.0 436,449 - HSERO_RS01990 0.26 -1.1 436,484 - HSERO_RS01990 0.30 -1.6 436,551 - -1.6 436,570 - HSERO_RS01990 0.41 -1.6 436,853 - HSERO_RS01990 0.75 -2.1 436,875 - HSERO_RS01990 0.78 -1.4 437,112 - -0.4 437,117 + -0.2 437,117 + +1.0 437,132 - -1.9
Or see this region's nucleotide sequence