Experiment: L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01935 and HSERO_RS01940 are separated by 96 nucleotides HSERO_RS01940 and HSERO_RS01945 overlap by 1 nucleotides HSERO_RS01945 and HSERO_RS01950 are separated by 40 nucleotides
HSERO_RS01935: HSERO_RS01935 - 3-dehydroquinate dehydratase, at 425,391 to 425,828
_RS01935
HSERO_RS01940: HSERO_RS01940 - thioredoxin, at 425,925 to 426,446
_RS01940
HSERO_RS01945: HSERO_RS01945 - hypothetical protein, at 426,446 to 427,087
_RS01945
HSERO_RS01950: HSERO_RS01950 - transcription regulator protein, at 427,128 to 428,165
_RS01950
Position (kb)
426
427
428 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 425.456 kb on - strand, within HSERO_RS01935 at 425.573 kb on - strand, within HSERO_RS01935 at 425.779 kb on - strand, within HSERO_RS01935 at 426.490 kb on - strand at 426.647 kb on - strand, within HSERO_RS01945 at 426.756 kb on - strand, within HSERO_RS01945 at 426.977 kb on - strand, within HSERO_RS01945 at 426.994 kb on - strand, within HSERO_RS01945 at 427.080 kb on + strand at 427.080 kb on + strand at 427.085 kb on + strand at 427.093 kb on - strand at 427.116 kb on + strand at 427.158 kb on + strand at 427.161 kb on - strand at 427.169 kb on + strand at 427.201 kb on - strand at 427.222 kb on - strand at 427.355 kb on + strand, within HSERO_RS01950 at 427.407 kb on - strand, within HSERO_RS01950 at 427.561 kb on + strand, within HSERO_RS01950 at 427.607 kb on - strand, within HSERO_RS01950 at 427.721 kb on + strand, within HSERO_RS01950 at 427.772 kb on + strand, within HSERO_RS01950 at 427.795 kb on + strand, within HSERO_RS01950 at 427.847 kb on - strand, within HSERO_RS01950 at 427.906 kb on + strand, within HSERO_RS01950 at 427.907 kb on - strand, within HSERO_RS01950 at 427.914 kb on - strand, within HSERO_RS01950 at 427.920 kb on + strand, within HSERO_RS01950 at 427.931 kb on + strand, within HSERO_RS01950 at 427.939 kb on - strand, within HSERO_RS01950 at 427.958 kb on + strand, within HSERO_RS01950 at 427.984 kb on - strand, within HSERO_RS01950 at 428.000 kb on - strand, within HSERO_RS01950 at 428.072 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine remove 425,456 - HSERO_RS01935 0.15 -5.2 425,573 - HSERO_RS01935 0.42 -4.2 425,779 - HSERO_RS01935 0.89 -0.6 426,490 - -0.3 426,647 - HSERO_RS01945 0.31 +0.6 426,756 - HSERO_RS01945 0.48 +0.1 426,977 - HSERO_RS01945 0.83 +1.0 426,994 - HSERO_RS01945 0.85 +0.4 427,080 + +0.1 427,080 + -0.1 427,085 + -1.5 427,093 - +0.0 427,116 + +0.2 427,158 + -0.3 427,161 - +0.8 427,169 + +0.4 427,201 - +0.4 427,222 - +1.3 427,355 + HSERO_RS01950 0.22 +0.1 427,407 - HSERO_RS01950 0.27 -1.1 427,561 + HSERO_RS01950 0.42 +0.8 427,607 - HSERO_RS01950 0.46 -0.1 427,721 + HSERO_RS01950 0.57 -0.7 427,772 + HSERO_RS01950 0.62 +1.0 427,795 + HSERO_RS01950 0.64 -0.5 427,847 - HSERO_RS01950 0.69 +0.5 427,906 + HSERO_RS01950 0.75 +0.5 427,907 - HSERO_RS01950 0.75 +1.0 427,914 - HSERO_RS01950 0.76 -1.4 427,920 + HSERO_RS01950 0.76 +0.7 427,931 + HSERO_RS01950 0.77 +0.2 427,939 - HSERO_RS01950 0.78 +0.4 427,958 + HSERO_RS01950 0.80 +0.1 427,984 - HSERO_RS01950 0.82 -0.2 428,000 - HSERO_RS01950 0.84 -0.0 428,072 + +0.5
Or see this region's nucleotide sequence