Experiment: L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01910 and HSERO_RS01915 are separated by 100 nucleotides HSERO_RS01915 and HSERO_RS01920 are separated by 3 nucleotides HSERO_RS01920 and HSERO_RS01925 are separated by 41 nucleotides
HSERO_RS01910: HSERO_RS01910 - sugar kinase, at 420,023 to 420,958
_RS01910
HSERO_RS01915: HSERO_RS01915 - membrane protein, at 421,059 to 422,348
_RS01915
HSERO_RS01920: HSERO_RS01920 - 50S ribosomal protein L11 methyltransferase, at 422,352 to 423,266
_RS01920
HSERO_RS01925: HSERO_RS01925 - acetyl-CoA carboxylase biotin carboxylase subunit, at 423,308 to 424,687
_RS01925
Position (kb)
421
422
423 Strain fitness (log2 ratio)
-2
-1
0
1 at 421.019 kb on + strand at 421.036 kb on - strand at 421.051 kb on + strand at 421.094 kb on - strand at 421.243 kb on + strand, within HSERO_RS01915 at 421.411 kb on + strand, within HSERO_RS01915 at 421.555 kb on - strand, within HSERO_RS01915 at 421.627 kb on - strand, within HSERO_RS01915 at 421.807 kb on - strand at 422.168 kb on - strand, within HSERO_RS01915 at 422.303 kb on + strand at 422.413 kb on - strand at 422.510 kb on + strand, within HSERO_RS01920 at 422.518 kb on - strand, within HSERO_RS01920 at 422.609 kb on + strand, within HSERO_RS01920 at 422.751 kb on + strand, within HSERO_RS01920 at 422.972 kb on - strand, within HSERO_RS01920 at 423.001 kb on + strand, within HSERO_RS01920 at 423.014 kb on - strand, within HSERO_RS01920
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine remove 421,019 + +0.4 421,036 - +0.1 421,051 + -0.6 421,094 - +0.4 421,243 + HSERO_RS01915 0.14 +1.0 421,411 + HSERO_RS01915 0.27 +0.1 421,555 - HSERO_RS01915 0.38 +0.8 421,627 - HSERO_RS01915 0.44 +0.3 421,807 - -0.0 422,168 - HSERO_RS01915 0.86 +0.3 422,303 + +0.6 422,413 - +0.2 422,510 + HSERO_RS01920 0.17 -0.4 422,518 - HSERO_RS01920 0.18 +0.3 422,609 + HSERO_RS01920 0.28 +0.1 422,751 + HSERO_RS01920 0.44 +0.4 422,972 - HSERO_RS01920 0.68 +1.0 423,001 + HSERO_RS01920 0.71 -0.1 423,014 - HSERO_RS01920 0.72 -1.9
Or see this region's nucleotide sequence