Experiment: L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS00795 and HSERO_RS00800 are separated by 12 nucleotides HSERO_RS00800 and HSERO_RS00805 are separated by 117 nucleotides HSERO_RS00805 and HSERO_RS00810 are separated by 52 nucleotides HSERO_RS00810 and HSERO_RS00815 overlap by 4 nucleotides HSERO_RS00815 and HSERO_RS00820 are separated by 150 nucleotides
HSERO_RS00795: HSERO_RS00795 - glycerol-3-phosphate dehydrogenase, at 166,667 to 167,671
_RS00795
HSERO_RS00800: HSERO_RS00800 - hypothetical protein, at 167,684 to 168,133
_RS00800
HSERO_RS00805: HSERO_RS00805 - preprotein translocase subunit SecB, at 168,251 to 168,745
_RS00805
HSERO_RS00810: HSERO_RS00810 - glutaredoxin, at 168,798 to 169,058
_RS00810
HSERO_RS00815: HSERO_RS00815 - sulfurtransferase, at 169,055 to 169,468
_RS00815
HSERO_RS00820: HSERO_RS00820 - phosphoglyceromutase, at 169,619 to 170,365
_RS00820
Position (kb) 168
169 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 167.256 kb on - strand, within HSERO_RS00795 at 167.347 kb on - strand, within HSERO_RS00795 at 167.592 kb on + strand at 167.827 kb on - strand, within HSERO_RS00800 at 167.869 kb on + strand, within HSERO_RS00800 at 167.901 kb on + strand, within HSERO_RS00800 at 167.909 kb on - strand, within HSERO_RS00800 at 167.925 kb on + strand, within HSERO_RS00800 at 167.988 kb on + strand, within HSERO_RS00800 at 167.996 kb on - strand, within HSERO_RS00800 at 168.083 kb on - strand, within HSERO_RS00800 at 168.242 kb on - strand at 168.291 kb on - strand at 168.330 kb on - strand, within HSERO_RS00805 at 168.435 kb on - strand, within HSERO_RS00805 at 168.751 kb on + strand at 168.776 kb on + strand at 168.776 kb on + strand at 168.787 kb on - strand at 168.888 kb on - strand, within HSERO_RS00810 at 169.212 kb on + strand, within HSERO_RS00815 at 169.410 kb on + strand, within HSERO_RS00815 at 169.423 kb on - strand, within HSERO_RS00815 at 169.535 kb on - strand at 169.535 kb on - strand at 169.537 kb on + strand at 169.537 kb on + strand at 169.545 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine remove 167,256 - HSERO_RS00795 0.59 -0.7 167,347 - HSERO_RS00795 0.68 -3.3 167,592 + -4.9 167,827 - HSERO_RS00800 0.32 -0.7 167,869 + HSERO_RS00800 0.41 -4.3 167,901 + HSERO_RS00800 0.48 -2.2 167,909 - HSERO_RS00800 0.50 -0.1 167,925 + HSERO_RS00800 0.54 -1.0 167,988 + HSERO_RS00800 0.68 -2.5 167,996 - HSERO_RS00800 0.69 +0.2 168,083 - HSERO_RS00800 0.89 -2.9 168,242 - -0.6 168,291 - +0.3 168,330 - HSERO_RS00805 0.16 +0.5 168,435 - HSERO_RS00805 0.37 +0.1 168,751 + -3.5 168,776 + +0.0 168,776 + +0.4 168,787 - +0.6 168,888 - HSERO_RS00810 0.34 -0.2 169,212 + HSERO_RS00815 0.38 -1.0 169,410 + HSERO_RS00815 0.86 -0.7 169,423 - HSERO_RS00815 0.89 +0.1 169,535 - -2.5 169,535 - -2.9 169,537 + -0.1 169,537 + -0.3 169,545 - -3.8
Or see this region's nucleotide sequence