Experiment: L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS00345 and HSERO_RS00350 overlap by 4 nucleotides HSERO_RS00350 and HSERO_RS00355 are separated by 80 nucleotides HSERO_RS00355 and HSERO_RS00360 are separated by 187 nucleotides HSERO_RS00360 and HSERO_RS00365 are separated by 170 nucleotides
HSERO_RS00345: HSERO_RS00345 - sensor histidine kinase, at 82,258 to 84,540
_RS00345
HSERO_RS00350: HSERO_RS00350 - hypothetical protein, at 84,537 to 85,103
_RS00350
HSERO_RS00355: HSERO_RS00355 - methyltransferase, at 85,184 to 86,503
_RS00355
HSERO_RS00360: HSERO_RS00360 - hypothetical protein, at 86,691 to 87,035
_RS00360
HSERO_RS00365: HSERO_RS00365 - membrane protein, at 87,206 to 87,751
_RS00365
Position (kb) 85
86
87 Strain fitness (log2 ratio) -1
0
1
2 at 84.428 kb on + strand at 85.258 kb on - strand at 85.258 kb on - strand at 85.392 kb on - strand, within HSERO_RS00355 at 85.431 kb on + strand, within HSERO_RS00355 at 85.458 kb on - strand, within HSERO_RS00355 at 85.491 kb on + strand, within HSERO_RS00355 at 85.566 kb on - strand, within HSERO_RS00355 at 85.648 kb on + strand, within HSERO_RS00355 at 85.753 kb on + strand, within HSERO_RS00355 at 85.861 kb on + strand, within HSERO_RS00355 at 85.986 kb on - strand, within HSERO_RS00355 at 85.992 kb on + strand, within HSERO_RS00355 at 86.016 kb on + strand, within HSERO_RS00355 at 86.065 kb on + strand, within HSERO_RS00355 at 86.108 kb on - strand, within HSERO_RS00355 at 86.304 kb on - strand, within HSERO_RS00355 at 86.377 kb on - strand at 86.698 kb on + strand at 86.849 kb on - strand, within HSERO_RS00360 at 87.083 kb on + strand at 87.083 kb on + strand at 87.091 kb on - strand at 87.422 kb on - strand, within HSERO_RS00365 at 87.494 kb on - strand, within HSERO_RS00365 at 87.494 kb on - strand, within HSERO_RS00365
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine remove 84,428 + -0.2 85,258 - +0.6 85,258 - -1.0 85,392 - HSERO_RS00355 0.16 -0.2 85,431 + HSERO_RS00355 0.19 -0.4 85,458 - HSERO_RS00355 0.21 +0.1 85,491 + HSERO_RS00355 0.23 +0.9 85,566 - HSERO_RS00355 0.29 +0.5 85,648 + HSERO_RS00355 0.35 +1.1 85,753 + HSERO_RS00355 0.43 +0.2 85,861 + HSERO_RS00355 0.51 +0.1 85,986 - HSERO_RS00355 0.61 +0.5 85,992 + HSERO_RS00355 0.61 +0.2 86,016 + HSERO_RS00355 0.63 +2.5 86,065 + HSERO_RS00355 0.67 -0.1 86,108 - HSERO_RS00355 0.70 +0.8 86,304 - HSERO_RS00355 0.85 -0.6 86,377 - -0.1 86,698 + -0.3 86,849 - HSERO_RS00360 0.46 -1.6 87,083 + +0.2 87,083 + -0.3 87,091 - +0.1 87,422 - HSERO_RS00365 0.40 +0.3 87,494 - HSERO_RS00365 0.53 -0.9 87,494 - HSERO_RS00365 0.53 -0.5
Or see this region's nucleotide sequence