Experiment: L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS00325 and HSERO_RS00330 overlap by 4 nucleotides HSERO_RS00330 and HSERO_RS00335 are separated by 128 nucleotides HSERO_RS00335 and HSERO_RS00340 are separated by 431 nucleotides
HSERO_RS00325: HSERO_RS00325 - multidrug DMT transporter permease, at 79,037 to 79,882
_RS00325
HSERO_RS00330: HSERO_RS00330 - SAM-dependent methyltransferase, at 79,879 to 80,556
_RS00330
HSERO_RS00335: HSERO_RS00335 - hypothetical protein, at 80,685 to 80,942
_RS00335
HSERO_RS00340: HSERO_RS00340 - XRE family transcriptional regulator, at 81,374 to 82,123
_RS00340
Position (kb) 80
81 Strain fitness (log2 ratio) -1
0
1 at 79.717 kb on + strand at 79.725 kb on - strand, within HSERO_RS00325 at 79.809 kb on + strand at 79.809 kb on + strand at 79.809 kb on + strand at 79.856 kb on - strand at 79.959 kb on + strand, within HSERO_RS00330 at 80.050 kb on - strand at 80.172 kb on - strand, within HSERO_RS00330 at 80.240 kb on + strand, within HSERO_RS00330 at 80.264 kb on - strand, within HSERO_RS00330 at 80.430 kb on + strand, within HSERO_RS00330 at 80.464 kb on - strand, within HSERO_RS00330 at 80.475 kb on - strand, within HSERO_RS00330 at 80.524 kb on + strand at 80.532 kb on - strand at 80.539 kb on + strand at 80.681 kb on - strand at 80.691 kb on - strand at 80.908 kb on + strand, within HSERO_RS00335 at 80.908 kb on + strand, within HSERO_RS00335 at 80.908 kb on + strand, within HSERO_RS00335 at 80.916 kb on - strand, within HSERO_RS00335 at 80.916 kb on - strand, within HSERO_RS00335 at 80.944 kb on - strand at 81.164 kb on + strand at 81.220 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine remove 79,717 + -0.1 79,725 - HSERO_RS00325 0.81 -0.4 79,809 + -0.2 79,809 + +0.2 79,809 + -1.0 79,856 - +1.1 79,959 + HSERO_RS00330 0.12 -0.4 80,050 - -0.4 80,172 - HSERO_RS00330 0.43 -0.3 80,240 + HSERO_RS00330 0.53 +0.8 80,264 - HSERO_RS00330 0.57 -0.1 80,430 + HSERO_RS00330 0.81 -0.2 80,464 - HSERO_RS00330 0.86 -1.0 80,475 - HSERO_RS00330 0.88 -0.2 80,524 + +0.4 80,532 - -0.0 80,539 + +0.7 80,681 - -0.4 80,691 - +0.5 80,908 + HSERO_RS00335 0.86 +0.4 80,908 + HSERO_RS00335 0.86 +0.7 80,908 + HSERO_RS00335 0.86 +0.4 80,916 - HSERO_RS00335 0.90 -0.1 80,916 - HSERO_RS00335 0.90 -0.1 80,944 - -0.2 81,164 + -0.5 81,220 - +1.1
Or see this region's nucleotide sequence