Experiment: R2A with silver chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS03355 and LRK54_RS03360 are separated by 75 nucleotides LRK54_RS03360 and LRK54_RS03365 overlap by 17 nucleotides LRK54_RS03365 and LRK54_RS03370 are separated by 64 nucleotides LRK54_RS03370 and LRK54_RS03375 are separated by 238 nucleotides
LRK54_RS03355: LRK54_RS03355 - tRNA lysidine(34) synthetase TilS, at 722,600 to 723,904
_RS03355
LRK54_RS03360: LRK54_RS03360 - exodeoxyribonuclease VII small subunit, at 723,980 to 724,237
_RS03360
LRK54_RS03365: LRK54_RS03365 - polyprenyl synthetase family protein, at 724,221 to 725,120
_RS03365
LRK54_RS03370: LRK54_RS03370 - DUF4870 domain-containing protein, at 725,185 to 725,586
_RS03370
LRK54_RS03375: LRK54_RS03375 - metalloprotease PmbA, at 725,825 to 727,192
_RS03375
Position (kb)
724
725
726 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 724.311 kb on + strand, within LRK54_RS03365 at 724.311 kb on + strand, within LRK54_RS03365 at 724.311 kb on + strand, within LRK54_RS03365 at 724.312 kb on - strand, within LRK54_RS03365 at 724.312 kb on - strand, within LRK54_RS03365 at 724.312 kb on - strand, within LRK54_RS03365 at 724.312 kb on - strand, within LRK54_RS03365 at 724.312 kb on - strand, within LRK54_RS03365 at 724.344 kb on + strand, within LRK54_RS03365 at 724.345 kb on - strand, within LRK54_RS03365 at 724.345 kb on - strand, within LRK54_RS03365 at 724.389 kb on + strand, within LRK54_RS03365 at 724.389 kb on + strand, within LRK54_RS03365 at 724.390 kb on - strand, within LRK54_RS03365 at 724.390 kb on - strand, within LRK54_RS03365 at 724.390 kb on - strand, within LRK54_RS03365 at 724.390 kb on - strand, within LRK54_RS03365 at 724.390 kb on - strand, within LRK54_RS03365 at 724.467 kb on + strand, within LRK54_RS03365 at 724.467 kb on + strand, within LRK54_RS03365 at 724.467 kb on + strand, within LRK54_RS03365 at 724.467 kb on + strand, within LRK54_RS03365 at 724.467 kb on + strand, within LRK54_RS03365 at 724.467 kb on + strand, within LRK54_RS03365 at 724.468 kb on - strand, within LRK54_RS03365 at 724.468 kb on - strand, within LRK54_RS03365 at 724.468 kb on - strand, within LRK54_RS03365 at 725.098 kb on - strand at 725.098 kb on - strand at 725.115 kb on + strand at 725.115 kb on + strand at 725.116 kb on - strand at 725.116 kb on - strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.220 kb on - strand at 725.220 kb on - strand at 725.220 kb on - strand at 725.220 kb on - strand at 725.220 kb on - strand at 725.220 kb on - strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.811 kb on + strand at 725.811 kb on + strand at 725.811 kb on + strand at 725.811 kb on + strand at 725.811 kb on + strand at 725.812 kb on - strand at 725.812 kb on - strand at 725.812 kb on - strand at 725.812 kb on - strand at 725.823 kb on + strand at 725.823 kb on + strand at 725.823 kb on + strand at 725.823 kb on + strand at 725.823 kb on + strand at 725.823 kb on + strand at 725.823 kb on + strand at 725.823 kb on + strand at 725.824 kb on - strand at 725.824 kb on - strand at 725.824 kb on - strand at 725.824 kb on - strand at 725.824 kb on - strand at 725.824 kb on - strand at 725.824 kb on - strand at 725.824 kb on - strand at 725.826 kb on + strand at 725.826 kb on + strand at 725.826 kb on + strand at 725.826 kb on + strand at 725.826 kb on + strand at 725.826 kb on + strand at 725.826 kb on + strand at 725.826 kb on + strand at 725.826 kb on + strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.967 kb on + strand, within LRK54_RS03375 at 726.015 kb on + strand, within LRK54_RS03375 at 726.015 kb on + strand, within LRK54_RS03375 at 726.016 kb on - strand, within LRK54_RS03375 at 726.016 kb on - strand, within LRK54_RS03375 at 726.016 kb on - strand, within LRK54_RS03375
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with silver chloride 1 mM remove 724,311 + LRK54_RS03365 0.10 -1.8 724,311 + LRK54_RS03365 0.10 -1.1 724,311 + LRK54_RS03365 0.10 -0.3 724,312 - LRK54_RS03365 0.10 -1.1 724,312 - LRK54_RS03365 0.10 +1.5 724,312 - LRK54_RS03365 0.10 -0.8 724,312 - LRK54_RS03365 0.10 -1.4 724,312 - LRK54_RS03365 0.10 -0.3 724,344 + LRK54_RS03365 0.14 +1.4 724,345 - LRK54_RS03365 0.14 +0.3 724,345 - LRK54_RS03365 0.14 -1.7 724,389 + LRK54_RS03365 0.19 +0.5 724,389 + LRK54_RS03365 0.19 -2.6 724,390 - LRK54_RS03365 0.19 +0.0 724,390 - LRK54_RS03365 0.19 -1.1 724,390 - LRK54_RS03365 0.19 -2.1 724,390 - LRK54_RS03365 0.19 +0.8 724,390 - LRK54_RS03365 0.19 -1.0 724,467 + LRK54_RS03365 0.27 -0.7 724,467 + LRK54_RS03365 0.27 +0.5 724,467 + LRK54_RS03365 0.27 -1.0 724,467 + LRK54_RS03365 0.27 +2.0 724,467 + LRK54_RS03365 0.27 +2.0 724,467 + LRK54_RS03365 0.27 -0.6 724,468 - LRK54_RS03365 0.27 +0.3 724,468 - LRK54_RS03365 0.27 -1.4 724,468 - LRK54_RS03365 0.27 +0.0 725,098 - -0.9 725,098 - -0.7 725,115 + -0.4 725,115 + -1.3 725,116 - +0.0 725,116 - +1.8 725,219 + -1.5 725,219 + -0.8 725,219 + -1.2 725,219 + -1.3 725,219 + +2.0 725,219 + +0.6 725,219 + -0.8 725,219 + +1.9 725,219 + -2.8 725,219 + -0.5 725,219 + -0.3 725,219 + +0.5 725,220 - -1.5 725,220 - -0.5 725,220 - -0.1 725,220 - -0.4 725,220 - -0.9 725,220 - -0.3 725,600 + +0.4 725,600 + -1.0 725,600 + +1.4 725,600 + -0.8 725,600 + +0.2 725,600 + +0.6 725,600 + +0.8 725,600 + -1.0 725,600 + -1.7 725,600 + -0.0 725,600 + +1.0 725,600 + +1.0 725,601 - -2.0 725,601 - +2.3 725,601 - -0.3 725,601 - -1.0 725,601 - -0.5 725,601 - +0.3 725,601 - -1.2 725,601 - +0.5 725,601 - +0.9 725,601 - -1.0 725,601 - -0.0 725,601 - -1.0 725,653 + -0.8 725,653 + -1.0 725,653 + +0.0 725,653 + -1.0 725,653 + -0.2 725,653 + -0.5 725,653 + -0.5 725,653 + -1.5 725,653 + +1.1 725,653 + +0.0 725,653 + +0.2 725,653 + -1.7 725,653 + +0.2 725,653 + -1.8 725,653 + +0.5 725,653 + +0.1 725,653 + +1.0 725,653 + -0.5 725,654 - -0.2 725,654 - -0.9 725,654 - +2.3 725,654 - +2.1 725,654 - -0.9 725,654 - -0.3 725,654 - +2.0 725,654 - +2.4 725,654 - +2.3 725,654 - +0.5 725,654 - +0.2 725,654 - +0.6 725,654 - -1.1 725,811 + +2.0 725,811 + -1.3 725,811 + +0.0 725,811 + -1.1 725,811 + -2.5 725,812 - +0.2 725,812 - -0.2 725,812 - -1.8 725,812 - +0.2 725,823 + -1.0 725,823 + +2.0 725,823 + -0.3 725,823 + -1.0 725,823 + -0.5 725,823 + -0.9 725,823 + -0.9 725,823 + +1.8 725,824 - -0.3 725,824 - -2.4 725,824 - -0.8 725,824 - +0.2 725,824 - +1.0 725,824 - -0.2 725,824 - -0.9 725,824 - +0.4 725,826 + -0.0 725,826 + +2.0 725,826 + +0.4 725,826 + -0.5 725,826 + +1.0 725,826 + -0.5 725,826 + -1.9 725,826 + +2.0 725,826 + -1.0 725,827 - -0.3 725,827 - +0.6 725,827 - +0.6 725,827 - +0.3 725,827 - +0.5 725,827 - -1.3 725,827 - +0.6 725,827 - -0.5 725,827 - +0.8 725,827 - +0.0 725,967 + LRK54_RS03375 0.10 -3.4 726,015 + LRK54_RS03375 0.14 -2.4 726,015 + LRK54_RS03375 0.14 -1.8 726,016 - LRK54_RS03375 0.14 +1.6 726,016 - LRK54_RS03375 0.14 -2.4 726,016 - LRK54_RS03375 0.14 -1.3
Or see this region's nucleotide sequence