Experiment: Recovery broth with 3-Acetylpyridine 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12410 and BBR_RS12415 are separated by 125 nucleotides BBR_RS12415 and BBR_RS12420 are separated by 268 nucleotides BBR_RS12420 and BBR_RS12425 are separated by 91 nucleotides BBR_RS12425 and BBR_RS12430 are separated by 358 nucleotides
BBR_RS12410: BBR_RS12410 - non-canonical purine NTP pyrophosphatase, at 533,054 to 533,827
_RS12410
BBR_RS12415: BBR_RS12415 - permease, at 533,953 to 534,954
_RS12415
BBR_RS12420: BBR_RS12420 - tRNA-Leu, at 535,223 to 535,303
_RS12420
BBR_RS12425: BBR_RS12425 - tRNA-Thr, at 535,395 to 535,470
_RS12425
BBR_RS12430: BBR_RS12430 - glucose-6-phosphate isomerase, at 535,829 to 537,529
_RS12430
Position (kb) 533
534
535 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 532.959 kb on - strand at 532.959 kb on - strand at 532.961 kb on + strand at 532.963 kb on - strand at 532.972 kb on + strand at 532.987 kb on - strand at 532.995 kb on - strand at 532.995 kb on - strand at 533.004 kb on + strand at 533.009 kb on + strand at 533.012 kb on - strand at 533.062 kb on - strand at 533.128 kb on - strand at 533.147 kb on + strand, within BBR_RS12410 at 533.202 kb on - strand, within BBR_RS12410 at 533.221 kb on + strand, within BBR_RS12410 at 533.221 kb on + strand, within BBR_RS12410 at 533.221 kb on + strand, within BBR_RS12410 at 533.229 kb on - strand, within BBR_RS12410 at 533.237 kb on + strand, within BBR_RS12410 at 533.237 kb on + strand, within BBR_RS12410 at 533.274 kb on + strand, within BBR_RS12410 at 533.274 kb on + strand, within BBR_RS12410 at 533.346 kb on + strand, within BBR_RS12410 at 533.364 kb on - strand, within BBR_RS12410 at 533.364 kb on - strand, within BBR_RS12410 at 533.369 kb on - strand, within BBR_RS12410 at 533.369 kb on - strand, within BBR_RS12410 at 533.372 kb on - strand, within BBR_RS12410 at 533.392 kb on - strand, within BBR_RS12410 at 533.443 kb on - strand, within BBR_RS12410 at 533.451 kb on + strand, within BBR_RS12410 at 533.461 kb on - strand, within BBR_RS12410 at 533.514 kb on - strand, within BBR_RS12410 at 533.521 kb on + strand, within BBR_RS12410 at 533.583 kb on + strand, within BBR_RS12410 at 533.585 kb on + strand, within BBR_RS12410 at 533.585 kb on + strand, within BBR_RS12410 at 533.645 kb on + strand, within BBR_RS12410 at 533.689 kb on + strand, within BBR_RS12410 at 533.695 kb on + strand, within BBR_RS12410 at 533.697 kb on - strand, within BBR_RS12410 at 533.707 kb on + strand, within BBR_RS12410 at 533.713 kb on + strand, within BBR_RS12410 at 533.720 kb on + strand, within BBR_RS12410 at 533.720 kb on + strand, within BBR_RS12410 at 533.720 kb on + strand, within BBR_RS12410 at 533.725 kb on + strand, within BBR_RS12410 at 533.728 kb on - strand, within BBR_RS12410 at 533.782 kb on + strand at 533.824 kb on + strand at 533.853 kb on + strand at 533.856 kb on - strand at 533.859 kb on - strand at 533.859 kb on - strand at 533.859 kb on - strand at 533.859 kb on - strand at 533.860 kb on + strand at 533.860 kb on + strand at 533.860 kb on + strand at 533.860 kb on + strand at 533.868 kb on - strand at 533.868 kb on - strand at 533.913 kb on + strand at 533.921 kb on + strand at 533.938 kb on + strand at 533.944 kb on - strand at 533.952 kb on + strand at 533.952 kb on + strand at 533.986 kb on + strand at 534.032 kb on + strand at 534.032 kb on + strand at 534.040 kb on - strand at 534.043 kb on + strand at 534.043 kb on + strand at 534.043 kb on + strand at 534.051 kb on - strand at 534.051 kb on - strand at 534.051 kb on - strand at 534.055 kb on + strand, within BBR_RS12415 at 534.138 kb on + strand, within BBR_RS12415 at 534.154 kb on - strand, within BBR_RS12415 at 534.167 kb on + strand, within BBR_RS12415 at 534.172 kb on + strand, within BBR_RS12415 at 534.180 kb on - strand, within BBR_RS12415 at 534.190 kb on - strand, within BBR_RS12415 at 534.206 kb on + strand, within BBR_RS12415 at 534.206 kb on + strand, within BBR_RS12415 at 534.214 kb on - strand, within BBR_RS12415 at 534.214 kb on - strand, within BBR_RS12415 at 534.214 kb on - strand, within BBR_RS12415 at 534.214 kb on - strand, within BBR_RS12415 at 534.226 kb on - strand, within BBR_RS12415 at 534.226 kb on - strand, within BBR_RS12415 at 534.226 kb on - strand, within BBR_RS12415 at 534.226 kb on - strand, within BBR_RS12415 at 534.248 kb on + strand, within BBR_RS12415 at 534.248 kb on + strand, within BBR_RS12415 at 534.283 kb on + strand, within BBR_RS12415 at 534.291 kb on - strand, within BBR_RS12415 at 534.298 kb on + strand, within BBR_RS12415 at 534.298 kb on + strand, within BBR_RS12415 at 534.306 kb on - strand, within BBR_RS12415 at 534.311 kb on - strand, within BBR_RS12415 at 534.334 kb on - strand, within BBR_RS12415 at 534.355 kb on - strand, within BBR_RS12415 at 534.357 kb on + strand, within BBR_RS12415 at 534.372 kb on + strand, within BBR_RS12415 at 534.375 kb on - strand, within BBR_RS12415 at 534.380 kb on - strand, within BBR_RS12415 at 534.380 kb on - strand, within BBR_RS12415 at 534.403 kb on + strand, within BBR_RS12415 at 534.403 kb on + strand, within BBR_RS12415 at 534.409 kb on - strand, within BBR_RS12415 at 534.436 kb on - strand, within BBR_RS12415 at 534.444 kb on - strand, within BBR_RS12415 at 534.455 kb on + strand, within BBR_RS12415 at 534.463 kb on - strand, within BBR_RS12415 at 534.463 kb on - strand, within BBR_RS12415 at 534.474 kb on - strand, within BBR_RS12415 at 534.590 kb on + strand, within BBR_RS12415 at 534.598 kb on - strand, within BBR_RS12415 at 534.614 kb on + strand, within BBR_RS12415 at 534.624 kb on + strand, within BBR_RS12415 at 534.624 kb on + strand, within BBR_RS12415 at 534.662 kb on + strand, within BBR_RS12415 at 534.665 kb on + strand, within BBR_RS12415 at 534.670 kb on - strand, within BBR_RS12415 at 534.686 kb on - strand, within BBR_RS12415 at 534.686 kb on - strand, within BBR_RS12415 at 534.700 kb on - strand, within BBR_RS12415 at 534.702 kb on + strand, within BBR_RS12415 at 534.733 kb on + strand, within BBR_RS12415 at 534.733 kb on + strand, within BBR_RS12415 at 534.767 kb on + strand, within BBR_RS12415 at 534.769 kb on + strand, within BBR_RS12415 at 534.770 kb on - strand, within BBR_RS12415 at 534.770 kb on - strand, within BBR_RS12415 at 534.777 kb on + strand, within BBR_RS12415 at 534.813 kb on + strand, within BBR_RS12415 at 534.833 kb on + strand, within BBR_RS12415 at 534.833 kb on + strand, within BBR_RS12415 at 534.833 kb on + strand, within BBR_RS12415 at 534.841 kb on - strand, within BBR_RS12415 at 534.848 kb on - strand, within BBR_RS12415 at 534.890 kb on - strand at 534.903 kb on - strand at 534.908 kb on - strand at 534.948 kb on - strand at 534.950 kb on - strand at 534.963 kb on + strand at 535.023 kb on + strand at 535.031 kb on + strand at 535.031 kb on - strand at 535.063 kb on - strand at 535.157 kb on + strand at 535.210 kb on - strand at 535.211 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.316 kb on + strand at 535.319 kb on - strand at 535.391 kb on - strand at 535.391 kb on - strand at 535.391 kb on - strand at 535.633 kb on + strand at 535.641 kb on - strand at 535.668 kb on + strand at 535.676 kb on - strand at 535.678 kb on - strand at 535.696 kb on - strand at 535.775 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with 3-Acetylpyridine 0.04 mM remove 532,959 - -1.5 532,959 - -1.3 532,961 + -0.4 532,963 - -1.2 532,972 + -0.9 532,987 - -0.5 532,995 - -1.8 532,995 - +2.3 533,004 + -1.6 533,009 + -2.3 533,012 - +2.1 533,062 - -2.3 533,128 - -1.7 533,147 + BBR_RS12410 0.12 -1.8 533,202 - BBR_RS12410 0.19 -2.5 533,221 + BBR_RS12410 0.22 +3.3 533,221 + BBR_RS12410 0.22 -1.9 533,221 + BBR_RS12410 0.22 -1.1 533,229 - BBR_RS12410 0.23 -1.6 533,237 + BBR_RS12410 0.24 +3.0 533,237 + BBR_RS12410 0.24 +3.3 533,274 + BBR_RS12410 0.28 -1.0 533,274 + BBR_RS12410 0.28 -1.9 533,346 + BBR_RS12410 0.38 -1.1 533,364 - BBR_RS12410 0.40 -0.9 533,364 - BBR_RS12410 0.40 -1.5 533,369 - BBR_RS12410 0.41 +0.5 533,369 - BBR_RS12410 0.41 -0.6 533,372 - BBR_RS12410 0.41 -1.3 533,392 - BBR_RS12410 0.44 +0.7 533,443 - BBR_RS12410 0.50 +0.4 533,451 + BBR_RS12410 0.51 -0.9 533,461 - BBR_RS12410 0.53 -0.7 533,514 - BBR_RS12410 0.59 -1.6 533,521 + BBR_RS12410 0.60 +2.0 533,583 + BBR_RS12410 0.68 -0.9 533,585 + BBR_RS12410 0.69 -1.6 533,585 + BBR_RS12410 0.69 +1.5 533,645 + BBR_RS12410 0.76 -1.4 533,689 + BBR_RS12410 0.82 +1.6 533,695 + BBR_RS12410 0.83 -1.4 533,697 - BBR_RS12410 0.83 -1.3 533,707 + BBR_RS12410 0.84 -1.3 533,713 + BBR_RS12410 0.85 -1.2 533,720 + BBR_RS12410 0.86 -0.1 533,720 + BBR_RS12410 0.86 -1.1 533,720 + BBR_RS12410 0.86 +1.5 533,725 + BBR_RS12410 0.87 +2.6 533,728 - BBR_RS12410 0.87 -1.3 533,782 + +2.6 533,824 + +3.0 533,853 + +1.1 533,856 - -1.6 533,859 - -1.3 533,859 - +0.7 533,859 - +2.3 533,859 - +1.9 533,860 + -0.3 533,860 + +0.4 533,860 + -2.7 533,860 + -2.0 533,868 - +2.5 533,868 - -2.1 533,913 + +3.5 533,921 + -1.9 533,938 + -0.8 533,944 - +4.3 533,952 + -1.1 533,952 + +0.4 533,986 + -0.3 534,032 + +2.2 534,032 + +2.5 534,040 - +1.3 534,043 + +0.1 534,043 + +2.2 534,043 + +2.1 534,051 - +2.7 534,051 - -2.2 534,051 - +2.2 534,055 + BBR_RS12415 0.10 +0.1 534,138 + BBR_RS12415 0.18 +1.2 534,154 - BBR_RS12415 0.20 -0.2 534,167 + BBR_RS12415 0.21 -0.2 534,172 + BBR_RS12415 0.22 -1.7 534,180 - BBR_RS12415 0.23 +1.5 534,190 - BBR_RS12415 0.24 +2.5 534,206 + BBR_RS12415 0.25 +0.7 534,206 + BBR_RS12415 0.25 -1.3 534,214 - BBR_RS12415 0.26 -0.9 534,214 - BBR_RS12415 0.26 -3.0 534,214 - BBR_RS12415 0.26 +2.4 534,214 - BBR_RS12415 0.26 +2.6 534,226 - BBR_RS12415 0.27 -1.8 534,226 - BBR_RS12415 0.27 +0.1 534,226 - BBR_RS12415 0.27 -1.9 534,226 - BBR_RS12415 0.27 +2.5 534,248 + BBR_RS12415 0.29 -1.5 534,248 + BBR_RS12415 0.29 -1.9 534,283 + BBR_RS12415 0.33 +1.2 534,291 - BBR_RS12415 0.34 -3.3 534,298 + BBR_RS12415 0.34 +0.2 534,298 + BBR_RS12415 0.34 -0.4 534,306 - BBR_RS12415 0.35 -1.9 534,311 - BBR_RS12415 0.36 -2.7 534,334 - BBR_RS12415 0.38 -3.1 534,355 - BBR_RS12415 0.40 -2.1 534,357 + BBR_RS12415 0.40 -1.6 534,372 + BBR_RS12415 0.42 -2.1 534,375 - BBR_RS12415 0.42 +2.5 534,380 - BBR_RS12415 0.43 -1.7 534,380 - BBR_RS12415 0.43 +2.0 534,403 + BBR_RS12415 0.45 +1.3 534,403 + BBR_RS12415 0.45 +2.2 534,409 - BBR_RS12415 0.46 +0.7 534,436 - BBR_RS12415 0.48 +0.9 534,444 - BBR_RS12415 0.49 +1.8 534,455 + BBR_RS12415 0.50 +4.0 534,463 - BBR_RS12415 0.51 +1.7 534,463 - BBR_RS12415 0.51 -2.0 534,474 - BBR_RS12415 0.52 -0.1 534,590 + BBR_RS12415 0.64 +2.1 534,598 - BBR_RS12415 0.64 +2.6 534,614 + BBR_RS12415 0.66 +1.9 534,624 + BBR_RS12415 0.67 -0.2 534,624 + BBR_RS12415 0.67 +2.8 534,662 + BBR_RS12415 0.71 +1.9 534,665 + BBR_RS12415 0.71 +2.7 534,670 - BBR_RS12415 0.72 +1.5 534,686 - BBR_RS12415 0.73 -2.9 534,686 - BBR_RS12415 0.73 -0.8 534,700 - BBR_RS12415 0.75 -1.9 534,702 + BBR_RS12415 0.75 -1.9 534,733 + BBR_RS12415 0.78 -0.9 534,733 + BBR_RS12415 0.78 -1.4 534,767 + BBR_RS12415 0.81 +1.0 534,769 + BBR_RS12415 0.81 -0.3 534,770 - BBR_RS12415 0.82 +0.4 534,770 - BBR_RS12415 0.82 -2.7 534,777 + BBR_RS12415 0.82 -1.4 534,813 + BBR_RS12415 0.86 +1.8 534,833 + BBR_RS12415 0.88 +2.5 534,833 + BBR_RS12415 0.88 +3.0 534,833 + BBR_RS12415 0.88 +2.0 534,841 - BBR_RS12415 0.89 +4.4 534,848 - BBR_RS12415 0.89 +1.8 534,890 - +0.8 534,903 - -0.9 534,908 - -1.5 534,948 - +2.1 534,950 - +0.5 534,963 + +1.3 535,023 + +2.0 535,031 + -1.0 535,031 - -2.4 535,063 - +2.6 535,157 + -1.3 535,210 - -0.5 535,211 + -2.2 535,212 + +2.1 535,212 + -1.6 535,212 + -0.5 535,212 + -1.1 535,212 + +3.0 535,212 + -1.4 535,212 + -1.3 535,212 + +1.0 535,212 + +2.4 535,212 + -2.1 535,212 + +2.2 535,220 - -2.0 535,220 - +3.2 535,220 - +2.9 535,220 - +1.7 535,220 - +2.3 535,220 - -0.9 535,220 - +1.6 535,316 + -0.1 535,319 - -1.2 535,391 - -0.2 535,391 - -2.1 535,391 - -0.7 535,633 + -1.6 535,641 - -1.0 535,668 + +1.0 535,676 - +1.2 535,678 - +1.3 535,696 - -3.0 535,775 + +1.1
Or see this region's nucleotide sequence