Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3677 and Dshi_3678 are separated by 116 nucleotides Dshi_3678 and Dshi_3679 overlap by 4 nucleotides Dshi_3679 and Dshi_3680 are separated by 138 nucleotides
Dshi_3677: Dshi_3677 - hypothetical protein (RefSeq), at 80,378 to 80,902
_3677
Dshi_3678: Dshi_3678 - ATP-binding protein (RefSeq), at 81,019 to 81,843
_3678
Dshi_3679: Dshi_3679 - Integrase catalytic region (RefSeq), at 81,840 to 83,333
_3679
Dshi_3680: Dshi_3680 - Resolvase domain (RefSeq), at 83,472 to 84,071
_3680
Position (kb)
81
82
83
84 Strain fitness (log2 ratio)
-1
0
1 at 80.851 kb on + strand at 80.851 kb on - strand at 80.860 kb on + strand at 80.862 kb on + strand at 80.867 kb on + strand at 80.875 kb on - strand at 80.879 kb on + strand at 80.884 kb on + strand at 80.884 kb on + strand at 80.887 kb on - strand at 80.892 kb on - strand at 80.892 kb on - strand at 80.892 kb on - strand at 80.892 kb on - strand at 80.892 kb on - strand at 80.892 kb on - strand at 80.892 kb on - strand at 80.892 kb on - strand at 80.892 kb on - strand at 80.892 kb on - strand at 80.892 kb on - strand at 80.892 kb on - strand at 80.892 kb on - strand at 80.892 kb on - strand at 80.892 kb on - strand at 80.899 kb on + strand at 80.901 kb on + strand at 80.998 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 80,851 + -0.8 80,851 - +1.2 80,860 + +0.7 80,862 + -0.2 80,867 + -0.2 80,875 - -0.2 80,879 + -1.0 80,884 + +0.4 80,884 + -0.2 80,887 - -0.1 80,892 - -0.4 80,892 - -0.1 80,892 - -0.2 80,892 - +0.3 80,892 - +0.0 80,892 - +0.1 80,892 - -0.3 80,892 - +0.5 80,892 - -0.1 80,892 - -0.4 80,892 - -0.5 80,892 - +0.1 80,892 - -0.5 80,892 - -0.2 80,892 - -1.4 80,899 + -0.5 80,901 + -0.8 80,998 - -1.3
Or see this region's nucleotide sequence