Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0793 and Dshi_0794 are separated by 145 nucleotides Dshi_0794 and Dshi_0795 are separated by 1 nucleotides Dshi_0795 and Dshi_0796 are separated by 190 nucleotides
Dshi_0793: Dshi_0793 - O-acetylhomoserine/O-acetylserine sulfhydrylase (RefSeq), at 800,127 to 801,422
_0793
Dshi_0794: Dshi_0794 - Imidazoleglycerol-phosphate dehydratase (RefSeq), at 801,568 to 802,155
_0794
Dshi_0795: Dshi_0795 - imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (RefSeq), at 802,157 to 802,795
_0795
Dshi_0796: Dshi_0796 - ornithine carbamoyltransferase (RefSeq), at 802,986 to 803,912
_0796
Position (kb) 802
803 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 801.224 kb on + strand, within Dshi_0793 at 801.231 kb on - strand, within Dshi_0793 at 801.306 kb on + strand at 801.314 kb on - strand at 801.381 kb on - strand at 801.397 kb on + strand at 801.425 kb on - strand at 802.803 kb on + strand at 802.826 kb on - strand at 802.878 kb on + strand at 803.137 kb on - strand, within Dshi_0796 at 803.385 kb on - strand, within Dshi_0796 at 803.387 kb on + strand, within Dshi_0796 at 803.410 kb on + strand, within Dshi_0796 at 803.502 kb on - strand, within Dshi_0796 at 803.601 kb on + strand, within Dshi_0796 at 803.781 kb on + strand, within Dshi_0796
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 801,224 + Dshi_0793 0.85 -1.4 801,231 - Dshi_0793 0.85 -0.3 801,306 + -0.2 801,314 - +0.2 801,381 - +0.1 801,397 + -1.0 801,425 - +0.2 802,803 + +0.0 802,826 - +0.6 802,878 + +0.4 803,137 - Dshi_0796 0.16 -1.4 803,385 - Dshi_0796 0.43 -3.8 803,387 + Dshi_0796 0.43 -3.4 803,410 + Dshi_0796 0.46 -3.2 803,502 - Dshi_0796 0.56 -3.2 803,601 + Dshi_0796 0.66 -3.1 803,781 + Dshi_0796 0.86 -3.5
Or see this region's nucleotide sequence