Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS20320 and HSERO_RS20325 are separated by 76 nucleotides HSERO_RS20325 and HSERO_RS20330 are separated by 61 nucleotides HSERO_RS20330 and HSERO_RS20335 are separated by 73 nucleotides
HSERO_RS20320: HSERO_RS20320 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 4,657,853 to 4,658,650
_RS20320
HSERO_RS20325: HSERO_RS20325 - IGP synthase, amidotransferase subunit (EC 4.3.2.10) (from data) , at 4,658,727 to 4,659,365
_RS20325
HSERO_RS20330: HSERO_RS20330 - imidazoleglycerol-phosphate dehydratase, at 4,659,427 to 4,660,023
_RS20330
HSERO_RS20335: HSERO_RS20335 - histidinol-phosphate aminotransferase, at 4,660,097 to 4,661,215
_RS20335
Position (kb)
4659
4660
4661 Strain fitness (log2 ratio)
-1
0
1
2 at 4658.527 kb on + strand, within HSERO_RS20320 at 4658.665 kb on - strand at 4658.848 kb on + strand, within HSERO_RS20325 at 4659.000 kb on - strand, within HSERO_RS20325 at 4659.000 kb on - strand, within HSERO_RS20325 at 4659.015 kb on - strand, within HSERO_RS20325 at 4659.076 kb on + strand, within HSERO_RS20325 at 4659.127 kb on - strand, within HSERO_RS20325 at 4659.127 kb on - strand, within HSERO_RS20325 at 4659.154 kb on - strand, within HSERO_RS20325 at 4659.510 kb on - strand, within HSERO_RS20330 at 4659.529 kb on - strand, within HSERO_RS20330 at 4659.564 kb on + strand, within HSERO_RS20330 at 4659.613 kb on + strand, within HSERO_RS20330 at 4659.626 kb on - strand, within HSERO_RS20330 at 4659.784 kb on + strand, within HSERO_RS20330 at 4659.868 kb on + strand, within HSERO_RS20330 at 4659.948 kb on + strand, within HSERO_RS20330 at 4660.022 kb on - strand at 4660.029 kb on + strand at 4660.041 kb on + strand at 4660.047 kb on - strand at 4660.059 kb on + strand at 4660.072 kb on - strand at 4660.072 kb on - strand at 4660.077 kb on - strand at 4660.136 kb on - strand at 4660.186 kb on - strand at 4660.309 kb on - strand, within HSERO_RS20335 at 4660.424 kb on - strand, within HSERO_RS20335 at 4660.437 kb on - strand, within HSERO_RS20335 at 4660.938 kb on - strand, within HSERO_RS20335 at 4660.952 kb on + strand, within HSERO_RS20335 at 4660.976 kb on + strand, within HSERO_RS20335 at 4660.976 kb on + strand, within HSERO_RS20335 at 4661.014 kb on + strand, within HSERO_RS20335
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 4,658,527 + HSERO_RS20320 0.84 -0.5 4,658,665 - +1.4 4,658,848 + HSERO_RS20325 0.19 +1.3 4,659,000 - HSERO_RS20325 0.43 +0.3 4,659,000 - HSERO_RS20325 0.43 -0.2 4,659,015 - HSERO_RS20325 0.45 +0.0 4,659,076 + HSERO_RS20325 0.55 +1.6 4,659,127 - HSERO_RS20325 0.63 +0.3 4,659,127 - HSERO_RS20325 0.63 +0.7 4,659,154 - HSERO_RS20325 0.67 +0.4 4,659,510 - HSERO_RS20330 0.14 -0.1 4,659,529 - HSERO_RS20330 0.17 +0.4 4,659,564 + HSERO_RS20330 0.23 +1.1 4,659,613 + HSERO_RS20330 0.31 +0.6 4,659,626 - HSERO_RS20330 0.33 -0.3 4,659,784 + HSERO_RS20330 0.60 +0.7 4,659,868 + HSERO_RS20330 0.74 +1.2 4,659,948 + HSERO_RS20330 0.87 -0.1 4,660,022 - -0.1 4,660,029 + +0.5 4,660,041 + +0.0 4,660,047 - +0.6 4,660,059 + +0.6 4,660,072 - -1.2 4,660,072 - +1.8 4,660,077 - -1.6 4,660,136 - +1.6 4,660,186 - +0.9 4,660,309 - HSERO_RS20335 0.19 +0.4 4,660,424 - HSERO_RS20335 0.29 -0.2 4,660,437 - HSERO_RS20335 0.30 +0.2 4,660,938 - HSERO_RS20335 0.75 +0.1 4,660,952 + HSERO_RS20335 0.76 +0.3 4,660,976 + HSERO_RS20335 0.79 +0.5 4,660,976 + HSERO_RS20335 0.79 +0.7 4,661,014 + HSERO_RS20335 0.82 +0.6
Or see this region's nucleotide sequence