Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS20310 and HSERO_RS20315 overlap by 4 nucleotides HSERO_RS20315 and HSERO_RS20320 are separated by 1 nucleotides HSERO_RS20320 and HSERO_RS20325 are separated by 76 nucleotides HSERO_RS20325 and HSERO_RS20330 are separated by 61 nucleotides
HSERO_RS20310: HSERO_RS20310 - phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) (from data) , at 4,656,590 to 4,657,090
_RS20310
HSERO_RS20315: HSERO_RS20315 - imidazole glycerol phosphate synthase, at 4,657,087 to 4,657,851
_RS20315
HSERO_RS20320: HSERO_RS20320 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 4,657,853 to 4,658,650
_RS20320
HSERO_RS20325: HSERO_RS20325 - IGP synthase, amidotransferase subunit (EC 4.3.2.10) (from data) , at 4,658,727 to 4,659,365
_RS20325
HSERO_RS20330: HSERO_RS20330 - imidazoleglycerol-phosphate dehydratase, at 4,659,427 to 4,660,023
_RS20330
Position (kb)
4657
4658
4659 Strain fitness (log2 ratio)
-1
0
1
2 at 4656.873 kb on + strand, within HSERO_RS20310 at 4657.126 kb on - strand at 4657.167 kb on - strand, within HSERO_RS20315 at 4657.331 kb on - strand, within HSERO_RS20315 at 4657.341 kb on - strand at 4657.565 kb on + strand, within HSERO_RS20315 at 4657.573 kb on - strand, within HSERO_RS20315 at 4657.721 kb on - strand, within HSERO_RS20315 at 4657.860 kb on + strand at 4657.913 kb on - strand at 4657.935 kb on - strand, within HSERO_RS20320 at 4657.961 kb on + strand, within HSERO_RS20320 at 4657.989 kb on + strand, within HSERO_RS20320 at 4658.144 kb on + strand, within HSERO_RS20320 at 4658.194 kb on + strand, within HSERO_RS20320 at 4658.330 kb on - strand, within HSERO_RS20320 at 4658.527 kb on + strand, within HSERO_RS20320 at 4658.665 kb on - strand at 4658.848 kb on + strand, within HSERO_RS20325 at 4659.000 kb on - strand, within HSERO_RS20325 at 4659.000 kb on - strand, within HSERO_RS20325 at 4659.015 kb on - strand, within HSERO_RS20325 at 4659.076 kb on + strand, within HSERO_RS20325 at 4659.127 kb on - strand, within HSERO_RS20325 at 4659.127 kb on - strand, within HSERO_RS20325 at 4659.154 kb on - strand, within HSERO_RS20325 at 4659.510 kb on - strand, within HSERO_RS20330 at 4659.529 kb on - strand, within HSERO_RS20330 at 4659.564 kb on + strand, within HSERO_RS20330 at 4659.613 kb on + strand, within HSERO_RS20330 at 4659.626 kb on - strand, within HSERO_RS20330
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 4,656,873 + HSERO_RS20310 0.56 -0.6 4,657,126 - +0.2 4,657,167 - HSERO_RS20315 0.10 +0.3 4,657,331 - HSERO_RS20315 0.32 +0.9 4,657,341 - +2.2 4,657,565 + HSERO_RS20315 0.62 +0.5 4,657,573 - HSERO_RS20315 0.64 +0.5 4,657,721 - HSERO_RS20315 0.83 +0.1 4,657,860 + -0.5 4,657,913 - +0.6 4,657,935 - HSERO_RS20320 0.10 -0.2 4,657,961 + HSERO_RS20320 0.14 +0.2 4,657,989 + HSERO_RS20320 0.17 +1.1 4,658,144 + HSERO_RS20320 0.36 +1.1 4,658,194 + HSERO_RS20320 0.43 +1.2 4,658,330 - HSERO_RS20320 0.60 +0.5 4,658,527 + HSERO_RS20320 0.84 -0.5 4,658,665 - +1.4 4,658,848 + HSERO_RS20325 0.19 +1.3 4,659,000 - HSERO_RS20325 0.43 +0.3 4,659,000 - HSERO_RS20325 0.43 -0.2 4,659,015 - HSERO_RS20325 0.45 +0.0 4,659,076 + HSERO_RS20325 0.55 +1.6 4,659,127 - HSERO_RS20325 0.63 +0.3 4,659,127 - HSERO_RS20325 0.63 +0.7 4,659,154 - HSERO_RS20325 0.67 +0.4 4,659,510 - HSERO_RS20330 0.14 -0.1 4,659,529 - HSERO_RS20330 0.17 +0.4 4,659,564 + HSERO_RS20330 0.23 +1.1 4,659,613 + HSERO_RS20330 0.31 +0.6 4,659,626 - HSERO_RS20330 0.33 -0.3
Or see this region's nucleotide sequence