Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS20300 and HSERO_RS20305 are separated by 49 nucleotides HSERO_RS20305 and HSERO_RS20310 overlap by 4 nucleotides HSERO_RS20310 and HSERO_RS20315 overlap by 4 nucleotides HSERO_RS20315 and HSERO_RS20320 are separated by 1 nucleotides HSERO_RS20320 and HSERO_RS20325 are separated by 76 nucleotides
HSERO_RS20300: HSERO_RS20300 - HIT family hydrolase, at 4,655,825 to 4,656,205
_RS20300
HSERO_RS20305: HSERO_RS20305 - phosphoribosyl-ATP pyrophosphatase, at 4,656,255 to 4,656,593
_RS20305
HSERO_RS20310: HSERO_RS20310 - phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) <small>(from data)</small>, at 4,656,590 to 4,657,090
_RS20310
HSERO_RS20315: HSERO_RS20315 - imidazole glycerol phosphate synthase, at 4,657,087 to 4,657,851
_RS20315
HSERO_RS20320: HSERO_RS20320 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) <small>(from data)</small>, at 4,657,853 to 4,658,650
_RS20320
HSERO_RS20325: HSERO_RS20325 - IGP synthase, amidotransferase subunit (EC 4.3.2.10) <small>(from data)</small>, at 4,658,727 to 4,659,365
_RS20325
Position (kb) 4657
4658 Strain fitness (log2 ratio) -1
0
1
2 at 4656.618 kb on + strand at 4656.626 kb on - strand at 4656.830 kb on - strand, within HSERO_RS20310 at 4656.835 kb on - strand, within HSERO_RS20310 at 4656.873 kb on + strand, within HSERO_RS20310 at 4657.126 kb on - strand at 4657.167 kb on - strand, within HSERO_RS20315 at 4657.331 kb on - strand, within HSERO_RS20315 at 4657.341 kb on - strand at 4657.565 kb on + strand, within HSERO_RS20315 at 4657.573 kb on - strand, within HSERO_RS20315 at 4657.721 kb on - strand, within HSERO_RS20315 at 4657.860 kb on + strand at 4657.913 kb on - strand at 4657.935 kb on - strand, within HSERO_RS20320 at 4657.961 kb on + strand, within HSERO_RS20320 at 4657.989 kb on + strand, within HSERO_RS20320 at 4658.144 kb on + strand, within HSERO_RS20320 at 4658.194 kb on + strand, within HSERO_RS20320 at 4658.330 kb on - strand, within HSERO_RS20320 at 4658.527 kb on + strand, within HSERO_RS20320 at 4658.665 kb on - strand at 4658.848 kb on + strand, within HSERO_RS20325
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 4,656,618 + -0.4 4,656,626 - -0.3 4,656,830 - HSERO_RS20310 0.48 -0.1 4,656,835 - HSERO_RS20310 0.49 +0.5 4,656,873 + HSERO_RS20310 0.56 -0.6 4,657,126 - +0.2 4,657,167 - HSERO_RS20315 0.10 +0.3 4,657,331 - HSERO_RS20315 0.32 +0.9 4,657,341 - +2.2 4,657,565 + HSERO_RS20315 0.62 +0.5 4,657,573 - HSERO_RS20315 0.64 +0.5 4,657,721 - HSERO_RS20315 0.83 +0.1 4,657,860 + -0.5 4,657,913 - +0.6 4,657,935 - HSERO_RS20320 0.10 -0.2 4,657,961 + HSERO_RS20320 0.14 +0.2 4,657,989 + HSERO_RS20320 0.17 +1.1 4,658,144 + HSERO_RS20320 0.36 +1.1 4,658,194 + HSERO_RS20320 0.43 +1.2 4,658,330 - HSERO_RS20320 0.60 +0.5 4,658,527 + HSERO_RS20320 0.84 -0.5 4,658,665 - +1.4 4,658,848 + HSERO_RS20325 0.19 +1.3
Or see this region's nucleotide sequence