Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS18215 and HSERO_RS18220 overlap by 4 nucleotides HSERO_RS18220 and HSERO_RS18225 are separated by 29 nucleotides HSERO_RS18225 and HSERO_RS18230 are separated by 4 nucleotides HSERO_RS18230 and HSERO_RS18235 are separated by 80 nucleotides
HSERO_RS18215: HSERO_RS18215 - Tfp pilus assembly transmembrane PilW protein, at 4,184,001 to 4,184,885
_RS18215
HSERO_RS18220: HSERO_RS18220 - pilus assembly protein, at 4,184,882 to 4,185,487
_RS18220
HSERO_RS18225: HSERO_RS18225 - pilus assembly protein, at 4,185,517 to 4,185,972
_RS18225
HSERO_RS18230: HSERO_RS18230 - pilus assembly protein, at 4,185,977 to 4,186,450
_RS18230
HSERO_RS18235: HSERO_RS18235 - diaminohydroxyphosphoribosylaminopyrimidine deaminase, at 4,186,531 to 4,187,652
_RS18235
Position (kb) 4185
4186 Strain fitness (log2 ratio) -1
0
1 at 4184.665 kb on + strand, within HSERO_RS18215 at 4184.754 kb on - strand, within HSERO_RS18215 at 4184.866 kb on + strand at 4184.945 kb on - strand, within HSERO_RS18220 at 4185.060 kb on + strand, within HSERO_RS18220 at 4185.127 kb on + strand, within HSERO_RS18220 at 4185.370 kb on - strand, within HSERO_RS18220 at 4185.545 kb on + strand at 4185.553 kb on - strand at 4185.646 kb on + strand, within HSERO_RS18225 at 4185.666 kb on + strand, within HSERO_RS18225 at 4185.669 kb on - strand, within HSERO_RS18225 at 4185.707 kb on - strand, within HSERO_RS18225 at 4185.787 kb on + strand, within HSERO_RS18225 at 4185.795 kb on - strand, within HSERO_RS18225 at 4185.795 kb on - strand, within HSERO_RS18225 at 4186.077 kb on - strand, within HSERO_RS18230 at 4186.084 kb on - strand, within HSERO_RS18230 at 4186.292 kb on + strand, within HSERO_RS18230 at 4186.399 kb on + strand, within HSERO_RS18230 at 4186.429 kb on - strand at 4186.485 kb on - strand at 4186.496 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 4,184,665 + HSERO_RS18215 0.75 -0.3 4,184,754 - HSERO_RS18215 0.85 -0.2 4,184,866 + +1.4 4,184,945 - HSERO_RS18220 0.10 +0.1 4,185,060 + HSERO_RS18220 0.29 +0.5 4,185,127 + HSERO_RS18220 0.40 -0.3 4,185,370 - HSERO_RS18220 0.81 +0.1 4,185,545 + -0.7 4,185,553 - -0.3 4,185,646 + HSERO_RS18225 0.28 +0.4 4,185,666 + HSERO_RS18225 0.33 -0.1 4,185,669 - HSERO_RS18225 0.33 +1.1 4,185,707 - HSERO_RS18225 0.42 +0.1 4,185,787 + HSERO_RS18225 0.59 +1.0 4,185,795 - HSERO_RS18225 0.61 +0.5 4,185,795 - HSERO_RS18225 0.61 +0.1 4,186,077 - HSERO_RS18230 0.21 -0.3 4,186,084 - HSERO_RS18230 0.23 -0.2 4,186,292 + HSERO_RS18230 0.66 +0.5 4,186,399 + HSERO_RS18230 0.89 +0.3 4,186,429 - +0.3 4,186,485 - +0.1 4,186,496 + -0.9
Or see this region's nucleotide sequence