Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS12985 and HSERO_RS12990 are separated by 132 nucleotides HSERO_RS12990 and HSERO_RS12995 overlap by 4 nucleotides HSERO_RS12995 and HSERO_RS13000 are separated by 59 nucleotides HSERO_RS13000 and HSERO_RS13005 are separated by 35 nucleotides
HSERO_RS12985: HSERO_RS12985 - L-lactate dehydrogenase, at 2,960,702 to 2,961,844
_RS12985
HSERO_RS12990: HSERO_RS12990 - aankyrin, at 2,961,977 to 2,962,648
_RS12990
HSERO_RS12995: HSERO_RS12995 - DNAase, at 2,962,645 to 2,963,424
_RS12995
HSERO_RS13000: HSERO_RS13000 - chemotaxis protein CheY, at 2,963,484 to 2,964,032
_RS13000
HSERO_RS13005: HSERO_RS13005 - histidine kinase, at 2,964,068 to 2,965,399
_RS13005
Position (kb)
2962
2963
2964 Strain fitness (log2 ratio)
-1
0
1 at 2961.663 kb on - strand, within HSERO_RS12985 at 2961.783 kb on + strand at 2961.801 kb on - strand at 2961.983 kb on - strand at 2962.147 kb on + strand, within HSERO_RS12990 at 2962.340 kb on + strand, within HSERO_RS12990 at 2962.538 kb on - strand, within HSERO_RS12990 at 2962.569 kb on - strand, within HSERO_RS12990 at 2962.611 kb on - strand at 2963.119 kb on - strand, within HSERO_RS12995 at 2963.259 kb on - strand, within HSERO_RS12995 at 2963.325 kb on + strand, within HSERO_RS12995 at 2963.480 kb on - strand at 2963.795 kb on + strand, within HSERO_RS13000 at 2963.799 kb on - strand, within HSERO_RS13000 at 2963.814 kb on + strand, within HSERO_RS13000 at 2963.847 kb on - strand, within HSERO_RS13000 at 2963.851 kb on - strand, within HSERO_RS13000
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 2,961,663 - HSERO_RS12985 0.84 -0.2 2,961,783 + -1.0 2,961,801 - -0.6 2,961,983 - -0.6 2,962,147 + HSERO_RS12990 0.25 -1.0 2,962,340 + HSERO_RS12990 0.54 -0.5 2,962,538 - HSERO_RS12990 0.83 -0.2 2,962,569 - HSERO_RS12990 0.88 -0.8 2,962,611 - +0.0 2,963,119 - HSERO_RS12995 0.61 +0.5 2,963,259 - HSERO_RS12995 0.79 -1.1 2,963,325 + HSERO_RS12995 0.87 +0.7 2,963,480 - +0.3 2,963,795 + HSERO_RS13000 0.57 +0.0 2,963,799 - HSERO_RS13000 0.57 -0.4 2,963,814 + HSERO_RS13000 0.60 +0.2 2,963,847 - HSERO_RS13000 0.66 +0.4 2,963,851 - HSERO_RS13000 0.67 +0.0
Or see this region's nucleotide sequence