Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS12635 and HSERO_RS12640 are separated by 7 nucleotides HSERO_RS12640 and HSERO_RS12645 are separated by 31 nucleotides HSERO_RS12645 and HSERO_RS12650 are separated by 214 nucleotides HSERO_RS12650 and HSERO_RS12655 are separated by 91 nucleotides
HSERO_RS12635: HSERO_RS12635 - histidine kinase, at 2,881,833 to 2,883,239
_RS12635
HSERO_RS12640: HSERO_RS12640 - hypothetical protein, at 2,883,247 to 2,883,684
_RS12640
HSERO_RS12645: HSERO_RS12645 - methyltransferase, at 2,883,716 to 2,884,903
_RS12645
HSERO_RS12650: HSERO_RS12650 - hypothetical protein, at 2,885,118 to 2,885,405
_RS12650
HSERO_RS12655: HSERO_RS12655 - pirin, at 2,885,497 to 2,886,375
_RS12655
Position (kb)
2883
2884
2885 Strain fitness (log2 ratio)
-1
0
1
2 at 2883.100 kb on - strand at 2883.106 kb on + strand at 2883.106 kb on + strand at 2883.185 kb on + strand at 2883.233 kb on + strand at 2883.524 kb on + strand, within HSERO_RS12640 at 2883.722 kb on - strand at 2883.795 kb on + strand at 2883.886 kb on + strand, within HSERO_RS12645 at 2883.912 kb on - strand, within HSERO_RS12645 at 2884.151 kb on - strand, within HSERO_RS12645 at 2884.185 kb on - strand, within HSERO_RS12645 at 2884.232 kb on - strand, within HSERO_RS12645 at 2884.318 kb on + strand, within HSERO_RS12645 at 2884.536 kb on + strand, within HSERO_RS12645 at 2884.638 kb on + strand, within HSERO_RS12645 at 2884.993 kb on - strand at 2885.180 kb on + strand, within HSERO_RS12650 at 2885.394 kb on + strand at 2885.399 kb on + strand at 2885.399 kb on + strand at 2885.407 kb on - strand at 2885.407 kb on - strand at 2885.485 kb on + strand at 2885.567 kb on + strand at 2885.599 kb on - strand, within HSERO_RS12655 at 2885.804 kb on + strand, within HSERO_RS12655 at 2885.855 kb on - strand, within HSERO_RS12655
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 2,883,100 - -0.1 2,883,106 + +0.3 2,883,106 + -0.4 2,883,185 + +0.3 2,883,233 + -0.4 2,883,524 + HSERO_RS12640 0.63 +0.3 2,883,722 - -0.1 2,883,795 + -0.1 2,883,886 + HSERO_RS12645 0.14 -0.1 2,883,912 - HSERO_RS12645 0.16 +2.0 2,884,151 - HSERO_RS12645 0.37 +0.3 2,884,185 - HSERO_RS12645 0.39 -0.1 2,884,232 - HSERO_RS12645 0.43 +1.0 2,884,318 + HSERO_RS12645 0.51 -0.0 2,884,536 + HSERO_RS12645 0.69 +0.9 2,884,638 + HSERO_RS12645 0.78 +0.9 2,884,993 - +0.7 2,885,180 + HSERO_RS12650 0.22 -0.9 2,885,394 + -0.2 2,885,399 + -0.0 2,885,399 + -0.1 2,885,407 - -0.6 2,885,407 - +0.3 2,885,485 + +0.1 2,885,567 + -0.4 2,885,599 - HSERO_RS12655 0.12 +0.3 2,885,804 + HSERO_RS12655 0.35 -0.2 2,885,855 - HSERO_RS12655 0.41 -1.5
Or see this region's nucleotide sequence