Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS12285 and HSERO_RS12290 are separated by 107 nucleotides HSERO_RS12290 and HSERO_RS12295 are separated by 130 nucleotides HSERO_RS12295 and HSERO_RS12300 are separated by 5 nucleotides HSERO_RS12300 and HSERO_RS12305 overlap by 4 nucleotides
HSERO_RS12285: HSERO_RS12285 - ABC transporter substrate-binding protein, at 2,789,404 to 2,790,618
_RS12285
HSERO_RS12290: HSERO_RS12290 - chemotaxis protein CheY, at 2,790,726 to 2,791,262
_RS12290
HSERO_RS12295: HSERO_RS12295 - histidine kinase, at 2,791,393 to 2,792,775
_RS12295
HSERO_RS12300: HSERO_RS12300 - hypothetical protein, at 2,792,781 to 2,793,638
_RS12300
HSERO_RS12305: HSERO_RS12305 - cytochrome O ubiquinol oxidase, at 2,793,635 to 2,794,111
_RS12305
Position (kb)
2791
2792
2793 Strain fitness (log2 ratio)
-1
0
1 at 2790.519 kb on + strand at 2790.640 kb on + strand at 2791.602 kb on + strand, within HSERO_RS12295 at 2792.205 kb on - strand, within HSERO_RS12295 at 2792.208 kb on + strand, within HSERO_RS12295 at 2792.221 kb on - strand, within HSERO_RS12295 at 2792.550 kb on + strand, within HSERO_RS12295 at 2792.893 kb on - strand, within HSERO_RS12300 at 2793.281 kb on - strand, within HSERO_RS12300 at 2793.296 kb on + strand, within HSERO_RS12300 at 2793.366 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 2,790,519 + -0.2 2,790,640 + +0.6 2,791,602 + HSERO_RS12295 0.15 +1.4 2,792,205 - HSERO_RS12295 0.59 +0.3 2,792,208 + HSERO_RS12295 0.59 +0.9 2,792,221 - HSERO_RS12295 0.60 -1.4 2,792,550 + HSERO_RS12295 0.84 -0.2 2,792,893 - HSERO_RS12300 0.13 -0.6 2,793,281 - HSERO_RS12300 0.58 -0.8 2,793,296 + HSERO_RS12300 0.60 +0.3 2,793,366 - -1.0
Or see this region's nucleotide sequence