Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS12130 and HSERO_RS12135 are separated by 60 nucleotides HSERO_RS12135 and HSERO_RS12140 overlap by 4 nucleotides HSERO_RS12140 and HSERO_RS12145 overlap by 4 nucleotides HSERO_RS12145 and HSERO_RS12150 are separated by 343 nucleotides
HSERO_RS12130: HSERO_RS12130 - 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22) (from data) , at 2,755,654 to 2,757,531
_RS12130
HSERO_RS12135: HSERO_RS12135 - Myo-inosose-2 dehydratase (EC 4.2.1.44) (from data) , at 2,757,592 to 2,758,512
_RS12135
HSERO_RS12140: HSERO_RS12140 - 5-deoxyglucuronate isomerase, at 2,758,509 to 2,759,303
_RS12140
HSERO_RS12145: HSERO_RS12145 - hypothetical protein, at 2,759,300 to 2,759,617
_RS12145
HSERO_RS12150: HSERO_RS12150 - iron-regulated membrane protein, at 2,759,961 to 2,761,139
_RS12150
Position (kb)
2758
2759
2760 Strain fitness (log2 ratio)
-1
0
1 at 2757.693 kb on + strand, within HSERO_RS12135 at 2757.761 kb on - strand, within HSERO_RS12135 at 2757.929 kb on - strand, within HSERO_RS12135 at 2758.215 kb on + strand, within HSERO_RS12135 at 2758.410 kb on - strand, within HSERO_RS12135 at 2758.410 kb on - strand, within HSERO_RS12135 at 2758.745 kb on + strand, within HSERO_RS12140 at 2759.026 kb on - strand, within HSERO_RS12140 at 2759.075 kb on + strand, within HSERO_RS12140 at 2759.190 kb on - strand at 2759.322 kb on + strand at 2759.359 kb on - strand, within HSERO_RS12145 at 2759.403 kb on - strand, within HSERO_RS12145 at 2759.430 kb on - strand, within HSERO_RS12145 at 2759.581 kb on + strand, within HSERO_RS12145 at 2759.814 kb on - strand at 2760.116 kb on + strand, within HSERO_RS12150 at 2760.173 kb on + strand, within HSERO_RS12150
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 2,757,693 + HSERO_RS12135 0.11 -0.2 2,757,761 - HSERO_RS12135 0.18 -0.1 2,757,929 - HSERO_RS12135 0.37 -0.4 2,758,215 + HSERO_RS12135 0.68 -0.6 2,758,410 - HSERO_RS12135 0.89 +0.0 2,758,410 - HSERO_RS12135 0.89 +0.1 2,758,745 + HSERO_RS12140 0.30 +1.4 2,759,026 - HSERO_RS12140 0.65 -0.1 2,759,075 + HSERO_RS12140 0.71 +1.0 2,759,190 - +0.8 2,759,322 + -0.3 2,759,359 - HSERO_RS12145 0.19 +0.4 2,759,403 - HSERO_RS12145 0.32 -0.1 2,759,430 - HSERO_RS12145 0.41 -1.1 2,759,581 + HSERO_RS12145 0.88 +0.6 2,759,814 - +0.3 2,760,116 + HSERO_RS12150 0.13 +0.3 2,760,173 + HSERO_RS12150 0.18 -0.1
Or see this region's nucleotide sequence