Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS10830 and HSERO_RS10835 are separated by 82 nucleotides HSERO_RS10835 and HSERO_RS10840 are separated by 76 nucleotides HSERO_RS10840 and HSERO_RS10845 are separated by 69 nucleotides
HSERO_RS10830: HSERO_RS10830 - UTP--glucose-1-phosphate uridylyltransferase, at 2,438,952 to 2,439,842
_RS10830
HSERO_RS10835: HSERO_RS10835 - peptide deformylase, at 2,439,925 to 2,440,461
_RS10835
HSERO_RS10840: HSERO_RS10840 - cyanophycin synthetase, at 2,440,538 to 2,443,111
_RS10840
HSERO_RS10845: HSERO_RS10845 - cyanophycin synthetase, at 2,443,181 to 2,445,379
_RS10845
Position (kb)
2440
2441
2442
2443
2444 Strain fitness (log2 ratio)
-1
0
1
2 at 2439.872 kb on + strand at 2440.009 kb on - strand, within HSERO_RS10835 at 2440.058 kb on + strand, within HSERO_RS10835 at 2440.071 kb on - strand, within HSERO_RS10835 at 2440.187 kb on - strand at 2440.190 kb on + strand, within HSERO_RS10835 at 2440.749 kb on + strand at 2440.759 kb on + strand at 2440.974 kb on - strand, within HSERO_RS10840 at 2441.490 kb on + strand, within HSERO_RS10840 at 2441.786 kb on - strand, within HSERO_RS10840 at 2441.996 kb on + strand, within HSERO_RS10840 at 2442.001 kb on + strand, within HSERO_RS10840 at 2442.330 kb on - strand, within HSERO_RS10840 at 2442.330 kb on - strand, within HSERO_RS10840 at 2442.359 kb on - strand, within HSERO_RS10840 at 2442.359 kb on - strand, within HSERO_RS10840 at 2442.374 kb on - strand, within HSERO_RS10840 at 2442.412 kb on + strand at 2442.488 kb on + strand, within HSERO_RS10840 at 2443.405 kb on + strand, within HSERO_RS10845 at 2443.834 kb on - strand, within HSERO_RS10845 at 2444.024 kb on - strand, within HSERO_RS10845
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 2,439,872 + -0.3 2,440,009 - HSERO_RS10835 0.16 -0.5 2,440,058 + HSERO_RS10835 0.25 -0.2 2,440,071 - HSERO_RS10835 0.27 -0.4 2,440,187 - +1.0 2,440,190 + HSERO_RS10835 0.49 -0.0 2,440,749 + -0.2 2,440,759 + +0.4 2,440,974 - HSERO_RS10840 0.17 -0.6 2,441,490 + HSERO_RS10840 0.37 +0.4 2,441,786 - HSERO_RS10840 0.48 +0.6 2,441,996 + HSERO_RS10840 0.57 +0.7 2,442,001 + HSERO_RS10840 0.57 +0.3 2,442,330 - HSERO_RS10840 0.70 +0.9 2,442,330 - HSERO_RS10840 0.70 +0.2 2,442,359 - HSERO_RS10840 0.71 -0.6 2,442,359 - HSERO_RS10840 0.71 +1.6 2,442,374 - HSERO_RS10840 0.71 +0.2 2,442,412 + +1.4 2,442,488 + HSERO_RS10840 0.76 +1.4 2,443,405 + HSERO_RS10845 0.10 +1.8 2,443,834 - HSERO_RS10845 0.30 -0.1 2,444,024 - HSERO_RS10845 0.38 -0.7
Or see this region's nucleotide sequence