Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS10775 and HSERO_RS10780 are separated by 137 nucleotides HSERO_RS10780 and HSERO_RS10785 are separated by 48 nucleotides HSERO_RS10785 and HSERO_RS10790 are separated by 29 nucleotides
HSERO_RS10775: HSERO_RS10775 - cysteine ABC transporter substrate-binding protein, at 2,421,553 to 2,422,356
_RS10775
HSERO_RS10780: HSERO_RS10780 - glutathione ABC transporter ATP-binding protein, at 2,422,494 to 2,424,476
_RS10780
HSERO_RS10785: HSERO_RS10785 - N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase, at 2,424,525 to 2,425,415
_RS10785
HSERO_RS10790: HSERO_RS10790 - succinyl-diaminopimelate desuccinylase, at 2,425,445 to 2,426,572
_RS10790
Position (kb)
2422
2423
2424
2425 Strain fitness (log2 ratio)
-1
0
1 at 2421.579 kb on + strand at 2421.644 kb on + strand, within HSERO_RS10775 at 2421.652 kb on - strand, within HSERO_RS10775 at 2422.000 kb on - strand, within HSERO_RS10775 at 2422.099 kb on + strand, within HSERO_RS10775 at 2422.099 kb on + strand, within HSERO_RS10775 at 2422.142 kb on + strand, within HSERO_RS10775 at 2422.252 kb on + strand, within HSERO_RS10775 at 2422.473 kb on - strand at 2422.500 kb on - strand at 2422.500 kb on - strand at 2422.625 kb on + strand at 2423.207 kb on + strand, within HSERO_RS10780 at 2423.365 kb on + strand, within HSERO_RS10780 at 2423.400 kb on - strand, within HSERO_RS10780 at 2424.378 kb on + strand at 2424.383 kb on + strand at 2424.511 kb on - strand at 2424.511 kb on - strand at 2424.656 kb on + strand, within HSERO_RS10785 at 2424.973 kb on + strand, within HSERO_RS10785 at 2425.236 kb on - strand, within HSERO_RS10785 at 2425.382 kb on - strand at 2425.419 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 2,421,579 + +0.0 2,421,644 + HSERO_RS10775 0.11 -0.3 2,421,652 - HSERO_RS10775 0.12 -0.2 2,422,000 - HSERO_RS10775 0.56 -0.3 2,422,099 + HSERO_RS10775 0.68 -0.5 2,422,099 + HSERO_RS10775 0.68 -0.3 2,422,142 + HSERO_RS10775 0.73 -0.6 2,422,252 + HSERO_RS10775 0.87 -0.6 2,422,473 - -0.1 2,422,500 - -0.3 2,422,500 - -0.2 2,422,625 + +0.3 2,423,207 + HSERO_RS10780 0.36 +0.2 2,423,365 + HSERO_RS10780 0.44 +1.6 2,423,400 - HSERO_RS10780 0.46 -0.1 2,424,378 + -0.9 2,424,383 + +0.0 2,424,511 - -0.0 2,424,511 - +1.3 2,424,656 + HSERO_RS10785 0.15 -1.0 2,424,973 + HSERO_RS10785 0.50 -0.7 2,425,236 - HSERO_RS10785 0.80 +0.5 2,425,382 - -0.2 2,425,419 - -0.5
Or see this region's nucleotide sequence