Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS10305 and HSERO_RS10310 overlap by 8 nucleotides HSERO_RS10310 and HSERO_RS10315 are separated by 250 nucleotides HSERO_RS10315 and HSERO_RS10320 are separated by 120 nucleotides
HSERO_RS10305: HSERO_RS10305 - flagellar motor switch protein FliG, at 2,321,913 to 2,322,908
_RS10305
HSERO_RS10310: HSERO_RS10310 - flagellar M-ring protein FliF, at 2,322,901 to 2,324,682
_RS10310
HSERO_RS10315: HSERO_RS10315 - flagellar hook-basal body protein FliE, at 2,324,933 to 2,325,289
_RS10315
HSERO_RS10320: HSERO_RS10320 - flagellar brake protein YcgR, at 2,325,410 to 2,326,165
_RS10320
Position (kb)
2322
2323
2324
2325 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2321.901 kb on + strand at 2322.144 kb on + strand, within HSERO_RS10305 at 2322.264 kb on + strand, within HSERO_RS10305 at 2322.418 kb on - strand, within HSERO_RS10305 at 2322.418 kb on - strand, within HSERO_RS10305 at 2322.666 kb on + strand, within HSERO_RS10305 at 2322.953 kb on + strand at 2322.986 kb on + strand at 2322.986 kb on + strand at 2322.994 kb on - strand at 2322.994 kb on - strand at 2323.038 kb on - strand at 2323.208 kb on + strand, within HSERO_RS10310 at 2323.286 kb on + strand, within HSERO_RS10310 at 2323.502 kb on + strand, within HSERO_RS10310 at 2323.525 kb on + strand, within HSERO_RS10310 at 2323.546 kb on + strand, within HSERO_RS10310 at 2324.305 kb on - strand, within HSERO_RS10310 at 2324.347 kb on + strand, within HSERO_RS10310 at 2324.554 kb on - strand at 2324.602 kb on - strand at 2324.616 kb on + strand at 2324.735 kb on + strand at 2324.735 kb on + strand at 2324.764 kb on + strand at 2324.805 kb on - strand at 2324.994 kb on - strand at 2325.096 kb on + strand, within HSERO_RS10315 at 2325.106 kb on + strand, within HSERO_RS10315 at 2325.106 kb on + strand, within HSERO_RS10315 at 2325.249 kb on - strand, within HSERO_RS10315 at 2325.258 kb on - strand at 2325.258 kb on - strand at 2325.528 kb on + strand, within HSERO_RS10320
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 2,321,901 + +0.3 2,322,144 + HSERO_RS10305 0.23 +1.0 2,322,264 + HSERO_RS10305 0.35 +0.6 2,322,418 - HSERO_RS10305 0.51 +1.1 2,322,418 - HSERO_RS10305 0.51 +0.1 2,322,666 + HSERO_RS10305 0.76 +0.8 2,322,953 + +1.4 2,322,986 + +1.2 2,322,986 + +1.0 2,322,994 - +1.5 2,322,994 - +0.7 2,323,038 - +1.2 2,323,208 + HSERO_RS10310 0.17 +2.2 2,323,286 + HSERO_RS10310 0.22 +1.0 2,323,502 + HSERO_RS10310 0.34 +1.5 2,323,525 + HSERO_RS10310 0.35 +0.9 2,323,546 + HSERO_RS10310 0.36 +0.9 2,324,305 - HSERO_RS10310 0.79 -0.6 2,324,347 + HSERO_RS10310 0.81 +1.0 2,324,554 - +0.8 2,324,602 - +0.4 2,324,616 + +0.9 2,324,735 + +0.7 2,324,735 + +0.9 2,324,764 + +0.9 2,324,805 - +0.4 2,324,994 - +3.8 2,325,096 + HSERO_RS10315 0.46 -0.5 2,325,106 + HSERO_RS10315 0.48 +0.1 2,325,106 + HSERO_RS10315 0.48 +0.4 2,325,249 - HSERO_RS10315 0.89 +0.8 2,325,258 - +1.6 2,325,258 - +0.1 2,325,528 + HSERO_RS10320 0.16 +0.1
Or see this region's nucleotide sequence