Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS10175 and HSERO_RS10180 are separated by 183 nucleotides HSERO_RS10180 and HSERO_RS10185 are separated by 6 nucleotides HSERO_RS10185 and HSERO_RS10190 are separated by 10 nucleotides HSERO_RS10190 and HSERO_RS10195 are separated by 39 nucleotides
HSERO_RS10175: HSERO_RS10175 - flagellar basal body P-ring biosynthesis protein FlgA, at 2,296,248 to 2,297,012
_RS10175
HSERO_RS10180: HSERO_RS10180 - flagellar biosynthesis protein FlgB, at 2,297,196 to 2,297,624
_RS10180
HSERO_RS10185: HSERO_RS10185 - flagellar basal body rod protein FlgC, at 2,297,631 to 2,298,035
_RS10185
HSERO_RS10190: HSERO_RS10190 - flagellar basal body rod modification protein FlgD, at 2,298,046 to 2,298,768
_RS10190
HSERO_RS10195: HSERO_RS10195 - flagellar hook protein FlgE, at 2,298,808 to 2,300,106
_RS10195
Position (kb)
2297
2298
2299 Strain fitness (log2 ratio)
-1
0
1
2 at 2296.688 kb on - strand, within HSERO_RS10175 at 2296.739 kb on + strand, within HSERO_RS10175 at 2296.759 kb on + strand, within HSERO_RS10175 at 2296.822 kb on + strand, within HSERO_RS10175 at 2296.885 kb on - strand, within HSERO_RS10175 at 2296.914 kb on + strand, within HSERO_RS10175 at 2296.938 kb on + strand at 2297.400 kb on + strand at 2297.481 kb on + strand, within HSERO_RS10180 at 2297.582 kb on - strand at 2297.596 kb on - strand at 2297.600 kb on + strand at 2297.752 kb on - strand, within HSERO_RS10185 at 2297.782 kb on - strand, within HSERO_RS10185 at 2297.855 kb on - strand, within HSERO_RS10185 at 2297.972 kb on - strand, within HSERO_RS10185 at 2298.044 kb on - strand at 2298.064 kb on + strand at 2298.064 kb on + strand at 2298.145 kb on - strand, within HSERO_RS10190 at 2298.273 kb on + strand, within HSERO_RS10190 at 2298.454 kb on - strand, within HSERO_RS10190 at 2298.593 kb on - strand, within HSERO_RS10190 at 2298.798 kb on + strand at 2298.805 kb on - strand at 2298.980 kb on + strand, within HSERO_RS10195 at 2298.982 kb on - strand, within HSERO_RS10195
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 2,296,688 - HSERO_RS10175 0.58 +0.6 2,296,739 + HSERO_RS10175 0.64 +0.7 2,296,759 + HSERO_RS10175 0.67 +0.8 2,296,822 + HSERO_RS10175 0.75 +0.9 2,296,885 - HSERO_RS10175 0.83 +0.9 2,296,914 + HSERO_RS10175 0.87 +0.8 2,296,938 + +0.5 2,297,400 + +2.1 2,297,481 + HSERO_RS10180 0.66 +0.8 2,297,582 - +0.2 2,297,596 - +0.3 2,297,600 + +0.6 2,297,752 - HSERO_RS10185 0.30 +0.4 2,297,782 - HSERO_RS10185 0.37 +0.4 2,297,855 - HSERO_RS10185 0.55 +0.3 2,297,972 - HSERO_RS10185 0.84 +0.1 2,298,044 - +0.5 2,298,064 + +0.8 2,298,064 + +0.7 2,298,145 - HSERO_RS10190 0.14 +0.3 2,298,273 + HSERO_RS10190 0.31 +0.6 2,298,454 - HSERO_RS10190 0.56 -0.2 2,298,593 - HSERO_RS10190 0.76 -0.3 2,298,798 + +0.4 2,298,805 - +0.9 2,298,980 + HSERO_RS10195 0.13 +0.5 2,298,982 - HSERO_RS10195 0.13 -0.4
Or see this region's nucleotide sequence