Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS10155 and HSERO_RS10160 are separated by 5 nucleotides HSERO_RS10160 and HSERO_RS10165 are separated by 89 nucleotides HSERO_RS10165 and HSERO_RS10170 are separated by 24 nucleotides HSERO_RS10170 and HSERO_RS10175 are separated by 164 nucleotides
HSERO_RS10155: HSERO_RS10155 - antiactivator of flagellar biosynthesis FleN protein, at 2,293,653 to 2,294,453
_RS10155
HSERO_RS10160: HSERO_RS10160 - flagellar biosynthesis sigma factor, at 2,294,459 to 2,295,193
_RS10160
HSERO_RS10165: HSERO_RS10165 - flagellar biosynthesis protein, at 2,295,283 to 2,295,741
_RS10165
HSERO_RS10170: HSERO_RS10170 - flagellar biosynthesis anti-sigma factor FlgM, at 2,295,766 to 2,296,083
_RS10170
HSERO_RS10175: HSERO_RS10175 - flagellar basal body P-ring biosynthesis protein FlgA, at 2,296,248 to 2,297,012
_RS10175
Position (kb)
2295
2296 Strain fitness (log2 ratio)
-1
0
1
2 at 2294.310 kb on + strand, within HSERO_RS10155 at 2294.318 kb on - strand, within HSERO_RS10155 at 2294.647 kb on + strand, within HSERO_RS10160 at 2294.730 kb on + strand, within HSERO_RS10160 at 2294.805 kb on + strand, within HSERO_RS10160 at 2294.815 kb on + strand, within HSERO_RS10160 at 2295.322 kb on + strand at 2295.433 kb on + strand, within HSERO_RS10165 at 2295.433 kb on + strand, within HSERO_RS10165 at 2295.556 kb on + strand, within HSERO_RS10165 at 2295.750 kb on + strand at 2296.186 kb on - strand at 2296.381 kb on - strand, within HSERO_RS10175 at 2296.382 kb on - strand, within HSERO_RS10175 at 2296.587 kb on + strand, within HSERO_RS10175 at 2296.688 kb on - strand, within HSERO_RS10175 at 2296.739 kb on + strand, within HSERO_RS10175
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 2,294,310 + HSERO_RS10155 0.82 +0.3 2,294,318 - HSERO_RS10155 0.83 -0.1 2,294,647 + HSERO_RS10160 0.26 -0.3 2,294,730 + HSERO_RS10160 0.37 +0.6 2,294,805 + HSERO_RS10160 0.47 +0.2 2,294,815 + HSERO_RS10160 0.48 -0.1 2,295,322 + +0.4 2,295,433 + HSERO_RS10165 0.33 +1.1 2,295,433 + HSERO_RS10165 0.33 -0.2 2,295,556 + HSERO_RS10165 0.59 +2.0 2,295,750 + -0.2 2,296,186 - +0.4 2,296,381 - HSERO_RS10175 0.17 +0.3 2,296,382 - HSERO_RS10175 0.18 +1.9 2,296,587 + HSERO_RS10175 0.44 +1.0 2,296,688 - HSERO_RS10175 0.58 +0.6 2,296,739 + HSERO_RS10175 0.64 +0.7
Or see this region's nucleotide sequence