Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS09450 and HSERO_RS09455 are separated by 63 nucleotides HSERO_RS09455 and HSERO_RS09460 are separated by 176 nucleotides HSERO_RS09460 and HSERO_RS09465 are separated by 265 nucleotides
HSERO_RS09450: HSERO_RS09450 - hypothetical protein, at 2,148,638 to 2,149,837
_RS09450
HSERO_RS09455: HSERO_RS09455 - NAD synthetase, at 2,149,901 to 2,151,958
_RS09455
HSERO_RS09460: HSERO_RS09460 - nitrogen regulatory protein P-II 1, at 2,152,135 to 2,152,473
_RS09460
HSERO_RS09465: HSERO_RS09465 - aldehyde dehydrogenase, at 2,152,739 to 2,154,259
_RS09465
Position (kb)
2149
2150
2151
2152 Strain fitness (log2 ratio)
-2
-1
0
1 at 2149.076 kb on - strand, within HSERO_RS09450 at 2149.086 kb on - strand, within HSERO_RS09450 at 2149.140 kb on - strand, within HSERO_RS09450 at 2149.425 kb on + strand, within HSERO_RS09450 at 2149.425 kb on + strand, within HSERO_RS09450 at 2149.464 kb on + strand, within HSERO_RS09450 at 2149.479 kb on + strand, within HSERO_RS09450 at 2149.515 kb on + strand, within HSERO_RS09450 at 2149.611 kb on - strand, within HSERO_RS09450 at 2149.630 kb on + strand, within HSERO_RS09450 at 2149.674 kb on + strand, within HSERO_RS09450 at 2149.682 kb on - strand, within HSERO_RS09450 at 2149.719 kb on - strand at 2149.887 kb on + strand at 2149.887 kb on + strand at 2149.887 kb on + strand at 2149.895 kb on - strand at 2150.485 kb on - strand, within HSERO_RS09455 at 2150.498 kb on - strand, within HSERO_RS09455 at 2150.635 kb on + strand, within HSERO_RS09455 at 2151.079 kb on + strand, within HSERO_RS09455 at 2151.548 kb on - strand, within HSERO_RS09455 at 2151.730 kb on + strand at 2151.742 kb on + strand, within HSERO_RS09455 at 2151.822 kb on - strand at 2152.787 kb on - strand at 2152.828 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 2,149,076 - HSERO_RS09450 0.36 +0.9 2,149,086 - HSERO_RS09450 0.37 +1.7 2,149,140 - HSERO_RS09450 0.42 -0.0 2,149,425 + HSERO_RS09450 0.66 +0.4 2,149,425 + HSERO_RS09450 0.66 -0.3 2,149,464 + HSERO_RS09450 0.69 +0.6 2,149,479 + HSERO_RS09450 0.70 -0.4 2,149,515 + HSERO_RS09450 0.73 +0.4 2,149,611 - HSERO_RS09450 0.81 +0.1 2,149,630 + HSERO_RS09450 0.83 +0.4 2,149,674 + HSERO_RS09450 0.86 -1.2 2,149,682 - HSERO_RS09450 0.87 -2.0 2,149,719 - -0.2 2,149,887 + +0.0 2,149,887 + +0.3 2,149,887 + +0.1 2,149,895 - +0.1 2,150,485 - HSERO_RS09455 0.28 -0.8 2,150,498 - HSERO_RS09455 0.29 -1.3 2,150,635 + HSERO_RS09455 0.36 -0.3 2,151,079 + HSERO_RS09455 0.57 +0.3 2,151,548 - HSERO_RS09455 0.80 -1.3 2,151,730 + -0.5 2,151,742 + HSERO_RS09455 0.89 -0.4 2,151,822 - -1.7 2,152,787 - -0.7 2,152,828 + +0.0
Or see this region's nucleotide sequence