Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS09280 and HSERO_RS09285 are separated by 525 nucleotides HSERO_RS09285 and HSERO_RS09290 are separated by 237 nucleotides HSERO_RS09290 and HSERO_RS09295 are separated by 110 nucleotides HSERO_RS09295 and HSERO_RS09300 are separated by 124 nucleotides
HSERO_RS09280: HSERO_RS09280 - deoxyribodipyrimidine photo-lyase, at 2,109,364 to 2,110,848
_RS09280
HSERO_RS09285: HSERO_RS09285 - tRNA-Leu, at 2,111,374 to 2,111,458
_RS09285
HSERO_RS09290: HSERO_RS09290 - ribonuclease R, at 2,111,696 to 2,114,215
_RS09290
HSERO_RS09295: HSERO_RS09295 - 23S rRNA (guanosine-2'-O-)-methyltransferase, at 2,114,326 to 2,115,063
_RS09295
HSERO_RS09300: HSERO_RS09300 - serine O-acetyltransferase, at 2,115,188 to 2,115,940
_RS09300
Position (kb)
2111
2112
2113
2114
2115 Strain fitness (log2 ratio)
-1
0
1 at 2110.703 kb on + strand at 2110.705 kb on + strand at 2110.711 kb on - strand at 2110.812 kb on + strand at 2111.000 kb on + strand at 2111.089 kb on - strand at 2111.477 kb on - strand at 2111.562 kb on - strand at 2111.670 kb on + strand at 2111.678 kb on - strand at 2111.952 kb on + strand, within HSERO_RS09290 at 2112.213 kb on + strand, within HSERO_RS09290 at 2112.597 kb on - strand, within HSERO_RS09290 at 2112.642 kb on - strand, within HSERO_RS09290 at 2112.836 kb on - strand, within HSERO_RS09290 at 2112.906 kb on - strand, within HSERO_RS09290 at 2113.080 kb on + strand, within HSERO_RS09290 at 2113.080 kb on - strand, within HSERO_RS09290 at 2113.281 kb on - strand, within HSERO_RS09290 at 2114.127 kb on - strand at 2114.521 kb on + strand, within HSERO_RS09295 at 2114.535 kb on - strand, within HSERO_RS09295 at 2114.683 kb on + strand, within HSERO_RS09295 at 2114.730 kb on - strand, within HSERO_RS09295 at 2114.813 kb on + strand, within HSERO_RS09295 at 2114.978 kb on + strand, within HSERO_RS09295 at 2115.196 kb on - strand at 2115.207 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 2,110,703 + +0.1 2,110,705 + -0.3 2,110,711 - +1.1 2,110,812 + +0.0 2,111,000 + +0.3 2,111,089 - +0.1 2,111,477 - -0.5 2,111,562 - -0.9 2,111,670 + +0.1 2,111,678 - +0.1 2,111,952 + HSERO_RS09290 0.10 +1.1 2,112,213 + HSERO_RS09290 0.21 +1.1 2,112,597 - HSERO_RS09290 0.36 +1.0 2,112,642 - HSERO_RS09290 0.38 +0.3 2,112,836 - HSERO_RS09290 0.45 +0.9 2,112,906 - HSERO_RS09290 0.48 -0.9 2,113,080 + HSERO_RS09290 0.55 +0.9 2,113,080 - HSERO_RS09290 0.55 +0.4 2,113,281 - HSERO_RS09290 0.63 +0.1 2,114,127 - +0.2 2,114,521 + HSERO_RS09295 0.26 -0.8 2,114,535 - HSERO_RS09295 0.28 -1.1 2,114,683 + HSERO_RS09295 0.48 +0.6 2,114,730 - HSERO_RS09295 0.55 +0.2 2,114,813 + HSERO_RS09295 0.66 +0.7 2,114,978 + HSERO_RS09295 0.88 -0.3 2,115,196 - -0.3 2,115,207 - -0.3
Or see this region's nucleotide sequence