Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS08325 and HSERO_RS08330 are separated by 166 nucleotides HSERO_RS08330 and HSERO_RS08335 are separated by 148 nucleotides HSERO_RS08335 and HSERO_RS08340 are separated by 147 nucleotides HSERO_RS08340 and HSERO_RS08345 are separated by 91 nucleotides
HSERO_RS08325: HSERO_RS08325 - hypothetical protein, at 1,897,462 to 1,898,241
_RS08325
HSERO_RS08330: HSERO_RS08330 - hypothetical protein, at 1,898,408 to 1,898,752
_RS08330
HSERO_RS08335: HSERO_RS08335 - 4-hydroxyphenylpyruvate dioxygenase, at 1,898,901 to 1,899,995
_RS08335
HSERO_RS08340: HSERO_RS08340 - AsnC family transcriptional regulator, at 1,900,143 to 1,900,661
_RS08340
HSERO_RS08345: HSERO_RS08345 - AraC family transcriptional regulator, at 1,900,753 to 1,901,862
_RS08345
Position (kb)
1898
1899
1900 Strain fitness (log2 ratio)
-1
0
1 at 1897.905 kb on + strand, within HSERO_RS08325 at 1897.945 kb on + strand, within HSERO_RS08325 at 1897.984 kb on + strand, within HSERO_RS08325 at 1898.250 kb on + strand at 1898.391 kb on + strand at 1898.393 kb on - strand at 1898.393 kb on - strand at 1898.404 kb on - strand at 1898.425 kb on + strand at 1898.439 kb on - strand at 1898.818 kb on + strand at 1898.937 kb on - strand at 1899.176 kb on - strand, within HSERO_RS08335 at 1899.228 kb on + strand, within HSERO_RS08335 at 1899.507 kb on - strand, within HSERO_RS08335 at 1899.992 kb on - strand at 1900.352 kb on + strand, within HSERO_RS08340 at 1900.423 kb on + strand, within HSERO_RS08340 at 1900.473 kb on - strand, within HSERO_RS08340 at 1900.473 kb on - strand, within HSERO_RS08340 at 1900.580 kb on + strand, within HSERO_RS08340 at 1900.638 kb on - strand at 1900.651 kb on + strand at 1900.656 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 1,897,905 + HSERO_RS08325 0.57 -0.3 1,897,945 + HSERO_RS08325 0.62 -0.4 1,897,984 + HSERO_RS08325 0.67 -0.0 1,898,250 + +0.1 1,898,391 + +0.1 1,898,393 - +0.3 1,898,393 - -0.4 1,898,404 - -0.6 1,898,425 + -0.0 1,898,439 - -0.1 1,898,818 + +0.1 1,898,937 - +0.3 1,899,176 - HSERO_RS08335 0.25 +0.5 1,899,228 + HSERO_RS08335 0.30 +1.3 1,899,507 - HSERO_RS08335 0.55 -0.1 1,899,992 - -0.0 1,900,352 + HSERO_RS08340 0.40 -1.2 1,900,423 + HSERO_RS08340 0.54 -0.6 1,900,473 - HSERO_RS08340 0.64 -0.4 1,900,473 - HSERO_RS08340 0.64 -0.0 1,900,580 + HSERO_RS08340 0.84 +0.2 1,900,638 - -0.4 1,900,651 + -0.5 1,900,656 + +0.7
Or see this region's nucleotide sequence