Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS08065 and HSERO_RS08070 are separated by 9 nucleotides HSERO_RS08070 and HSERO_RS08075 overlap by 4 nucleotides HSERO_RS08075 and HSERO_RS08080 are separated by 129 nucleotides
HSERO_RS08065: HSERO_RS08065 - branched-chain amino acid ABC transporter substrate-binding protein, at 1,843,830 to 1,845,014
_RS08065
HSERO_RS08070: HSERO_RS08070 - endonuclease IV, at 1,845,024 to 1,845,674
_RS08070
HSERO_RS08075: HSERO_RS08075 - ferredoxin, at 1,845,671 to 1,846,393
_RS08075
HSERO_RS08080: HSERO_RS08080 - poly[D(-)-3-hydroxyalkanoate] depolymerase, at 1,846,523 to 1,847,758
_RS08080
Position (kb) 1845
1846
1847 Strain fitness (log2 ratio) -1
0
1 at 1845.130 kb on + strand, within HSERO_RS08070 at 1845.152 kb on - strand, within HSERO_RS08070 at 1845.454 kb on + strand, within HSERO_RS08070 at 1845.571 kb on + strand, within HSERO_RS08070 at 1845.595 kb on + strand, within HSERO_RS08070 at 1845.926 kb on - strand, within HSERO_RS08075 at 1846.628 kb on - strand at 1846.926 kb on - strand, within HSERO_RS08080 at 1847.006 kb on - strand, within HSERO_RS08080 at 1847.040 kb on + strand, within HSERO_RS08080 at 1847.048 kb on - strand, within HSERO_RS08080
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 1,845,130 + HSERO_RS08070 0.16 -0.1 1,845,152 - HSERO_RS08070 0.20 +1.3 1,845,454 + HSERO_RS08070 0.66 -0.4 1,845,571 + HSERO_RS08070 0.84 -0.1 1,845,595 + HSERO_RS08070 0.88 -0.4 1,845,926 - HSERO_RS08075 0.35 +0.4 1,846,628 - -0.4 1,846,926 - HSERO_RS08080 0.33 +0.2 1,847,006 - HSERO_RS08080 0.39 -0.8 1,847,040 + HSERO_RS08080 0.42 +0.1 1,847,048 - HSERO_RS08080 0.42 -0.6
Or see this region's nucleotide sequence