Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS05970 and HSERO_RS05975 are separated by 15 nucleotides HSERO_RS05975 and HSERO_RS05980 are separated by 132 nucleotides HSERO_RS05980 and HSERO_RS05985 are separated by 497 nucleotides
HSERO_RS05970: HSERO_RS05970 - ABC transporter, at 1,354,340 to 1,355,107
_RS05970
HSERO_RS05975: HSERO_RS05975 - amino acid ABC transporter ATPase, at 1,355,123 to 1,355,848
_RS05975
HSERO_RS05980: HSERO_RS05980 - aldehyde dehydrogenase, at 1,355,981 to 1,356,445
_RS05980
HSERO_RS05985: HSERO_RS05985 - hypothetical protein, at 1,356,943 to 1,357,140
_RS05985
Position (kb)
1355
1356
1357 Strain fitness (log2 ratio)
-1
0
1 at 1355.102 kb on + strand at 1355.114 kb on + strand at 1355.851 kb on - strand at 1355.859 kb on - strand at 1356.018 kb on + strand at 1356.025 kb on - strand at 1356.255 kb on - strand, within HSERO_RS05980 at 1356.316 kb on + strand, within HSERO_RS05980 at 1356.448 kb on - strand at 1356.470 kb on - strand at 1356.586 kb on - strand at 1356.586 kb on - strand at 1356.629 kb on + strand at 1356.637 kb on - strand at 1356.674 kb on + strand at 1356.676 kb on - strand at 1356.757 kb on - strand at 1356.845 kb on - strand at 1356.847 kb on - strand at 1356.847 kb on - strand at 1356.894 kb on - strand at 1356.965 kb on + strand, within HSERO_RS05985 at 1356.992 kb on - strand, within HSERO_RS05985 at 1357.025 kb on - strand, within HSERO_RS05985 at 1357.025 kb on - strand, within HSERO_RS05985 at 1357.042 kb on + strand, within HSERO_RS05985 at 1357.079 kb on + strand, within HSERO_RS05985 at 1357.182 kb on + strand at 1357.217 kb on - strand at 1357.232 kb on + strand at 1357.263 kb on + strand at 1357.391 kb on - strand at 1357.391 kb on - strand at 1357.436 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 1,355,102 + -0.2 1,355,114 + +0.5 1,355,851 - +0.5 1,355,859 - +0.1 1,356,018 + +0.7 1,356,025 - +0.4 1,356,255 - HSERO_RS05980 0.59 +0.7 1,356,316 + HSERO_RS05980 0.72 -0.5 1,356,448 - -0.2 1,356,470 - -0.0 1,356,586 - +0.9 1,356,586 - -0.4 1,356,629 + +0.1 1,356,637 - +0.8 1,356,674 + -0.4 1,356,676 - +0.1 1,356,757 - +0.4 1,356,845 - -0.2 1,356,847 - -0.2 1,356,847 - +0.3 1,356,894 - -0.1 1,356,965 + HSERO_RS05985 0.11 +0.3 1,356,992 - HSERO_RS05985 0.25 +0.5 1,357,025 - HSERO_RS05985 0.41 -0.7 1,357,025 - HSERO_RS05985 0.41 +0.2 1,357,042 + HSERO_RS05985 0.50 -0.3 1,357,079 + HSERO_RS05985 0.69 +0.1 1,357,182 + -0.3 1,357,217 - -0.4 1,357,232 + -1.3 1,357,263 + +0.0 1,357,391 - -0.7 1,357,391 - +0.1 1,357,436 - -0.8
Or see this region's nucleotide sequence